Western blot analysis of phosphorylated ERK 1/2 expression in untreated Jurkat (A) and PMA-treated Jurkat (B,C) whole cell lysates. Blots were probed with p-ERK 1/2 (Thr 202/ Tyr 204):
sc-16982 (A,B) and p-ERK 1/2 (Thr 202/ Tyr 204):
sc-16982 preincubated with its cognate phosphorylated peptide (C).