Product Pathways - Translational Control
Phospho-GCN2 (Thr898) Antibody #3301
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 220 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 3301:
- Western Blotting
Specificity / Sensitivity
Phospho-GCN2 (Thr898) Antibody detects endogenous levels of GCN2 only when phosphorylated at threonine 898.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr898 of murine GCN2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from SV-T2 cells, untreated or UV-treated (50 mJ/cm2 for 30 minutes), using Phospho-GCN2 (Thr898) Antibody.
Background
Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (1,2). GCN2 is also required for UV-light induced translation inhibition, and in vivo phosphorylation of murine GCN2 at Thr898 is induced by both UV irradiation and by leucine deprivation (3). UV-induced activation of NF-kappaB also requires GCN2, which may act simply by preventing translation of IkappaB-alpha to replace pools that have been ubiquitinated and degraded (4). Interestingly, proteasome inhibitors (MG132 and ALLN) activate the GCN2/eIF2alpha pathway, suggesting a pivotal role for this kinase in stress response and ubiquitin-mediated signaling (5). In vitro autophosphorylation of yeast GCN2 within its activation loop at Thr882 and Thr887 (Thr898 and Thr903 in mouse) has also been reported (6).
- Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
- Sheikh, M.S. and Fornace, A.J. (1999) Oncogene 18, 6121-6128.
- Deng, J. et al. (2002) Curr. Biol. 12, 1279-1286.
- Jiang, H.Y. and Wek, R.C. (2005) Biochem. J. 385, 371-380.
- Jiang, H.Y. and Wek, R.C. (2005) J. Biol. Chem. XXX, XXX-XXX.
- Garcia-Barrio, M. et al. (2002) J. Biol. Chem. 277, 30675-30683.
Application References
- Deng, J. et al. (2002) Activation of GCN2 in UV-irradiated cells inhibits translation. Curr Biol. 12, 1279-1286. This article references the use of Phospho-GCN2 (Thr898) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3302 GCN2 Antibody
- 3597 Phospho-eIF2α (Ser51) (119A11) Rabbit mAb
- 9721 Phospho-eIF2α (Ser51) Antibody
- 9722 eIF2α Antibody
- 3034 NF-κB p65 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
