Clone ID: E228
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1228-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Rat
Mol. Weight: 68 kDa
UniProt ID: P49023
Alternative names:
Description: Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Tyr31 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 31.
Recommended Dilutions:
WB: 1:5000
IHC: 1:100-1:250
ICC: 1:250
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on NIH 3T3 cell lysate using anti-Phospho-Paxillin (pY31)RabMAb (cat. #1228-1); dilution 1:10,000. Cells were (1) untreated, and (2) treated with PDGF. |
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-Phospho-Paxillin (pY31) RabMAb (cat. #1228-1). |
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| C. Immunofluorescent staining of HeLa cells using anti-Phospho-Paxillin (pY31) RabMAb (cat. #1228-1). |
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Description References:
| 1. |
Salgia, R., et al. Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. J Biol Chem. 270: 5039 |
| 2. |
Burridge, K., et al. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119: 893 |
| 3. |
Bellis, S.L., et al. Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase. J. Biol. Chem. 270: 17437 |
| 4. |
Iwasaki, T., et al. Involvement of phosphorylation of Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration. Int J Cancer. 97: 330 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
