Human Embryonic Stem Cell Research: Recent Progress and Future Directions of NIGMS Grantees
April 17-19, 2005
Workshop Summary
The purpose of this workshop was to provide an opportunity for NIGMS grantees conducting human embryonic stem cell (HESC) research to report on their recent progress, to exchange information, and to identify problems, challenges, and opportunities associated with this emerging area of research. The workshop brought together an unusually wide spectrum of scientists working on HESC that reflects the breadth of basic research supported by NIGMS. There were 68 participants, including NIGMS grantees (R01 awardees, recipients of supplements to research grants, and P20 Exploratory Center grantees) and members of their laboratories. Outside speakers and NIH staff members also were present. The agenda and list of participants are below.
Agenda
Sunday, April 17
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7:00 p.m. |
Welcome (Marion Zatz and James Battey) |
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Keynote Addresses |
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7:15 p.m. |
Stem Cells and the Evolution of the Cell Cycle (Andrew Murray) |
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8:05 p.m. |
A Role for Chemistry in Stem Cell Biology (Peter Schultz) |
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9:00 p.m. |
Reception |
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Monday, April 18 |
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7:45 a.m. |
Continental Breakfast |
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8:00 a.m. |
Opening Remarks (Jeremy Berg) |
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Session I: Stem Cell Self Renewal (Chair, Tony Blau) |
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8:15 a.m. |
Overview: What are ES Cells? (James Thomson) |
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8:35 a.m. |
Tyrosine Kinase Receptors (Tony Blau) |
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8:55 a.m. |
Role of Wnt Signaling (Randy Moon) |
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9:15 a.m. |
Molecular Analysis of Stemness (Ali H. Brivanlou) |
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9:35 a.m. |
Questions and Discussion |
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10:00 a.m. |
Coffee Break |
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Session II: Stem Cell Characterization and Standardization (Chair, Dr. Sue O’Shea) |
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10:30 a.m. |
Overview (Meri Firpo) |
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11:00 a.m. |
Comparison of Cell Lines - The Stability and Controlled Differentiation of Human ES Cells (Ron McKay) |
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11:20 a.m. |
Comparison of Cell Lines - UW/FHCRC HESC Core (Carol Ware) |
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11:40 a.m. |
Recent Progress in Improved Culture of HESC (Mark Levenstein) |
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12:00 p.m. |
Questions and Discussion |
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12:30 p.m. |
Working Lunch and Poster Setup |
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Session III: Stem Differentiation (Chair, Randy Moon) |
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1:30 p.m. |
Overview of Differentiation (Randy Moon) |
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1:45 p.m. |
