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IB: Recombinant MKK7β1, unactive (14-542) was left untreated (Lane 1) or phosphorylated by MEKK1 (14-196) (Lane 2) and probed with anti-phospho-MKK7/ ...
Anti-phospho-MKK7/SKK4 (Thr275)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: O14733 # Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
COFACTOR: Magnesium.
SIZE: 419 amino acids; 47485 Da
SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity).
TISSUE SPECIFICITY: Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
PTM: Activate ... see more »
COFACTOR: Magnesium.
SIZE: 419 amino acids; 47485 Da
SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity).
TISSUE SPECIFICITY: Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
PTM: Activate ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced tr
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Description:
Anti-phospho-MKK7/SKK4 (Thr275)
Specificity:
MKK7/SKK4
Key Applications:
Western Blotting
Species Reactivity:
Human
Species Reactivity Note:
Cross-reactivity with mouse expected due to sequence homology
Isotype:
IgG
Immunogen:
peptide corresponding to amino acids surrounding phosphorylated threonine 275 of human MKK7/SKK4
Modifications:
Phosphorylation
Molecular Weight:
47kDa
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
Gene Symbol:
- MAP2K7
- MKK7
- PRKMK7
- MEK7
- MAPKK7
- JNKK2
- Jnkk2
Quality Assurance:
routinely evaluated by immunoblot on recombinant MKK7β1, unactive (14-542) that had been phosphorylated by MEKK1 (14-196)
Antibody Category:
Cell Signaling
Antibody Sub-Category:
MAP Kinases
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Trade Name:
Upstate (Millipore)
Purification Method:
protein A purified
Format:
Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit