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Catalogue Number : 07-007
Qty/Pk : 200 µg
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WB: Lambda phosphatase treated (Lane 1), and untreated (Lane 2), HeLa cell nuclear extract was probed with anti-phospho-BRCA1 (Ser 1497) at 1µg/ml

Anti-phospho-BRCA1 (Ser1497)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rabbit	Purified	Polyclonal Antibody

UniProt Number:

P38398

UniProt Summary

FUNCTION: SwissProt: P38398 # Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Mediates E2-dependent ubiquitination. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
SIZE: 1863 amino acids; 207721 Da
SUBUNIT: Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. CTIP interacts specifically with the BRCT domains. Interacts with RNA polymerase II holoenzyme. Interacts with SMC1A and COBRA1/NELFB. Binds BRIP1 through the BRCT domains. Interacts with ubiquitinated FANCD2. Interacts with BAP1. Interacts with DCLRE1C/Artemis and CLSPN. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with CHEK1/CHK1. Interacts with BRCC3. Interacts through its BRCT domains with phosphorylated ACACA and prevents its dephosphorylation.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: P38398 The RING-type zinc finger domain interacts with BAP1.
PTM: Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
DISEASE: "SwissProt: P38398 # Defects in BRCA1 are a cause of genetic susceptibility to breast cancer (BC) [MIM:113705, 114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination. & Defects in BRCA1 are a cause of genetic susceptibility to breast-ovarian cancer (BOC) [MIM:113705]. Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast- ovarian cancer. & Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705]."
SIMILARITY: Contains 2 BRCT domains. & Contains 1 RING-type zinc finger. ... see more »

Entrez Gene Number:

NM_007294

Entrez Gene Summary

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a ... see more »

Description:

Anti-phospho-BRCA1 (Ser1497)

Specificity:

phosphorylated BRCA1

Key Applications:

Western Blotting

Species Reactivity:

Human

Isotype:

IgG

Immunogen:

KLH-conjugated, synthetic phospho-peptide corresponding to residues 1489-1500 of human BRCA1 (C-KEPGVERS[pS]PSK)

Modifications:

Phosphorylation

Molecular Weight:

250kDa

Presentation:

of 0.1M Tris glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of 30% glycerol

Storage Conditions:

2 years at -20°C

Gene Symbol:

BRCA1
RNF53
BRCAI
PSCP
BRCC1
IRIS

Quality Assurance:

routinely evaluated by immunoblot on HeLa Nuclear Extract

Antibody Category:

Nuclear Function

Antibody Sub-Category:

Cell Cycle, DNA Replication & Repair

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Descriptive Text:

Approximately 50% of breast and ovarian cancers can be accounted for by mutations in the breast and ovarian cancer susceptibility genes BRCA1 and BRCA2. BRCA1 encodes a nuclear phosphoprotein which functions as a tumor suppressor. Transfection of BRCA1 into transformed cells has been reported to flatten morphology and reduce anchorage-independent growth, while expression of an anti-sense construct in normal fibroblasts achieves the reverse. BRCA1 works closely with Rad51, which plays an important role in repairing genetic damage in cells, suggesting a role for BRCA1 in the control of DNA recombination and of genome integrity.

Trade Name:

Upstate (Millipore)

Purification Method:

protein A chromatography

Format:

Purified

Antibody Type:

Polyclonal Antibody

Host:

Rabbit