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WB: Serum-stimulated (lane 1) and unstimulated (lane 2) NIH3T3 cell lysates were probed with Anti-phospho MAP Kinase/Erk1/2 (Tyr180), polyclonal at 2 ...
Anti-phospho-MAP Kinase1/2 (Erk1/2) (Tyr180), polyclonal
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IP, WB | Sheep | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 36 ... see more »
COFACTOR: Magnesium (By similarity).
SIZE: 36 ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated
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Description:
Anti-phospho-MAP Kinase1/2 (Erk1/2) (Tyr180), polyclonal
Specificity:
phosphorylated Erk1 and Erk2
Key Applications:
- Immunoprecipitation
- Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
Isotype:
IgG
Immunogen:
Synthetic peptide (CTGFLTEpYVAT) corresponding to residues 174-183 of human MAP Kinase
Modifications:
Phosphorylation
Molecular Weight:
Erk1 (42kDa) and Erk2 (44kDa)
Presentation:
0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
1 year at -20°C
Gene Symbol:
- MAPK1
- p38
- ERK
- P42MAPK
- ERK2
- p40
- PRKM1
- p41mapk
- PRKM2
- MAPK2
- p42-MAPK
- p41
- ERK-2
- ERT1
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates of NGF-stimulated PC-12 cells, mouse 3T3/A31 cells or human A431 cells
Antibody Category:
Cell Signaling
Antibody Sub-Category:
MAP Kinases
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Trade Name:
Upstate (Millipore)
Purification Method:
immunoaffinity chromatography
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Sheep