Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PKD/PKCmu (Ser744/748) Antibody #2054

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 115 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Phospho-PKD/PKC mu (Ser744/748) Antibody detects PKD1/PKC mu, PKD2, and PKD3/PKC eta only when dually phosphorylated at serines 744 and 748.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser744/748 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml) or TPA-treated (0.2 µM), using Phospho-PKD/PKCµ (Ser744/748) Antibody (upper) or PKD/PKCµ Antibody #2052 (lower).

Background

Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).

  1. Nishizuka, Y. (1984) Nature 308, 693-698.
  2. Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
  3. Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
  4. Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
  5. Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
  6. Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.
  7. Wood, C.D. et al. (2005) J Biol Chem 280, 6245-51.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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