Product Pathways - Cell Cycle / Checkpoint
Phospho-Pin1 (Ser16) Antibody #3721
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | R (H) (M) (X) | Endogenous | 18 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
X=Xenopus
Species cross-reactivity is determined by Western blot.
Protocols
- 3721:
- Western Blotting
Specificity / Sensitivity
Phospho-Pin1 (Ser16) Antibody detects endogenous levels of Pin1 only when phosphorylated at serine 16.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser16 of human Pin1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from C6 cells, untreated or treated with 0.1uM calyculin A #9902, using Phospho-Pin1 (Ser16) Antibody (upper) or Pin1 Antibody #3722 (lower).
Background
Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).
Phosphorylation of pin1 at Ser16 affects its subcellular localization and the ability of its WW domain to interact with potential targets (10).
- Lu, P. J. et al. (1999) Science 283, 1325-1328.
- Verdecia, M. A. et al. (2000) Nat. Struct. Biol. 7, 639-643.
- Lu, K. P. et al. (1996) Nature 380, 544-547.
- Zhou, X. Z. et al. (2000) Mol. Cell 6, 873-883.
- Wu, X. et al. (2000) EMBO J. 19, 3727-3738.
- Winkler, K. E. et al. (2000) Science 287, 1644-1647.
- Crenshaw, D. G. et al. (1998) EMBO J. 17, 1315-1327.
- Shen, M. et al. (1998) Genes Dev. 12, 706-720.
- Yaffe, M. B. et al. (1997) Science 278, 1957-1960.
- Lu, P. J. et al. (2002) J Biol Chem 277, 2381-2384.
Application References
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Companion Products
- 3722 Pin1 Antibody
- 9527 Phospho-cdc25C (Thr48) Antibody
- 9528 Phospho-cdc25C (Ser216) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
