Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PLCγ2 (Tyr759) Antibody #3874

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M Endogenous 150 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Phospho-PLCgamma2 (Tyr759) Antibody detects endogenous levels of PLCgamma2 only when phosphorylated at tyrosine 759. The antibody does not cross-react with phosphorylated PLCgamma1.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr759 of human PLCgamma2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or anti-human IgM treated Ramos cells or lamda phosphatase-treated Ramos cell lysates, using Phospho-PLCgamma2 (Tyr759) Antibody (upper) or PLCgamma2 Antibody #3872 (lower).

Background

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3).Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6).PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).

PLCgamma2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at tyrosines 753, 759, 1197 and 1217 is correlated with PLCgamma2 activity (9,10).

  1. Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
  2. Smrcka, A. V. et al. (1991) Science 251, 804-807.
  3. Taylor, S. J. et al. (1991) Nature 350, 516-518.
  4. Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027.
  5. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
  6. Margolis, B. et al. (1989) Cell 57, 1101-1107.
  7. Kim, H. K. et al. (1991) Cell 65, 435-441.
  8. Wang, Z. et al. (1998) Mol. Cell. Biol. 18, 590-597.
  9. Watanabe, D. et al. (2001) J. Biol. Chem. 276, 38595-38601.
  10. Ozdener, F. et al. (2002) Mol. Pharmacol. 62, 672-679.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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