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Selected Publications

DNA Repair & Nucleic Acid Enzymology Group

  1. Wilson, S.H. and Kuff, E.L. A novel DNA polymerase activity found in association with intracisternal A-type particles. Proc. Natl. Acad. Sci. USA, 69:1531-153 6, 1972.  [Abstract] ( Exit NIEHS
  2. Matsukage, A., Bohn, E.W., and Wilson, S.H. Multiple forms of DNA polymerase in mouse myeloma. Proc. Natl. Acad. Sci. USA, 71:578-582, 1974.  [Abstract] ( Exit NIEHS
  3. Tanabe, K., Bohn, E.W., and Wilson, S.H. Steady-state kinetics of mouse DNA polymerase β. Biochemistry, 18:3401-3407, 1979.  [Abstract] ( Exit NIEHS
  4. Zmudzka, B.Z., SenGupta, D., Matsukage, A., Cobianchi, F., Kumar, P., and Wilson, S.H. Structure of rat DNA polymerase β revealed by partial amino acid sequencing and cDNA cloning. Proc. Natl. Acad. Sci. USA, 83:5106-5110, 1986.  [Abstract] ( Exit NIEHS
  5. Widen, S., Kedar, P., and Wilson, S.H. Human β-polymerase gene: Structure of the 5' flanking region and active promoter. J. Biol. Chem., 263:16992-16998, 1988.  [Abstract] ( Exit NIEHS
  6. Beard, W.A. and Wilson, S.H. Kinetic analysis of template primer interactions with recombinant forms of HIV-1 reverse transcriptase. Biochemistry, 32:9745-9753, 1993.  [Abstract] ( Exit NIEHS
  7. Narayan, S., Widen, S.G., Beard, W.A., and Wilson, S.H. RNA polymerse II transcription: Rate of promoter clearance is enhanced by a purified activating transcription factor/cAMP response element-binding protein. J. Biol. Chem., 269, 12755-12763, 1994.  [Abstract] ( Exit NIEHS
  8. Sawaya, M.R., Pelletier, H., Kumar, A., Wilson, S.H., and Kraut, J. Crystal structure of rat DNA polymerase β: Evidence for a common polymerase mechanism. Science, 264:1930-1935, 1994.  [Abstract] ( Exit NIEHS
  9. Pelletier, H., Sawaya, M.R., Kumar, A., Wilson, S.H., and Kraut, J. Structures of ternary complexes of rat DNA polymerase β, a DNA template-primer, and ddCTP. Science, 264:1891-1903, 1994.  [Abstract] ( Exit NIEHS
  10. Singhal, R.K., Prasad, R., and Wilson, S.H. DNA polymerase β conducts the gap-filling step in uracil-initiated base excision repair in a bovine testis nuclear extract. J. Biol. Chem., 270:949-957, 1995.  [Abstract] ( Exit NIEHS
  11. Kunkel, T.A. and Wilson, S.H. DNA Repair. Push and pull of base flipping. Nature, 384:25-26, 1996.  [Abstract] ( Exit NIEHS
  12. Sobol, R.W., Horton, J.K., Kühn, R., Gu, H., Singhal, R.K., Prasad, R., Rajewsky, K., and Wilson, S.H. Requirement of mammalian DNA polymerase-β in base-excision repair. Nature, 379:183-186, 1996.  [Abstract] ( Exit NIEHS
  13. He, F., Narayan, S., and Wilson, S.H. Purification and characterization of a DNA polymerase β promoter initiator element-binding transcription factor from bovine testis. Biochemistry, 35:1775-1782, 1996.  [Abstract] ( Exit NIEHS
  14. Srivastava, D.K., Evans, R.K., Kumar, A., Beard, W.A., and Wilson, S.H. dNTP binding site in rat DNA polymerase β revealed by controlled proteolysis and azido photoprobe cross-linking. Biochemistry, 35:3728-3734, 1996.  [Abstract] ( Exit NIEHS
  15. Beard, W.A., Osheroff, W.P., Prasad, R., Jaju, M., Sawaya, M.R., Wood, T.G., Kraut, J., Kunkel, T.A., and Wilson, S.H. Enzyme-DNA interactions required for efficient nucleotide incorporation and discrimination in human DNA polymerase β. J. Biol. Chem., 271:12141-12144, 1996.  [Abstract] ( Exit NIEHS