Immuno-transcriptional Analysis of the Early Stages of Human Embryonic Stem Cell Differentiation (Andrew Laslett) |
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2:05 p.m. |
Derivation of Cardiomyocytes for Cardiac Repair (Charles Murry) |
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2:25 p.m. |
Trophoblast Differentiation (Ren-He Xu) |
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2:45 p.m. |
Keratinocytes from Human ES Cells (Howard Green) |
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3:05 p.m. |
Retrotransposon Mobility in Human ES Cells (John Moran) |
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3:25 p.m. |
Questions and Discussion |
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4:00 p.m. |
Break/Poster Setup |
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4:30-6:00 p.m. |
Poster Session and Refreshments |
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7:00 p.m. |
Optional Dinner |
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Tuesday, April 19 |
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Session IV: Cool Technologies (Chair, Rick Young) |
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8:15 a.m. |
Global Gene Regulation: Technology and Insights (Rick Young) |
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8:45 a.m. |
Whole Genome Transcriptional Analyses using Massively Parallel Signature Sequencing (MPSS) (Blake Meyers) |
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9:05 a.m. |
Novel RNAi approaches (Natasha Caplen) |
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10:05 a.m. |
Questions and Discussion |
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10:30 a.m. |
Session V: Scientific and Technical Challenges and Opportunities (Chair, Marion Zatz) |
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11:15 a.m. |
Workshop Summary (James Thomson) |
Participants List
Rosemary Akhurst UCSF San Francisco, CA 94143-0875 Phone: 415-514-0215 Fax: 415-502-6779 E-mail: rakhurst@cc.ucsf.edu
Donna Albertson Professor UCSF Box 0808 San Francisco, CA 94549 Phone: 415-502-8463 Fax: 415-476-8218 E-mail: albertson@cc.ucsfs.edu
Marc Antonyak Research Associate Cornell University Other Communication Department of Molecular Medicine C3 153 VMC Phone: 607-253-3795 Fax: 607-253-3659 E-mail: marcant225@aol.com
James Battey Director NIDCD - NIH 31 Center Drive Bethesda, MD 20892 Phone: 415-502-8463 Fax: 415-476-8218 E-mail: albertson@cc.ucsfs.edu
Klaus Becker Postdoctoral Fellow University of Massachusetts Medical School 55 Lake Avenue North Worchester, MA 01655 Phone: 508-856-3496 Fax: 508-856-6800 E-mail: laus.becker@umassmed.edu
Travis Berggren Scientist WiCell Research Institute P.O. Box 7365 Madison, WI 53707 Phone:608-261-1090 E-mail: travis@wicell.org
C. Anthony Blau Associate Professor of Medicine/Hematology University of Washington 1959 N.E. Pacific Street Seattle, WA 98195 Phone: 206-685-6873 Fax: 206-543-3560 E-mail: tblau@u.washington.edu
Megan Bodnar Staff Research Associate UCSF 533 Parnassus, Box 0720 San Francisco, CA 94143 Phone: 415-476-4733 Fax: 415-476-6951 E-mail: mbodnar@itsa.ucsf.edu
Ali Brivanlou Professor HOL The Rockefeller University CCR, NCI 1230 York Avenue New York, NY 10021 Phone: 212-327-8655 Fax: 212-327-8685 E-mail: brvnlou@rockefeller.edu
Natasha Caplen Senior Scientist Gene Silencing Section, OSTP, OD Bldg 37, Rm 3128B, 37 Covet Drive Bethesda, MD 20892 Phone: 301-451-1844 Fax: 301-402-1031 E-mail: ncaplen@mail.nih.gov
