Product Pathways - MAPK Signaling
Phospho-SEK1/MKK4 (Thr261) Antibody #9151
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 46 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 9151:
- Western Blotting
Specificity / Sensitivity
Phospho-SEK1/MKK4 (Thr261) Antibody detects endogenous levels of SEK1/MKK4 protein only when phosphorylated at threonine 261. This antibody does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH coupled) corresponding to residues around Thr261 of human SEK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from untreated and UV treated 3T3, C6 and 293 cells, using Phospho-SEK1/MKK4 (Thr261) Antibody.
Background
SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).
- Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
- Sanchez, I. et al. (1994) Nature 372, 794-798.
- Yan, M. et al. (1994) Nature 372, 798-800.
- Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
- Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.
Application References
- Wolfman, J. C. et al. (2002) Cellular N-Ras promotes cell survival by downregulation of Jun N-terminal protein kinase and p38. Molec. Cell. Biol. 22, 1589-1606. This article references the use of Phospho-SEK1/MKK4 (Thr261) Antibody in the following applications: Western Blotting
- Benhar, M. et al. (2001) Enhanced ROS production in oncogenically transformed cells potentiates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase activation and sensitization to genotoxic stress. Mol. Cell. Biol. 21 (20), 6913-6926. This article references the use of Phospho-SEK1/MKK4 (Thr261) Antibody in the following applications: Western Blotting
- Boppart, M. D. et al. (2000) Marathon running transiently increases c-Jun NH2-terminal kinase and p38 activities in human skeletal muscle. J. Cell. Physiol. 526, 663-669. This article references the use of Phospho-SEK1/MKK4 (Thr261) Antibody in the following applications: Western Blotting
- Bourbon, N. A. et al. (2000) Ceramide directly activates protein kinase C zeta to regulate a stress-activated protein kinase signaling complex. J. Biol. Chem. 275, 35617-35623. This article references the use of Phospho-SEK1/MKK4 (Thr261) Antibody in the following applications: Western Blotting
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Companion Products
- 3346 SEK1 (5C10) Rabbit mAb
- 4514 Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb
- 9152 SEK1/MKK4 Antibody
- 9155 Phospho-SEK1/MKK4 (Ser80) Antibody
- 9156 Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