  16. Beard, W.A., Minnick, D., Wade, C., Prasad, R., Won, R.L., Kumar, A., Kunkel, T.A., and Wilson, S.H. Role of the "Helix Clamp" in HIV-1 reverse transcriptase catalytic cycling as revealed by alanine-scanning mutagenesis. J. Biol. Chem., 271: 12213-12220, 1996.  [Abstract] ( Exit NIEHS
  17. Liu, D., Prasad, R., Wilson, S.H., DeRose, E.F., and Mullen, G.P. Three-dimensional solution structure of the N-terminal domain of DNA polymerase β and mapping of the ssDNA interaction interface. Biochemistry, 35:6188-6200, 1996.  [Abstract] ( Exit NIEHS
  18. Chyan, Y.-J., Strauss, P.R., Wood, T.G., and Wilson, S.H. Identification of novel mRNA isoforms for human DNA polymerase β. DNA and Cell Biology, 15:653-659, 1996.  [Abstract] ( Exit NIEHS
  19. Oda, N., Saxena, J.K., Jenkins, T.M., Prasad, R., Wilson, S.H., and Ackerman, E.J. DNA polymerases β and β are required for DNA repair in an efficient nuclear extract from Xenopus oocytes. J. Biol. Chem., 271:13816-13820, 1996.  [Abstract] ( Exit NIEHS
  20. Prasad, R., Singhal, R.K., Srivastava, D.K., Tomkinson, A.E., and Wilson, S.H. Specific interaction of DNA polymerase β and DNA ligase I in a multiprotein base excision repair complex from bovine testis. J. Biol. Chem., 271:16000-16007, 1996.  [Abstract] ( Exit NIEHS
  21. Piersen, C.E., Prasad, R., Wilson, S.H., and Lloyd, R.S. Evidence for an imino intermediate in the DNA polymerase β deoxyribose phosphate excision reaction. J. Biol. Chem., 271:17811-17815, 1996.  [Abstract] ( Exit NIEHS
  22. Narayan, S., He, F., and Wilson, S.H. Activation of the human DNA polymerase β promoter by a DNA-alkylating agent through induced phosphorylation of CREB-1. J. Biol. Chem., 271:18508-18513, 1996.  [Abstract] ( Exit NIEHS
  23. Lavrik, O.I., Prasad, R., Beard, W.A., Safronov, I.V., Dobrikov, M.I., Srivastava, D.K., Shishkin, G.V., Wood, T.G., and Wilson, S.H. dNTP binding to HIV-1 reverse transcriptase and mammalian DNA polymerase β as revealed by affinity labeling with a photoreactive dNTP analog. J. Biol. Chem., 271:21891-21897, 1996.  [Abstract] ( Exit NIEHS
  24. Pelletier, H., Sawaya, M.R., Wolfle, W., Wilson, S.H., and Kraut, J. Crystal structures of human DNA polymerase β complexed with DNA: Implications for catalytic mechanism, processivity, and fidelity. Biochemistry, 35:12742-12761, 1996.  [Abstract] ( Exit NIEHS
  25. Pelletier, H., Sawaya, M.R., Wolfle, W., Wilson, S.H., and Kraut, J. A structural basis for metal ion mutagenicity and nucleotide selectivity in human DNA polymerase β. Biochemistry, 35:12762-12777, 1996.  [Abstract] ( Exit NIEHS
  26. Reha-Krantz, L.J., Nonay, R.L., Day III, R.S., and Wilson, S.H. Replication of O6-Methylguanine-containing DNA by repair and replicative DNA polymerases. J. Biol. Chem., 271:20088-20095, 1996.  [Abstract] ( Exit NIEHS
  27. Mullen, G.P. and Wilson, S.H. Repair activity in DNA polymerases: a structurally conserved helix-hairpin-helix motif in base excision repair enzymes and in DNA polymerase β. IN: Hickson, I.D. (ed), Base Excision Repair of DNA Damage. Landes Bioscience, 1997, pp. 121-135.
