Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Phospho-LRP6 (Ser1490) Antibody #2568

Applications Reactivity Sensitivity MW (kDa) Source
W IP H (M) Endogenous 180, 210 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Phospho-LRP6 (Ser1490) Antibody detects endogenous levels of LRP6 protein when phosphorylated at serine 1490. It is expected, although not yet confirmed, to also detect endogenous levels of LRP5 protein when phosphorylated at serine 1493 (corresponding to serine 1490 in LRP6).

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser1490 of human LRP6. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HeLa cells untreated, treated 5 hours with conditioned medium (CM) from L cells, or treated 5 hours with conditioned medium from L cells overexpressing Wnt3a, using Phospho-LRP6 (Ser1490) Antibody #2568.

Background

LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density-lipoprotein (LDLR) receptor related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/beta-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by binding of some members of Dickkopf (DKK) family proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490 and Thr1493 by kinases such as GSK-3 and CK1 (8-10). The phosphorylated LRP6 recruits Axin to the membrane, and presumably activates beta-catenin signaling (8-10).

  1. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248, 879-888.
  2. Pinson, K.I. et al. (2000) Nature 407, 535-538.
  3. Tamai, K. et al. (2000) Nature 407, 530-535.
  4. Kelly, O.G. et al. (2004) Development 131, 2803-2815.
  5. He, X. et al. (2004) Development 131, 1663-1677.
  6. Bean, W.J. et al. (1975) Radiology 115, 485-486.
  7. Bafico, A. et al. (2001) Nat. Cell Biol. 3, 683-668.
  8. Tamai, K. et al. (2004) Mol. Cell 13, 149-156.
  9. Zeng, X. et al. (2005) Nature 438, 873-877.
  10. Davidson, G. et al. (2005) Nature 438, 867-872.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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