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Proteomics in Diabetes

Nather Conference Center
National Institute of Health
Bethesda, Maryland
April 24-25, 2003

Summary

High throughput DNA sequencing has changed biomedical research. The complete sequence of the genome for 100 organisms and the nearly complete sequence of many others, including the human, are giving a new vision to the study of biological systems. It is, however, apparent that knowledge of the genome alone is not sufficient for a complete understanding of complex biological processes. While the genome is the same in different cell types within an organism and does not change with time, the proteome varies with time and among different cell types. Cell function is usually fulfilled through its proteins whereas its potential is indicated by the genome. In view of recent large-scale data showing that often there is a poor correlation between mRNA quantities and protein quantities, we cannot limit gene expression studies to the RNA level but must also investigate biological problems at the protein level. Despite the significant limitations of using mRNA arrays for studying gene expression, relatively few investigators have used proteomic approaches. Moreover, post-translational modifications, regulation of protein function by proteolysis, and composition of macromolecular complexes or organelles can only be determined at the protein level, further emphasizing the importance of studying the proteome.

Proteomic approaches have been successfully used for studying complex biological problems and for the identification of disease markers. Recent developments in proteomics indicate that the technologies available are already sufficiently advanced to approach many biological questions relevant to the NIDDK mandate.

This workshop will provide a venue to bring together investigators with expertise in proteomics and those interested in applying this technology to problems related to diabetes, endocrinology and metabolic diseases. Several leaders in the field will illustrate the state of the art in proteomics and their possible use to study diabetes

Agenda

8:30 a.m. Meeting Opening
Salvatore Sechi, Director Proteomic Program
 
8:35 a.m.  Welcome and Introduction
Allen Spiegel, Director NIDDK

8:45 a.m.  KEYNOTE LECTURE
Dissecting the Nutrient-Response Signaling Network Using Chemical Genetics

Stuart L. Schreiber, Harvard University
BIO  http://www-schreiber.chem.harvard.edu
http://iccb.med.harvard.edu/
http://www.cgr.harvard.edu/

Protein Profiling and Quantitation (35 minute presentations, ~5 minutes for questions)

9:30 a.m.  Quantitative Proteomics: New Technologies and Applications
Ruedi Aebersold, The Institute for Systems Biology
BIO: http://www.systemsbiology.org/Default.aspx?pagename=ruediaebersold

10:10 a.m. Break (20 minutes)

10:30 a.m. Functional Proteomic Analysis of Signal Transduction and Disease
Natalie Ahn, University of Colorado
BIO: http://www.colorado.edu/chemistry/faculty/Ahn/

11:10 a.m.  Proteomics Methods to Elucidate Diabetes Related Signaling
Matthias Mann, University of Southern Denmark - Odense University
BIO: http://www.pil.sdu.dk/matthias_mann.htm

11:50 a.m. New Technologies for Comprehensive, Quantitative and High Throughput Proteomics
Richard D. Smith, Pacific Northwest National Laboratory (PNNL
BIO: http://emslbios.pnl.gov/bios/biosketch.nsf/All+Public+Documents/Smith,+Richard+D?Opendocument

12:10 p.m. Computational Tools for High Throughput Proteomics
Andy Keller, The Institute for Systems Biology
http://www.systemsbiology.org

12:30 p.m.  Lunch (on your own)

Protein Profiling and Quantitation
(35 minute presentations, ~5 minutes for questions)

Adipose Tissue-Insulin Receptor Knockout Unmasks Heterogeneity of Protein and Gene Expression Patterns Depending on the Adipocyte Size
C. Ronald Kahn, Joslin Diabetes Center
BIO: http://www.joslindiabetescenter.org/news/new_president.shtml

2:10 p.m. Application of the Serum Proteome in Metabolic Diseases
Christian Rholff, Oxford GlycoSciences, UK
BIO: http://www.ogs.com

2:50 p.m.  On the Pathogenesis of Type 1 Diabetes, Proteomic Studies
Jorn Nerup, Steno Diabetes Center, Gentofte, Denmark

3:30 p.m. Break (20 minutes)

3:50 p.m. Cells and Organelles in Diabetes:
A Combined Genomic and Proteomic Approach
Silvia Corvera,University of Massachusetts Medical School
BIO: http://www.umassmed.edu/igp/faculty/corvera.cfm?start=Research&name=corvera

4:30 p.m. Presentation on Hupo Proteomics Initiatives
Samir Hanash , University of Michigan
http://hupo.org

4:50 p.m. Poster Viewing and Refreshments

5:50 p.m. Adjournment
April 25, 2003

Protein Interaction and Computational Modeling
(35 minute presentations, ~5 minutes for questions)

8:30 a.m.    Integrating “Omic” Information:
A Bridge Between Genomics and Systems Biology
Marc Vidal, Harvard Medical School
BIO: http://vidal.dfci.harvard.edu/main_pages/homepage.html


9:10 a.m.  Accelerating Disease Gene Discovery through Integrative Genomics
Vamsi Mootha, Whitehead Institute / MIT
BIO:http://www-genome.wi.mit.edu/mpg/people/vmootha.html

Talks Selected From Posters
(10 minute presentations, ~5 minutes for questions)

9:50 a.m. Insulin Resistance and O-GlcNAc Modification of Nucleocytoplasmic Proteins
Lance Wells*, Stephen A. Whelan*, Keith Vosseller, and Gerald W. Hart
Department of Biological Chemistry,Johns Hopkins School of Medicine, Baltimore,MD

10:05 a.m.  Proteomics on Secretory Proteins from Adipose Cells
Xiaoli Chen¹, Sonja Hess², Lewis Pannell² and Samuel W. Cushman^1
1 EDMNS/DB and ²Structural Mass Spectrometry Facility, NIDDK, NIH, Bethesda, MD

10:20 a.m.  Protein Profiling of Nuclear Proteins Regulated by EDG-1, a High Affinity G-Protein Coupled Receptor for Sphingosine-1-phosphate
Menq-Jer Lee, Sung-Suk Chae, Nicollas Ancellin, Timothy Hla, David Han
Center for Vascular Biology/ Department of Physiology
University of Connecticut Health Center, Farmington, CT

10:35 a.m.  Break (25 minutes)

Protein Interaction and Computational Modeling (Continued
(35 minute presentations, ~5 minutes for questions)

11:00 a.m.  The Biomolecular Interaction Network Database BR>Christopher Hogue , University of Toronto, Canada BIO: http://bioinfo.mshri.on.ca/index.html

11:40 a.m.  New Proteins in Insulin Signaling and Action
Gus Lienhard, Dartmouth Medical School
BIO: http://www.dartmouth.edu/~biochem/lienhard/people.html#PI

12:30 p.m. Lunch on your own

Target Discovery and Protein Function
(35 minute presentations, ~5 minutes for questions

1:30 p.m.  DNA as a Pharmaceutical: Large Scale Proteomic Identification of Targets in Autoimmune Disease Allows Tolerization by Novel DNA Constructs
Larry Steinman, Stanford University School of Medicine
BIO: http://www.stanford.edu/group/neurology/fac/steinman.html

2:10 p.m.  Differential Proteomic Analysis in Animal Models of Type 2 Diabetes and Obesity-A Search for New Molecular Targets
Mike Cawthorne, The University of Buckingham, UK
BIO: http://www.buckingham.ac.uk/clore/

2:50 p.m.  Global Analysis of Biochemical Activities Using Protein Chips
Mike Snyder, Yale University
BIO: http://www.yale.edu/snyder/

3:20 p.m.  Meeting Adjourns

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