Emily Carlson Science Writer NIGMS Room 3AN.32C, 45 Center Drive Bethesda, MD 20892-6200 Phone: 301-594-1515 Fax: 301-402-0224 E-mail: carlsone@nigms.nih.gov
Anthony Carter Program Director NIGMS 45 Center Drive, MSC 6200 Bethesda, MD 20892-6200 Phone: 301-594-0943 Fax: 301-480-2228 E-mail: cartera@nigms.nih.gov
Richard Cerione Professor Cornell University Department of Molecular Medicine C3 155 VMC Medical School Ithaca, NY 14850-6401 Phone: 607-253-3888 Fax: 607-253-3659 E-mail: rac1@cornell.edu
Andrew J. Chess Associate Professor of Medicine Whitehead Institute for Biomedical Research Center for Human Genetic Research Harvard University Cambridge, MA 02142 Phone: 617-258-7748 Fax: 617-258-6505 E-mail: achess@partners.org
Sheng Ding Assistant Professor The Scripps Research Institute 1055 N. Torrey Pines Road TSRI, SR202 La Jolla, CA 92032 Phone: 858-784-7376 Fax: 858-784-9440 E-mail: sding@scripps.edu
Irene Eckstrand Program Director NIGMS 45 Center Drive Bethesda, MD 20892 Phone: 301-594-0943 Fax: 301-480-2228 E-mail: eckstrai@mail.nih.gov
Xin-Hua Feng Associate Professor Baylor College of Medicine One Baylor Plaza Houston, TX 77030 Phone: 713-798-4756 Fax: 713-798-4093 E-mail: xfeng@bcm.tmc.edu
Gen-Sheng Feng Professor Burnham Institute 10901 N. Torrey Pines Road La Jolla, CA 92037 Phone: 858-713-6265 Fax: 858-713-6274 E-mail: gfeng@burnham.org
Meri Firpo Assistant Professor UCSF 533 Parnassus Street San Francisco, CA 94143 Phone: 415-476-4733 Fax: 415-476-6145 E-mail: firpo@itsa.ucsf.edu
David Gilbert Professor SUNY Upstate Medical University Department of Biochemistry 750 East Adams Street Syracuse, NY 13210 Phone: 315-464-8723 Fax: 315-464-8750 E-mail: gilbertd@upstate.edu
Robert Goldman Chairman, Department of Cell and Molecular Biology Northwestern University, Feinberg School of Medicine 303 E. Chicago Avenue Ward W11-145 Chicago, IL 60611 Phone: 312-503-4215 Fax: 312-503-0954 E-mail: b-jaron@norhwestern.edu
Lawrence Goldstein Professor/Investigator University of California, San Diego, HHMI 9500 Gilman Drive, LBR 414 La Jolla, CA 92093 Phone: 858-534-9702 Fax: 858-534-9701 E-mail: lgoldstein@ucsd.edu
Howard Green Professor of Cell Biology, George Higginson Harvard Medical Schoo 240 Longwood Avenue Boston, MA 02115 Phone: 617-432-0851 Fax: 617-432-0109 E-mail: hgreen@hms.harvard.edu
Judith Greenberg Director, Division of Genetics and Developmental Biology NIGMS Building 45, Room 2AS.25 45 Center Drive MCS 6200 Bethesda, MD 20892-6200 Phone: 301-594-0343 Fax: 301-402-2228 E-mail: greenbej@nigms.nih.gov
Gary Grotendorst Senior Scientist Lovelace Respiratory Research Institute 2425 Ridgecrest Drive, S.E. Albuquerque, NM 87108 Phone: 505-348-9631 Fax: 505-348-8567 E-mail: ggrotend@lrri.org
Lisa Hall Co-Investigator UMMS - Department of Cell Biology 55 Lake North, Room S7-144 Worchester, MA 01655 Phone: 508-856-5439 Fax: 508-856-5178 E-mail: lisa.hall@umassmed.edu
Els Henckaerts Postdoctoral Fellow (EH) Mount Sinai School of Medicine 1 Gustave Levy Place, Box 1496 New York, NY 10029 Phone: 212-659-8278 Fax: 212-849-2437 E-mail: michael.linden@mssm.edu
Peter Jackson Associate Professor Stanford University School of Medicine MC5324,300 Pasteur Drive Palo Alto, CA 94305 Phone: 650-498-6872 Fax: 650-725-6902 E-mail: pjackson@stanford.edu
Nobuaki Kikyo University of Minnesota, Twin Cities MMC 716, 420 Delaware Street, S.E. Minneapolis, MN 55455 Phone: 612-624-0498 Fax: 612-624-2436 E-mail: kikyo001@umn.edu
Aimee Kohn Hematology Fellow University of Washington 12742A 35th Avenue, N.E. Seattle, WA 98125 Phone: 206-364-0275 Fax: 206-616-4230 E-mail: adkohn@u.washington.edu
