Ordering Information
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WB: FKHR fusion protein (lane 1) and FKHR fusion protein incubated with Akt1/PKBα (lane 2) were probed with anti-phospho-FKHR (Ser329).
Anti-phospho-FKHR (Ser329)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q12778 # Transcription factor.
SIZE: 655 amino acids; 69662 Da
SUBUNIT: Interacts with LRPPRC (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Thr-24, Ser-256 and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding a ... see more »
SIZE: 655 amino acids; 69662 Da
SUBUNIT: Interacts with LRPPRC (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Thr-24, Ser-256 and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding a ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.
Description:
Anti-phospho-FKHR (Ser329)
Specificity:
GST-FKHR phosphorylated on serine 329
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
Isotype:
IgG
Immunogen:
peptide corresponding to amino acids surrounding phosphorylated Ser329 of human FKHR
Modifications:
Phosphorylation
Molecular Weight:
70 - 80 kDa
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C
Gene Symbol:
- FOXO1
- FKHR
- FKH1
- FOXO1A
Quality Assurance:
routinely evaluated by immunoblot on FKHR fusion protein (Catalog #14-343) phosphorylated with active Akt1/PKBα (Catalog #14-276)
Antibody Category:
Nuclear Function
Antibody Sub-Category:
Transcription Factors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
The forkhead-related transcription factor stimulates transcription of pro-apoptotic genes such as Fas ligand. In growing cells, FKHRL1 is phosphorylated by Akt, and associates with 14-3-3 proteins. This association is sufficient to retain FKHRL1 in the cytoplasm, silencing its transcriptional activity. Other forkhead family members are involved in insulin response, pulmonary ciliogenesis, thyroid function, and numerous developmental events including the defect in nude mice.
Trade Name:
Upstate (Millipore)
Purification Method:
immunoaffinity purified
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit