Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-Src Family (Tyr416) (100F9) Rabbit mAb #2113
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R (C) | Endogenous | 60 kDa | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
Species cross-reactivity is determined by Western blot.
Protocols
- 2113:
- Western Blotting
Specificity / Sensitivity
Phospho-Src Family (Tyr416) (100F9) Rabbit mAb detects endogenous levels of Src only when phosphorylated at tyrosine 416. The antibody may cross-react with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr416 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of Colo201 cells serum starved and stimulated with 20% FBS for 5 min, using Phospho-Src family (Tyr416) (100F2) Rabbit mAb (upper) and Src (L4A1) Mouse mAb #2110 (lower).
Background
The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
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Companion Products
- 2109 Src (36D10) Rabbit mAb
- 2101 Phospho-Src Family (Tyr416) Antibody
- 2102 Non-phospho-Src (Tyr416) (7G9) Mouse mAb
- 2105 Phospho-Src (Tyr527) Antibody
- 2107 Non-phospho-Src (Tyr527) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2108 Src Antibody
- 2110 Src (L4A1) Mouse mAb
- 2123 Src (32G6) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