Paul Krebsbach Associate Professor University of Michigan 1011 North University Avenue Ann Arbor, MI 48109-1078 Phone: 734-936-2600 Fax: 734-763-3453 E-mail: paulk@umich.edu
Andrew Laslett Laboratory of Embryonic Stem Cell Biology Centre for Reproduction and Development Monash Institute of Medical Research 3rd Floor, Building 75 STRIP Wellington Road Victoria, CANADA 3800 Phone: 613-9271-113 Fax: 613-9271-1198 E-mail: andrew.laslett@stemcellcentre.edu.au
Jeanne Lawrence Professor University of Massachusetts Medical School, Worcester Department of Cell Biology, 55 Lake Avenue North, S7-137 Worchester, MA 01655 Phone: 508-856-6015 Fax: 508-856-5178 E-mail: jeanne.lawrence@umassmed.edu
Mark Levenstein Postdoc WiCell Research Institute, Inc. P.O. Box 7365 Madison, WI 53707-7365 Phone: 608-262-4568 Fax: 608-262-8474 E-mail: markl@wicell.org
Catherine Lewis Chief, Biophysics Branch, CBB NIGMS 45 Center Drive MSC6200 Bethesda, MD 20892 Phone: 301-594-0828 Fax: 301-480-2004 E-mail: lewisC@nigms.nih.gov
Howard Lieberman Professor of Radiation Oncology Columbia University Health Sciences of Physicians and Surgeons 630 W. 168th Street New York, NY 10032 Phone: 212-305-9241 Fax: 212-342-5505 E-mail: lieberman@cuccfa.ccc.columbia.edu
R. Michael Linden Associate Professor (RML) Mount Sinai School of Medicine of NYU 1 Gustave Levy Place, Box 1496 New York, NY 10029 Phone: 212-659-8278 Fax: 212-849-2437 E-mail: michael.linden@mssm.edu
Dan Liu Instructor Baylor College of Medicine One Baylor Plaza Houston, TX 77030 Phone: 713-798-3486 Fax: 713-796-9438 E-mail: danl@bcm.tmc.edu
Ronald McKay Chief, Laboratory of Molecular Biology NINDS - NIH 9000 Rockville Pike Building 35, Room 3A201 Bethesda, MD 20892 Phone: 301-496-6574 Fax: 301-402-4738 E-mail: mckayr@ninds.nih.gov
Melissa Mendez Graduate Student, Northwestern University Department of Cell and Molecular Biology Ward W11-145 Chicago, IL 60611 Phone: 312-503-4215 Fax: 312-503-0954 E-mail: b-jaron@northwestern.edu
Blake Meyers Assistant Professor University of Delaware 15 Innovation Way, Room 230 Newark, DE 19711 Phone: 302-831-3418 Fax: 302-831-4841 E-mail: meyers@dbi.udel.edu
Annalisa Modanesi Research Specialist State University of New York at Stony Brook Department of Pharmacology BST, T-8, Room 140 Stony Brook, NY 11790-8651 Phone: 631-444-3489 Fax: 631-444-3234 E-mail: modanesi@pharm.sunysb.edu
Randall Moon Associate Professor University of Michigan 7726 MSII 0622 Ann Arbor, MI 48109-0622 Phone: 734-763-2021 E-mail: rmort@umich.edu
John V. Moran Associate Professor of Human Genetics and Internal Medicine University of Michigan Medical School 1241 E. Catherine Street Anne Arbor, MI 48109 Phone: 734-615-0456 Fax: 617-496-1541 E-mail: amurray@mcb.harvard.edu
Richard Mortensen Associate Professor University of Michigan 7726 MSII 0622 Ann Arbor, MI 48109-0622 Phone: 734-763-2021 E-mail: rmort@umich.edu
Andrew Murray Professor of Molecular and Cellular Biology Harvard University 16 Divinity Avenue, BL3000 Cambridge, MA 02138 Phone: 617-496-1350 Fax: 617-496-1541 E-mail: amurray@mcb.harvard.edu
Charles Murry Professor of Pathology, Director Center for Cardiovascular Biology & Regenerative Medicine University of Washington 815 Mercer Street, Room 453 Seattle, WA 98109 Phone: 206-616-8684 Fax: 206-897-1540 E-mail: murry@u.washington.edu
Curtis Omiecinski Professor Penn State University 201 Life Sciences Building University Park, PA 16802 Phone: 814-863-1625 Fax: 814-863-7696 E-mail: cjo10@psu.edu
