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WB: untreated (lane 1) and anisomycin-treated (lane 2) 3T3 cell lysate was probed with anti-phospho-Jun (Ser73) at 1:1000 dilution.
Anti-phospho-c-Jun (Ser73)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB, IC | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P05412 # Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
SIZE: 331 amino acids; 35676 Da
SUBUNIT: Interacts with FOS to form a dimer. Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA.
SUBCELLULAR LOCATI ... see more »
SIZE: 331 amino acids; 35676 Da
SUBUNIT: Interacts with FOS to form a dimer. Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA.
SUBCELLULAR LOCATI ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Description:
Anti-phospho-c-Jun (Ser73)
Background Information:
c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced
signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
Specificity:
Recognizes phospho-c-Jun and phospho-JunD.
Key Applications:
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
Species Reactivity:
- Human
- Mouse
Isotype:
IgG
Immunogen:
BSA conjugated synthetic peptide (KLApSPELERLIIQC corresponding to amino acids 70-82 (pS73) of human c-Jun
Modifications:
Phosphorylation
Molecular Weight:
35-39 kDa
Control:
Anisomycin or UV-treated NIH-3T3 cells
Presentation:
Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.2, 0.15M NaCl, 0.05% sodium azide with 5mg/ml BSA
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- JUN
- p39
- AP1
- c-Jun
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates of 3T3/NIH cells serum starved overnight and treated anisomycin
Antibody Category:
Nuclear Function
Antibody Sub-Category:
Transcription Factors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Jun is a component of the transcription factor AP-1, and can constitute AP-1 activity either as a homodimer or as a heterodimer with Fos. By contrast with Fos, Jun is expressed constitutively, and can be activated by phosphorylation of serines 63 and/or 73. These sites are targets for the SAPK1/JNK family of protein kinases, and their phosphorylation states can be monitored using antibodies specific for the phospho-epitopes. In addition, Jun is phosphorylated by GSK3 on sites which inhibit Jun activity. Since GSK3 is inactivated by Akt, the Akt pathway results in Jun activation.
Concentration:
Variable
Format Code:
APur
Trade Name:
Upstate (Millipore)
Purification Method:
Immunoaffinity Purified
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit