Clone ID: EP2113Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2156-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on Hela cell lysates using anti-MARCKS Phospho (pS159/163) RabMAb (cat. #2156-1), 1:2000 dilution. Cells were either (A) untreated (B) treated with TPA. |
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Recommended Applications: WB, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 87 kDa
UniProt ID: P29966
Alternative names: MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, PKCSL, 80K-L protein
Description: The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion; in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). As a filamentous (F) actin crosslinking protein, MARCKS binds to actin, calmodulin, and synapsin. In murine, MARCKS binding activity is regulated by PKC phosphorylation at Ser152, 156, and 163 of MARCKS during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion. After phospholyation, MARCKS is redistributed from plasma membrane to cytoplasm and is involved in leukocyte motility. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2). Specificity: A synthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein. The orthologous phosphorylation sites in mouse and rat is serine 152 and 156
Recommended Dilutions:
WB: 1:1000 - 2000
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Harlan DM, et al. J Biol Chem 266(22):14399-405, 1991 |
| 2. |
Hartwig JH, et al. Nature 356(6370):618-22, 1992. |
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877-772-2622 (US and Canada)
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orders@epitomics.com
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Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
