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WB: Acid extracts from untreated (Lane 1) and colcemid-arrested (Lane 2) HeLa cells were probed with anti-phospho-CENP-A (Ser7).
Anti-phospho-CENP-A (Ser7)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P49450 # Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
SIZE: 140 amino acids; 15991 Da
SUBUNIT: Forms a nucleosome-like h ... see more »
SIZE: 140 amino acids; 15991 Da
SUBUNIT: Forms a nucleosome-like h ... see more »
Entrez Gene Number:
Entrez Gene Summary
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multipl
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Description:
Anti-phospho-CENP-A (Ser7)
Specificity:
phosphorylated CENP-A (Ser7)
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Mouse
Isotype:
IgG
Immunogen:
peptide contianing the sequence RRpSRK in which pS is phosphoserine corresponding to amino acid 7 of human CENP-A (centromere protein A)
Modifications:
Phosphorylation
Molecular Weight:
~17kDa
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
Gene Symbol:
- CENPA
- CENP-A
Quality Assurance:
routinely evaluated by immunoblot on acid extracted proteins from colcemid-arrested HeLa cells
Antibody Category:
Nuclear Function
Antibody Sub-Category:
Cell Cycle, DNA Replication & Repair
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
CENP-A is a specialized homolog of histone H3 found in discrete kinetochore-forming domains that folds in mitotic chromosomes to form the inner kinetochore plate. Phosphorylation of CENP-A on Ser 7 occurs very late in G2 as cells prepare to enter mitosis.
Trade Name:
Upstate (Millipore)
Purification Method:
protein A purified
Format:
Purified
Product Overview:
CENP-A is a variant version of histone H3 found only at centromeres. It is phosphorylated at serine 7 during mitosis.
Antibody Type:
Polyclonal Antibody
Host:
Rabbit