Sue O’Shea Professor University of Michigan Cell and Development Biology Ann Arbor, MI 48109 Phone: 734-647-3990 Fax: 734-763-1166 E-mail: mdp141@psu.edu
Michael Packard Senior Research Technician Penn State University 201 Life Sciences Building University Park, PA 16802 Phone: 814-868-4234 Fax: 814-863-1357 E-mail: mdp141@psu.edu
Jeanine Page Postdoctoral Fellow Penn State University 201 Life Sciences Building University Park, PA 16802 Phone: 814-865-7121 Fax: 814-863-1357 E-mail: jle190@psu.edu
Jose Luis Garcia Perez Postdoctoral Researcher University of Michigan Medical School 1241 E. Catherine Street Ann Arbor, MI 48109 Phone: 734-615-0457 Fax: 734-763-3784 E-mail: jegp@umich.edu
David W. Russell Associate Professor University of Washington Seattle, WA 98195 Phone: 203-616-4562 Fax: 206-616-8298 E-mail: drussell@u.washington.edu
Kirstie Saltsman Science Writer NIGMS Building 45, Room 3AN.32 Bethesda, MD 20892-6200 Phone: 301-496-7301 Fax: 410-828-6472 E-mail: saltsmank@mail.nih.gov
Peter G. Schultz Los Angeles, CA 90048 E-mail: schultz@scripps.edu
M. Rocio Sierra-Honigman Director, Engineered Wound Repair Laboratory Cedars Sinai Medical Center Research Institute DAVIS 1091 8700 Beverly Boulevard Phone: 310-423-1882 Fax: 310-423-2325 E-mail: honigmannr@cshs.org
Janet Stein Professor University of Massachusetts Medical School 55 Lake Avenue North Worchester, MA 01655 Phone: 508-856-5625 Fax: 508-856-6800 E-mail: janet.stein@umassmed.edu
William M. Strauss Assistant Research Professor University of Colorado at Boulder 347 UCB Boulder, CA 80309 Phone: 303-735-5137 E-mail: william.m.strauss@colorado.edu
Michael Terns Associate Professor University of Georgia Anatomy Biochemistry and Molecular Biology 425 Athens, GA 30602 Phone: 706-542-1896 Fax: 706-542-1752 E-mail: mterns@bmb.uga.edu
James Thomson Professor University of Wisconsin-Madison 3420 Genetics-Biotechnology Center Medical School Henry Mall Madison, WI 53706 Phone: 608-263-3585 E-mail: thomson@primate.wisc.edu
Padmaja Vittal Graduate Fellow Penn State University 201 Life Sciences Building University Park, PA 16802 Phone: 814-863-4234 Fax: 814-863-1357 E-mail: padmaja@psu.edu
Hsien-yu Wang Research Associate Professor State University of New York at Stony Brook Deptartment of Physiology and Biophysics BST, T-5, Room 140 Stony Brook, NY 11790-8661 Phone: 631-444-3489 Fax: 631-444-3234 E-mail: wangh@pharm.sunysb.edu
Carol Ware Research Associate Professor University of Washington Comparative Medicine, Box 357190 Seattle, WA 98195 Phone: 202-616-5143 Fax: 202-685-3006 E-mail: cware@u.washington.edu
Ren-He Xu Senior Scientist WiCell Research Institute 8406 Castle Pines Drive Madison, WI 53717 Phone: 608-265-9312 Fax: 608-262-8474 E-mail: rxu@wicell.org
Richard Young Whitehead Insitute for Biomedical Research 9 Cambridge Center Cambridge, MA 02142 Phone: 617-258-5219 Fax: 617-258-0376 E-mail: young@wi.mit.eduZ
Junying Yu Assistant Scientist University of Wisconsin, Madison 425 Henry Hall, Room 4426 Madison, WI 53706 Phone: 608-890-0178 Fax: 608-890-0167 E-mail: jyu@primate.wisc.edu
Stephanie Zamule Graduate Fellow Penn State University 201 Life Sciences Building University Park, PA 16802 Phone: 814-863-4234 Fax: 814-863-1357 E-mail: smz136@psu.edu
Marion Zatz Chief, Developmental and Cellular Process Branch NIGMS 45 Center Drive MSC 6200 Bethesda, MD 20892-6200 Phone: 301-594-0943 Fax: 301-480-2228 E-mail: zatzm@nigms.nih.gov
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