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National Institute of Environmental Health SciencesNational Institutes of Health
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Research Methods

Host Defense Group

Protein Discovery in Innate Immunity Signaling

  • Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
  • Affinity precipitation and immunoprecipitation
  • Immobilized metal affinity chromatography
  • Tandem mass spectrometry
  • Two-Dimensional Polyacrylamide Gel Electrophoresis

Role of Cholesterol Trafficking and Lipid Rafts in Innate Immunity

  • Raft isolation
  • Confocal microscopy
  • RNA interference
  • Cholesterol quantitation
  • Rho GTPase assays
  • Mouse models
  • Macrophage and neutrophil isolation Department of Health & Human Services National Institutes of Health
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Last Reviewed: January 02, 2007