Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb #2865
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 135 (c-Abl); 210 (Bcr-Abl) | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
- 2865:
- Western Blotting
Specificity / Sensitivity
Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb detects endogenous Abl proteins only when phosphorylated at tyrosine 412. The antibody does not cross-react with other phosphorylated tyrosine kinases.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr412 of human c-Abl.
Western Blotting
Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins, using Phospho-c-Abl (Tyr412) (247C7 ) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). Phospho-c-Abl (Tyr412) (247C7 ) Rabbit mAb specifically recognizes the activated Abl of Bcr-Abl fusion proteins, whose phosphorylation was abolished by calf intestinal phosphatase (CIP) treatment.
Western Blotting
Western blot analysis of extracts from K562 cells, untreated or treated with an Abl inhibitor, using Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb.
Background
The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).
- Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
- Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
- Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
- Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
- Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
- Pluk, H. et al. (2002) Cell 108, 247-259.
Application References
- Goss, V.L. et al. (2006) Blood 107, 4888-97. This article references the use of Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2868 Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb
- 3009 Phospho-c-Abl (Tyr204) (C42B5) Rabbit mAb
- 2861 Phospho-c-Abl (Tyr245) Antibody
- 2862 c-Abl Antibody
- 2864 Phospho-c-Abl (Thr735) Antibody
- 3098 Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
