Product Pathways - MAPK Signaling
Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211
Applications | Reactivity | Sensitivity | MW (kDa) | Source |
---|---|---|---|---|
W IP IF-IC F | H M R Mk Pg Sc Dm (Z) | Endogenous | 43 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Pg=Pig
Sc=S. cerevisiae
Dm=D. melanogaster
Z=Zebrafish
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Phospho-p38 MAPK (Thr180/Tyr182) Antibody detects endogenous levels of p38 MAPK only when activated by phosphorylation at threonine 180 and tyrosine 182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAPK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
![Western Blotting](images/9210_wb_jb_040707.jpg)
Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or p38 MAPK Antibody #9212 (lower).
Western Blotting
![Western Blotting](images/9211_fig3.jpg)
Western blot analysis of extracts from UV-treated NIH/3T3 cells, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or control p38 MAPK Antibody #9212 (lower).
Flow Cytometry
![Flow Cytometry](images/9211_fig5.jpg)
Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin treated (blue), using Phospho-p38 MAPK (Thr180/Tyr182) Antibody compared to a nonspecific negative control antibody (red).
Background
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
- Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
- Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
Application References
- Kawagoe, T. et al. (2008) Nat Immunol 9, 684-91. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Herczenik, E. et al. (2007) Arterioscler Thromb Vasc Biol 27, 1657-65. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Zaru, R. et al. (2007) Nat Immunol 8, 1227-35. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Hsu, J.T. et al. (2007) Am J Physiol Heart Circ Physiol 292, H2982-7. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Goss, V.L. et al. (2006) Blood 107, 4888-97. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Matsumoto, M. et al. (2004) J Biol Chem 279, 45969-79. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: IF-IC (In Cells)
- Patrucco, E. et al. (2004) PI3Kgamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects. Cell 118, 375-387. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Hata, K. et al. (2003) Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis. Mol. Biol. Cell 14, 545-555. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Liu, F. et al. (2002) GnRH activates ERK1/2 leading to the induction of c-fos and LHbeta protein expression in LbetaT2 cells. Mol. Endocrinol. 16, 419-434. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Xing, H. et al. (2000) 14-3-3 proteins block apoptosis and differentially regulate MAPK cascades. EMBO J. 19, 349-358. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Villunger, A. et al. (2000) Fas ligand, Bcl-2, granulocyte colony-stimulating factor, and p38 mitogen-activated protein kinase: regulators of distinct cell death and survival pathways in granulocytes. J. Exp. Med. 192, 647-658. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Lee, Y. W. et al. (2000) 2-Amino-3-methylimidazo[4,5-f] quinoline inhibits nitric oxide production in lipopolysaccharide-stimulated RAW 264. Cancer Lett. 156, 133-139. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
- Uddin, S. et al. (2000) The Rac1/p38 mitogen-activated protein kinase pathway is required for interferon alpha-dependent transcriptional activation but not serine phosphorylation of Stat proteins. J. Biol. Chem. 275, 27634-27640. This article references the use of Phospho-p38 MAPK (Thr180/Tyr182) Antibody in the following applications: Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.