Product Pathways - MAPK Signaling
Phospho-MKK7 (Ser271/Thr275) Antibody #4171
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 48 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 4171:
- Western Blotting
Specificity / Sensitivity
Phospho-MKK7 (Ser171/Thr275) Antibody detects endogenous levels of MKK7 phosphorylated at serine 271 and threonine 275. This antibody does not cross-react with phosphorylated SEK1/MKK4 or other phosphorylated MAP kinase kinases.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser271/Thr275 of human MKK7. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis and extracts from THP1, U-937 and Ramos cells, untreated or sorbitol-treated, using Phospho-MKK7 (Ser271/Thr275) Antibody (upper) or MKK7 Antibody #4172 (lower).
Background
MKK7 is a MAP kinase kinase that serves as a specific activator of JNK/SAPK pathway (1,2). MKK7 is activated strongly by TNFalpha, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and JNK/SAPK pathways, is not activated by TNFalpha (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.
- Tournier, C. et al. (1999) Mol. Cell. Biol. 19, 1569-1581.
- Moriguchi, T. et al. (1997) EMBO J. 16, 7045-7053.
Application References
- Zapata, H.J. et al. (2007) J Virol 81, 977-90. This article references the use of Phospho-MKK7 (Ser271/Thr275) Antibody in the following applications: Western Blotting
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- 4172 MKK7 Antibody
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