  28. Strauss, P.R., Beard, W.A., Patterson, T.A., and Wilson, S.H. Substrate binding by human apurinic/apyrimidinic endonuclease indicates a Briggs-Haldane Mechanism. J. Biol. Chem., 272:1302-1307, 1997.  [Abstract] ( Exit NIEHS
  29. Efrati, E., Tocco, G., Eritja, R., Wilson, S.H., and Goodman, M.F. Abasic translesion synthesis by DNA polymerase β violates the "A-Rule": Novel types of nucleotide incorporation by human DNA polymerase β at an abasic lesion in different sequence contexts. J. Biol. Chem., 272:2559-2569, 1997.  [Abstract] ( Exit NIEHS
  30. Forgacs, E., Latham, G., Beard, W.A., Prasad, R., Bebenek, K., Kunkel, T.A., Wilson, S.H., and Lloyd, R.S. Probing structure/function relationships of HIV-1 reverse transcriptase with styrene oxide N2-Guanine adducts. J. Biol. Chem., 272:8525-8530, 1997.  [Abstract] ( Exit NIEHS
  31. Kim, S., Merrill, B.M., Rajpurohit, R., Kumar, A., Stone, K.L., Papov, V.V., Schneidersm J.M., Szer, W., Wilson, S.H., Paik, W.K., and Williams, K.R. Identification of NG-Methylarginine residues in human heterogeneous RNP protein A1: Phe/Gly-Gly-Gly-Arg-Gly-Gly-Gly/Phe is a preferred recognition motif. Biochemistry, 36:5185-5192, 1997.  [Abstract] ( Exit NIEHS
  32. Mullen, G.P. and Wilson, S.H. DNA polymerase β in abasic site repair: a structurally conserved helix-hairpin-helix motif in lesion detection by base excision repair enzymes. Biochemistry, 36:4713-4717, 1997.  [Abstract] ( Exit NIEHS
  33. Bebenek, K., Beard, W.A., Darden, T.A., Li, L., Prasad, R., Luxon, B.A., Gorenstein, D.G., Wilson, S.H., and Kunkel, T.A. A minor groove binding track in reverse transcriptase. Nat. Struct. Biol., 4:194-197, 1997.  [Abstract] ( Exit NIEHS
  34. Butler, A.P., Johnson, D.G., Kumar, A.P., Narayan, S., Wilson, S.H., and MacLeod, M.C. Disruption of transcription in vitro and gene expression in vivo by DNA adducts derived from a benzo[a]pyrene diol epoxide located in heterologous sequences. Carcinogenesis, 18:239-244, 1997.  [Abstract] ( Exit NIEHS
  35. Mullen, G.P., Antuch, W., Maciejewski, M.W., Prasad, R., and Wilson, S.H. Insights into the mechanism of the β-elimination catalyzed by the N-terminal domain of DNA polymerase β. Tetrahedron, 53:12057-12066, 1997.
  36. Sawaya, M.R., Prasad, R., Wilson, S.H., Kraut, J., and Pelletier, H. Crystal structures of human DNA polymerase β complexed with gapped and nicked DNA: evidence for an induced fit mechanism. Biochemistry, 36:11205-11215, 1997.  [Abstract] ( Exit NIEHS
  37. Yang, X-P., He, F., Rawson, T.Y., and Wilson, S.H. Human DNA polymerase β promoter: Phorbol ester activation is mediated through the cAMP response element and cAMP-response-element-binding protein. J. Biomed. Sci., 4:279-288, 1997.  [Abstract] ( Exit NIEHS
  38. Wilson, S.H., Sobol, R.W., Beard, W.A., Horton, J.K., Prasad, R., and Vande Berg, B.J. DNA polymerase-β and mammalian base excision repair. IN: Cold Spring Harbor Symposia on Quantitative Biology, Cold Spring Harbor Laboratory Press, 2000, Vol. 65, pp. 143-155.  [Abstract] ( Exit NIEHS
  39. Beard, W.A., Shock, D.D., Yang, X.-P., DeLauder, S.F., and Wilson, S.H. Loss of DNA polymerase β stacking interactions with templating purines, but not pyrimidines, alters catalytic efficiency and fidelity. J. Biol. Chem., 277:8235-8242, 2002.  [Abstract] ( Exit NIEHS
  40. Beard, W.A., Shock, D.D., Vande Berg, B.J., and Wilson, S.H. Efficiency of correct nucleotide insertion governs DNA polymerase fidelity. J. Biol. Chem., 277:47393-47398, 2002.  [Abstract] ( Exit NIEHS
  41. Beard, W.A., and Wilson, S.H. Structural insights into the origins of DNA polymerase fidelity. Structure (Camb.), 11:489-496, 2003.  [Abstract] ( Exit NIEHS
  42. Horton, J.K., Stefanick, D.F., Naron, J.M., Kedar, P.S., and Wilson, S.H. Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest following DNA methylating agent exposure. J. Biol. Chem., 280:15773-15785, 2005.  [Abstract] ( Exit NIEHS
  43. Batra, V.K., Beard, W.A., Shock, D.D., Pedersen, L.C., and Wilson, S.H. Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate. Structure (Camb.), 13:1225-1233, 2005.  [Abstract] ( Exit NIEHS
  44. Prasad, R., Batra, V.K., Yang, X.-P., Krahn, J.M., Pedersen, L.C., Beard, W.A., and Wilson, S.H. Structural insight into the DNA polymerase β deoxyribose phosphate lyase mechanism. DNA Repair (Amst.), 4:1347-1357, 2005.  [Abstract] ( Exit NIEHS
  45. Kirby, T.W., DeRose, E.F., Beard, W.A., Wilson, S.H., and London, R.E. A thymine isostere in the templating position disrupts assembly of the closed DNA polymerase β ternary complex. Biochemistry, 44:15230-15237, 2005.  [Abstract] ( Exit NIEHS
  46. Beard, W.A. and Wilson, S.H. Structure and mechanism of DNA polymerase β. Chemical Reviews, "DNA Damage and Repair," 106:361-382, 2006.  [Abstract] ( Exit NIEHS
  47. Batra, V.K., Beard, W.A., Shock, D.D., Krahn, J.M., Pedersen, L.C., and Wilson, S.H. Magnesium-induced assembly of a complete DNA polymerase catalytic complex. Structure (Camb.), 14:757-766, 2006.  [Abstract] ( Exit NIEHS
  48. Yoshimura, M., Kohzaki, M., Nakamura, J., Asagoshi, K., Sonoda, E., Hou, E., Prasad, R., Wilson, S.H., Tano, K., Yasui, A., Lan, L., Seki, M., Wood, R.D., Arakawa, H. Buerstedde, J.-M., Hochegger, H., Okada, T., Hiraoka, M., and Takeda, S. Vertebrate PolQ and Polβ cooperate in base excision repair of oxidative DNA damage. Mol. Cell, 24:115-125, 2006.  [Abstract] ( Exit NIEHS
