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WB: ROKa/ROCK-II only (Lane 1), MYPT1 substrate only (Lane 2), and MYPT1 substrate phosphorylated by ROKa/ROCK-II (Lane 3) were probed with anti-phosp ...
Anti-phospho-MYPT1 (Thr850)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M, R | WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.
SIZE: 1030 amino acids; 115281 Da
SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
PTM: Phosphorylated ... see more »
SIZE: 1030 amino acids; 115281 Da
SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
PTM: Phosphorylated ... see more »
Entrez Gene Number:
Entrez Gene Summary
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-
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Description:
Anti-phospho-MYPT1 (Thr850)
Specificity:
Recombinant truncated chicken MYPT1 phosphorylated on Thr850.
Key Applications:
Western Blotting
Species Reactivity:
- Chicken
- Human
- Mouse
- Rat
Isotype:
IgG
Immunogen:
Peptide corresponding to amino acid residues 845-855 of chicken MYPT1; this sequence is identical to amino acids 848-858 of human MYPT1, with the phosphorylated Thr at position 853.
Modifications:
Phosphorylation
Molecular Weight:
80 kDa
Control:
MYPT1 substrate phosphorylated by ROKa/ROCK-II
Presentation:
Protein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- PPP1R12A
- MBS
- MGC133042
- MYPT1
Quality Assurance:
Routinely evaluated by immunoblot on phophorylated MYPT1 substrate
Antibody Category:
Cell Signaling
Antibody Sub-Category:
Kinases & Phosphatases
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
0.87 mg/mL
Format Code:
Pur
Trade Name:
Upstate (Millipore)
Purification Method:
Protein A Purified
Format:
Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit