Product Pathways - Neuroscience
Phospho-Synapsin (Ser9) Antibody #2311
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M R (H) | Endogenous | 77 in brain mouse homogenate. | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 2311:
- Western Blotting
Specificity / Sensitivity
Phospho-Synapsin (Ser9) Antibody detects endogenous levels of synapsin only when phosphorylated at serine 9. The antibody is expected to recognize phosphorylated serine 9 of all synapsin isoforms.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding serine 9 of human synapsin I. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of mouse brain homogenates, untreated or phosphatase-treated, using Phospho-Synapsin (Ser9) Antibody (upper) or Synapsin Antibody #2312 (lower).
Background
Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of synapsin amino-terminal domain at serine 9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2312 Synapsin Antibody
- 2132 Parkin Antibody
- 2301 Phospho-DARPP-32 (Thr75) Antibody
- 2302 DARPP-32 Antibody
- 2304 Phospho-DARPP-32 (Thr34) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