  49. Lin, P., Pedersen, L.C., Batra, V.K., Beard, W.A., Wilson, S.H., and Pedersen, L.G. Energy analysis of chemistry for correct nucleotide insertion by DNA polymerase β. Proc. Natl. Acad. Sci. USA, 103:13294-13299, 2006.  [Abstract] ( Exit NIEHS
  50. Batra, V.K., Shock, D.D., Prasad, R., Beard, W.A., Hou, E.W., Pedersen, L.C., Sayer, J.M., Yagi, H., Kumar, S., Jerina, D.M., and Wilson, S.H. Structure of DNA polymerase β with a benzo[c]phenanthrene diol epoxide-adducted template exhibits mutagenic features. Proc. Natl. Acad. Sci. USA, 103:17231-17236, 2006.  [Abstract] ( Exit NIEHS
  51. Radhakrishnan, R., Arora, K., Wang, Y., Beard, W.A., Wilson, S.H., and Schlick, T. Regulation of DNA repair fidelity by molecular checkpoints: "Gates" in DNA polymerase β’s substrate selection. Biochemistry, 45:15142-15156, 2006.  [Abstract] ( Exit NIEHS
  52. Horton, J.K., Stefanick, D.F., Kedar, P.S., and Wilson, S.H. ATR signaling mediates an S-phase checkpoint after inhibition of poly(ADP-ribose) polymerase activity. DNA Repair (Amst.).  [Abstract] ( Exit NIEHS
  53. Wang Y, Reddy S, Beard WA, Wilson SH, Schlick T. Differing conformational pathways before and after chemistry for insertion of dATP vs. dCTP opposite 8-oxoG in DNA polymerase beta. Biophysical journal 9: 3063-3070, 2007. [Abstract] ( Exit NIEHS
  54. Das S, Chattopadhyay R, Bhakat KK, Boldogh I, Kohno K, Prasad R, Wilson SH, Hazra TK . Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress. The Journal of biological chemistry 282(39):28474-28484, 2007. [Abstract] ( Exit NIEHS
  55. Moon AF, Garcia-Diaz M, Batra VK, Beard WA, Bebenek K, Kunkel TA, Wilson SH, Pedersen LC. The X family portrait: structural insights into biological functions of X family polymerases. DNA repair 6(12):1709-1725, 2007. [Abstract] ( Exit NIEHS
  56. Kovtun IV, Liu Y, Bjoras M, Klungland A, Wilson SH, McMurray CT. OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells. Nature 447(7143):447-452, 2007. [Abstract] ( Exit NIEHS
  57. Liu Y, Prasad R, Beard WA, Kedar PS, Hou EW, Shock DD, Wilson SH. Coordination of steps in single-nucleotide base excision repair mediated by apurinic/apyrimidinic endonuclease 1 and DNA polymerase β. The Journal of biological chemistry 282(18):13532-13541, 2007. [Abstract] ( Exit NIEHS
  58. Nakanishi S, Prasad R, Wilson SH, Smerdon M. Different structural states in oligonucleosomes are required for early versus late steps of base excision repair.. Nucleic Acids Research 35(13):4313-4321, 2007. [Abstract] ( Exit NIEHS
  59. Palma N, Cinelli S, Sapora O, Wilson SH, Dogliotti E. Ochratoxin A-induced mutagenesis in mammalian cells is consistent with production of oxidative stress.. Chemical research in toxicology 20(7):1031-1037 2007. [Abstract] ( Exit NIEHS
  60. Horton JK, Stefanick DF, Kedar PS, Wilson SH. ATR signaling mediates an S-phase checkpoint after inhibition of poly(ADP-ribose) polymerase activity. DNA repair 6(6):742-750, 2007. [Abstract] ( Exit NIEHS
  61. Tano K, Nakamura J, Asagoshi K, Arakawa H, Sonoda E, Braithwaite EK, Prasad R, Buerstedde J-M, Takeda S, Watanabe M, Wilson SH. Interplay between DNA polymerases beta and lambda in repair of oxidation DNA damage in chicken DT40 cells. DNA repair 6(6):869-875, 2007. [Abstract] ( Exit NIEHS
  62. Oelschlaeger P, Klahn M, Beard WA, Wilson SH, Warshel A. Magnesium-cationic dummy atom molecules enhance representation of DNA polymerase beta in molecular dynamics simulations: Improved accuracy in studies of structural features and mutational effects. Journal of molecular biology 366(2):687-701, 2007. [Abstract] ( Exit NIEHS
  63. Hou EW, Prasad R, Asagoshi K, Masaoka A, Wilson SH. Comparative assessment of plasmid and oligonucleotide DNA substrates in measurement of in vitro base excision repair activity. Nucleic acids research 35(17):e112, 2007. [Abstract] ( Exit NIEHS
  64. McKenna CE, Kashemirov BA, Upton TG, Batra VK, Goodman MF, Pedersen LC, Beard WA, Wilson SH . (R)-beta,gamma-fluoromethylene-dGTP-DNA ternary complex with DNA polymerase beta. Journal of the American Chemical Society 129(50):15412-15413, 2007. [Abstract] ( Exit NIEHS
  65. Prasad R, Liu Y, Deterding LJ, Poltoratsky VP, Kedar PS, Horton JK, Kanno SI, Asagoshi K, Hou EW, Khodyreva SN, Lavrik OI, Tomer KB, Yasui A, Wilson SH. HMGB1 is a co-factor in mammalian base excision repair. Molecular cell 27(5):829-841, 2007. [Abstract] ( Exit NIEHS
  66. Horton JK, Watson M, Stefanick DF, Shaughnessy DT, Taylor JA, Wilson SH. XRCC1 and DNA polymerase beta in cellular protection against cytotoxic DNA single-strand breaks. Cell research 18(1):48-63, 2008. [Abstract] ( Exit NIEHS
  67. Xiang Y, Goodman MF, Beard WA, Wilson SH, Warshel A. Exploring the role of large conformational changes in the fidelity of DNA Polymerase beta. Proteins 70(1):231-247, 2008. [Abstract] ( Exit NIEHS
  68. Batra VK, Beard WA, Shock DD, Pedersen LC, Wilson SH. Structures of DNA polymerase beta with active site mismatches suggest a transient abasic site intermediate during misincorporation. Molecular cell 30(3):315-324, 2008. [Abstract] ( Exit NIEHS
  69. Kronenberg G, Harms C, Sobol RW, Cardozo-Pelaez F, Linhart H, Winter B, Balkaya M, Gertz K, Gay SB, Cox D, Eckart S, Ahmadi M, Juckel G, Kempermann G, Hellweg R, Sorh R, Hortnagl H, Wilson SH, Jaenisch R, Endres M. Folate deficiency induces neurodegeneration and brain dysfunction in mice lacking uracil DNA glycosylase.. The Journal of neuroscience : the official journal of the Society for Neuroscience 28(28):7219-7230, 2008. [Abstract] ( Exit NIEHS
  70. Kedar PS, Stefanick DF, Horton JK, Wilson SH. Interaction between PARP-1 and ATR in mouse fibroblasts is blocked by PARP inhibition. DNA repair 7(11):1787-1798, 2008. [Abstract] ( Exit NIEHS
  71. Allen D, Herbert DC, McMahan CA, Rotrekl V, Sobol RW, Wilson SH, Walter CA. Mutagenesis is elevated in male germs cells obtained from DNA polymerase-beta heterozygous mice. Biology of reproduction 79(5):824-831, 2008. [Abstract] ( Exit NIEHS
  72. Poltoratsky VP, Horton, JK, Prasad, R, Beard, WA, Woodgate, R, Wilson, SH. Negligible impact of pol iota expression on the alkylation sensitivity of pol beta-deficient mouse fibroblast cells. DNA repair 7(6):830-833, 2008. [Abstract] ( Exit NIEHS

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