p-c-Jun (KM-1) Antibody sc-822

santa cruz biotechnology, inc.

Welcome! 0 Items in Cart

Advanced Search

home » Transcription Regulators » c-Jun Antibody / c-Jun Antibodies » p-c-Jun (KM-1): sc-822

p-c-Jun (KM-1): sc-822

Search | Datasheet

mouse monoclonal IgG₁; 200 µg/ml
epitope corresponding to amino acids 56-69 of human c-Jun
recommended for detection of c-Jun p39 phosphorylated on Serine 63 of m, r and h origin by WB, IP, IF, IHC(P) and FCM; non cross-reactive with Jun B or Jun D phosphorylated on the analogous serine residues or with c-Jun non-phosphorylated at Serine 63
TransCruz reagent for Gel Supershift and ChIP applications, sc-822 X, 200 µg/0.1 ml
HRP conjugate, sc-822 HRP, 200 µg/ml
fluorescein (sc-822 FITC) and phycoerythrin (sc-822 PE) conjugates for intracellular FCM, 100 tests
for phosphorylation positive (sc-2247) and non-activated control (sc-2210) cell extracts
Alexa Fluor® 405 (sc-822 AF405), Alexa Fluor® 488 (sc-822 AF488) and Alexa Fluor® 647 (sc-822 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml

Additional c-Jun Antibodies ...

Ordering Information Product Citations

Recommended Support Products:

(click button of application of choice)

WB IP IF IHC(P) FCM Gel Shift ChIP siRNA

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Human	JUN	3725	1p32.1	NM_002228	P05412	165160
Mouse	Jun	16476	4 C5		P05627	N/A

Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Wish List
p-c-Jun (KM-1)	sc-822	200 µg/ml	$259
p-c-Jun (KM-1) Alexa Fluor® 405	sc-822 AF405	100 µg/2 ml	$299
p-c-Jun (KM-1) Alexa Fluor® 488	sc-822 AF488	100 µg/2 ml	$299
p-c-Jun (KM-1) Alexa Fluor® 647	sc-822 AF647	100 µg/2 ml	$299
p-c-Jun (KM-1) FITC	sc-822 FITC	100 tests in 2 ml	$278
p-c-Jun (KM-1) HRP	sc-822 HRP	200 µg/ml	$259
p-c-Jun (KM-1) PE	sc-822 PE	100 tests in 2ml	$289
p-c-Jun (KM-1) X	sc-822 X	200 µg/0.1 ml	$259

siRNA Gene Silencers (click catalog # for product information)

Product Name	Catalog #	Unit	Price	Qty	Wish List
c-Jun siRNA (h)	sc-29223	10 µM	$245
c-Jun siRNA (m)	sc-29224	10 µM	$245
c-Jun (h)-PR	sc-29223-PR	10 µM	$22
c-Jun (m)-PR	sc-29224-PR	10 µM	$22

shRNA Plasmids (click catalog # for product information)

Product Name	Catalog #	Unit	Price	Qty	Wish List
c-Jun shRNA Plasmid (h)	sc-29223-SH	20 µg	$495
c-Jun shRNA Plasmid (m)	sc-29224-SH	20 µg	$495

shRNA Lentiviral Particles (click catalog # for product information)

Product Name	Catalog #	Unit	Price	Qty	Wish List
c-Jun shRNA (h) Lentiviral Particles	sc-29223-V	200 µl	$595
c-Jun shRNA (m) Lentiviral Particles	sc-29224-V	200 µl	$595

WB Positive Control Cell Lysate (s) (click catalog # for product information)

Product Name	Catalog #	Unit	Price	Qty	Wish List
A-431 + PMA nuclear extract	sc-2123	1000 µg	$136
NIH/3T3 Whole Cell Lysate	sc-2210	500 µg/200 µl	$99

p-c-Jun (KM-1) Product Citations

See how others have used p-c-Jun (KM-1): sc-822 antibody and or p-c-Jun (KM-1) antibody conjugates.

displaying 1 - 5 of 123 results : < next 5 citations >

Andreolas, C. et al. 2008. Fra-1 regulates vimentin during Ha-RAS-induced epithelial mesenchymal transition in human colon carcinoma cells. Int J Cancer. 122(8): 1745-1756. View PubMed Entry

Lindeman, J.H. et al. 2008. Enhanced expression and activation of pro-inflammatory transcription factors distinguish aneurysmal from atherosclerotic aorta: IL-6- and IL-8-dominated inflammatory responses prevail in the human aneurysm. Clin Sci (Lond). 114: 687-697. View PubMed Entry

Chen L et al. 2008 . Cadmium activates the mitogen-activated protein kinase (MAPK) pathway via induction of reactive oxygen species and inhibition of protein phosphatases 2A and 5. Free Radic Biol Med. . 45: 1035-1044. View PubMed Entry

Xia, Y. et al. 2007. MEKK1 mediates the ubiquitination and degradation of c-Jun in response to osmotic stress. Mol Cell Biol. . 27: 510-517. View PubMed Entry

Xia, Y. et al. 2007. MEKK1 Mediates the Ubiquitination and Degradation of c-Jun in Response to Osmotic Stress. Mol. Cell. Biol. 27(2): 510-517. View PubMed Entry

p-c-Jun (KM-1) Data

Click on image to enlarge

c-Jun (KM-1): sc-822. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing punctate nuclear staining of phosphorylated c-Jun (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma showing nuclear localization of activated c-Jun (B).

Western blot analysis of c-Jun activation in whole cell lysates from control (A) and anisomycin-treated (B) NIH/3T3 cells and phorbol ester-induced A-431 nuclear extracts (C,D,E). Antibodies tested include p-c-Jun (KM-1): sc-822 (A,B,C) and p-c-Jun (KM-1): sc-822 blocked with its cognate (D) or an unrelated peptide (E).

Western blot analysis of human recombinant c-Jun (A,C) and human recombinant c-Jun phosphorylated by human recombinant JNK1 (B,D) fusion proteins. Antibodies tested include c-Jun (N): sc-45 (A,B) and p-c-Jun (KM-1): sc-822 (C,D).

Intracellular FCM analysis of anisomyin-induced c-Jun Serine 63 phosphorylation: Pink dotted lines represent rabbit IgG PE (A) and mouse IgG₁ PE (B) stained anisomycin-treated NIH/3T3 cells. Green line histograms represent untreated NIH/3T3 cells (A,B). Solid orange histograms represent anisomycin-treated NIH/3T3 cells (A,B). Antibodies tested include: c-Jun (N) PE: sc-45 PE and p-c-Jun (KM-1): sc-822 PE.

Western blot analysis of human recombinant c-Jun (A,C) and human recombinant c-Jun phosphorylated by human recombinant JNK1 (B,D). Antibodies tested include c-Jun (N): sc-45 (A,B) and p-c-Jun (KM-1): sc-822 (C,D).

p-c-Jun (KM-1) Alexa Fluor 488: sc-822 AF488. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG₁: sc-3890.

p-c-Jun (KM-1): sc-822. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates.

Download

Datasheets

Get Adobe Acrobat Reader