| QUALITY CONTROL STEPS DESCRIPTION |
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Quality Control Description Type(s):
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technical_replicate
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Description:
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To validate the array data, semiquantitative RT¿PCR (sqRT¿PCR) analysis of several messenger RNAs (mRNAs) was performed in a representative subset of tumors containing five BRCA1-linked RNA samples, five BRCA2-linked samples, and five sporadic samples. Expression of TOP2A, RGS1, CD74, HE4, HLA-DRB1, and ZFP36 genes was evaluated by sqRT¿PCR with -actin as a normalizing control. Because data obtained from cDNA microarrays are in the form of relative expression ratios between tumors and the reference sample, we also included an IOSE cell RNA sample and a normal ovarian RNA sample in the sqRT¿PCR experiments for comparison. The results of these sqRT¿PCR experiments were consistent with the cDNA microarray relative expression data for all six genes evaluated
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