Product Pathways - Glucose Metabolism
Phospho-Glycogen Synthase (Ser641) Antibody #3891
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P | H M R | Endogenous | 85 to 90 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Phospho-Glycogen Synthase (Ser641) Antibody detects endogenous levels of both muscle and liver isoforms of glycogen synthase only when phosphorylated at serine 640 or 641 of the muscle and liver isoforms, respectively.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser641 of human liver glycogen synthase. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells treated with calf intestinal alkaline phosphatase (CIP), using Phospho-Glycogen Synthase (Ser641) Antibody (upper) or control glycogen synthase antibody (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-Glycogen Synthase (Ser641) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded renal clear cell adenocarcinoma using Phospho-Glycogen Synthase (Ser641) Antibody.
Background
Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).
Ser641 is one of the most important phosphorylation sites for the regulation of glycogen synthase (5).
- Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
- Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
- Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
- Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.
- Skurat, A.V. and Roach, P.J. (1995) J. Biol. Chem. 270, 12491-12497.
Application References
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Companion Products
- 3893 Glycogen Synthase Antibody
- 3886 Glycogen Synthase (15B1) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
