Product Pathways - Ca / cAMP / Lipid Signaling
Phospho-PKD/PKCmu (Ser744/748) Antibody #2054
Applications | Reactivity | Sensitivity | MW (kDa) | Source |
---|---|---|---|---|
W | H M R Mk | Endogenous | 115 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
- 2054:
- Western Blotting
Specificity / Sensitivity
Phospho-PKD/PKC mu (Ser744/748) Antibody detects PKD1/PKC mu, PKD2, and PKD3/PKC eta only when dually phosphorylated at serines 744 and 748.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser744/748 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).
- Nishizuka, Y. (1984) Nature 308, 693-698.
- Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
- Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
- Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
- Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
- Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.
- Wood, C.D. et al. (2005) J Biol Chem 280, 6245-51.
Application References
- Zhukova, E. et al. (2001) Activation loop Ser744 and Ser748 in protein kinase D are transphosphorylated in vivo. J Biol Chem 276 (43), 40298-40305. This article references the use of Phospho-PKD/PKCmu (Ser744/748) Antibody in the following applications: Western Blotting
- Tan, M. et al. (2003) Thrombin rapidly induces protein kinase D phosphorylation, and protein kinase C delta mediates the activation. J. Biol. Chem. 278, 2824-2828. This article references the use of Phospho-PKD/PKCmu (Ser744/748) Antibody in the following applications: Western Blotting
- Waldron, R. T. et al. (2001) Protein kinase D potentiates DNA synthesis and cell proliferation induced by bombesin, vasopressin, or phorbol esters in Swiss 3T3 cells. J Biol Chem 276 (35), 32606-32615. This article references the use of Phospho-PKD/PKCmu (Ser744/748) Antibody in the following applications: Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.