Ordering Information
Product Images
IB: Untreated (Lane 1) and anisomycin treated (Lane 2) 3T3 cell lysates were probed with anti-phospho-c-Jun (1:500 dilution).
Anti-phospho-c-Jun (Thr91)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Sheep | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P05412 # Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
SIZE: 331 amino acids; 35676 Da
SUBUNIT: Interacts with FOS to form a dimer. Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA.
SUBCELLULAR LOCATI ... see more »
SIZE: 331 amino acids; 35676 Da
SUBUNIT: Interacts with FOS to form a dimer. Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA.
SUBCELLULAR LOCATI ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Description:
Anti-phospho-c-Jun (Thr91)
Specificity:
phospho-c-Jun
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Predicted to cross-react with rat and bovine based on sequence homology
Isotype:
IgG
Immunogen:
peptide corresponding to amino acids 85-101 (NGHITT[pT]PTPTQFLCPK) of human c-jun
Modifications:
Phosphorylation
Molecular Weight:
41kDa
Presentation:
0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
1 year at -20°C
Gene Symbol:
- JUN
- p39
- AP1
- c-Jun
Quality Assurance:
routinely evaluated by immunoblot in RIPA lysates from 50mM cells that had been treated with anisomycin
Antibody Category:
Nuclear Function
Antibody Sub-Category:
Transcription Factors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Jun is a component of the transcription factor AP-1, and can constitute AP-1 activity either as a homodimer or as a heterodimer with Fos. By contrast with Fos, Jun is expressed constitutively, and can be activated by phosphorylation of serines 63 and/or 73. These sites are targets for the SAPK1/JNK family of protein kinases, and their phosphorylation states can be monitored using antibodies specific for the phospho-epitopes. In addition, Jun is phosphorylated by GSK3 on sites which inhibit Jun activity. Since GSK3 is inactivated by Akt, the Akt pathway results in Jun activation.
Trade Name:
Upstate (Millipore)
Purification Method:
immunoaffinity purified
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Sheep