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Kinase/WB Analysis: Recombinant CIP-17 was incubated in the presence of ATP with (lane 4) or without (lanes 1, 2.and 3) recombinant ROKa/ROCK-ll. The ...
Anti-phospho-CPI-17 (Thr38)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, Po, R, Rb | KA | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q96A00 # Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
SIZE: 147 amino acids; 16693 Da
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
SIMILARITY: SwissProt: Q96A0 ... see more »
SIZE: 147 amino acids; 16693 Da
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
SIMILARITY: SwissProt: Q96A0 ... see more »
Entrez Gene Number:
Entrez Gene Summary
PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM]
Description:
Anti-phospho-CPI-17 (Thr38)
Specificity:
CPI-17 phosphorylated on Threonine 38.
Key Applications:
Kinase Assay
Species Reactivity:
- Bovine
- Human
- Mouse
- Pig
- Rat
- Rabbit
Isotype:
IgG
Immunogen:
Peptide corresponding to amino acids 35-46 of human CPI-17.
Modifications:
Phosphorylation
Molecular Weight:
~17 kDa
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%.
Storage Conditions:
2 years at -20°C
Gene Symbol:
- PPP1R14A
- 17-KDasprotein
- CPI-17
- CPI17
- PPP1INL
Quality Assurance:
Routinely evaluated by immunoblot on phosphorylated recombinant CPI-17.
Antibody Category:
Cell Signaling
Antibody Sub-Category:
Cytoskeletal Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
The myosin phosphatase inhibitor protein, CPI-17, is expressed in smooth muscle, platelets and brain, and its phosphorylation at Thr38 enhances inhibitory potency 1000-fold. CPI-17 is an established substrate for PKC, ROCK, PKN, and ZIP-like kinase. It plays a central role in control of myosin phosphatase. Platelet activation and smooth muscle contraction in response to histamine both trigger phosphorylation of Thr38. Addition of CPI-17 to skinned smooth muscle restores PKC-stimulated contraction and expression of CPI-17 in fibroblasts is thought to cause stress fiber bundling.
Trade Name:
Upstate (Millipore)
Purification Method:
protein A purified
Format:
Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit