Experiment Detail Page

GENERAL EXPERIMENT INFORMATION

Title:	2-methoxyestradiol (2ME2) induces mammary gland differentiation through amphiregulin-EGFR mediated signaling
Identifier:	gov.nih.nci.ncicb.caarray:Experiment:1015897590892008:1
Experiment Date:	12/21/06
Experiment Design Type	time_series_design, development_or_differentiation_design, compound_treatment_design, dose_response_design,
Visibility	Public NCICB Dr. Jeff Green Lab
Description/Goal of the experiment:	2-methoxyestradiol (2ME2) induces mammary gland differentiation through amphiregulin-EGFR mediated signaling: molecular distinctions from the mammary gland of pregnant mice. High levels of 2ME2 are observed in the late stages of pregnancy. We investigated the role of 2ME2 on normal mammary gland development. Large scale gene expression assays were performed using Affymetrix GeneChips in pursuit of detailed molecular basis. (1) Mammary glands of wild type FVB mice administered 75 or 150 mg/kg of 2ME2 (2) Mammary glands of normal FVB/Nj mice (i) at day 16 of pregnancy, (ii) day 2 of lactation (iii) day 30 of post-lactation, and (3) mammary epithelial SCp2 cells after 6, 24 and 48 hours of 10 micromol 2ME2 treatment were examined. In vivo studies revealed that 2ME2 treatment up regulates the expression of amphiregulin. The clue to the role of 2ME2 in differentiation comes from studies in vitro which detected down regulation of inhibitor of differentiation (Id-1) gene and consequent up regulation of amphiregulin. The differentiation of E2 negative SCp2 cells by 2ME2 indicate estradiol independent mechanism. For details, please see our paper in Endocrinology 2006.

CONTACTS

Principal Investigator:	Jeffrey E Green
Contact Person:	Jung-Im Huh

HYBRIDIZATION FILE UPLOAD

HYBRIDIZATION FILES BATCH DOWNLOAD

CEL Files

Note: Do not download files immediately after uploading hybridization files. The application needs some time to process files.

ADDITIONAL DATA PROCESSING (DERIVED MEASUREMENTS)

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DataTransformation Protocol

Protocol Description

Uploaded files

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ADDITIONAL UPLOADED FILES

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File

Description

Action

1

prg2_4A_r2_upload.xls

2ME2 treatment in vivo: List of genes altered by 2-fold at p < 0.05. MS Excel file.

Download

2

prg2_4B_r2_upload.xls

In vitro 2ME2 treatment: List of genes significantly altered by 2-fold at p < 0.05.

Download

EXPERIMENTAL FACTORS/VARIABLES

Factor

Factor Type

Scale

Factor Value

2ME2_invivo

murine mammary gland

unscaled

Control

75 mg

150 mg

2ME2_invitro

cell_line

unscaled

Control6h

2ME2_6h

Control24h

2ME2_24h

Control48h

2ME2_48h

MMG_stages

murine mammary gland

unscaled

12W

P16

L2

ConW

W30

PUBLICATIONS

PubMed ID

Title

Authors

Publication

17158205

2-Methoxyestradiol Induces Mammary Gland Differentiation Through Amphiregulin-EGFR Mediated Signaling: molecular distinctions from the mammary gland of pregnant mice.

Huh JI, Qiu TH, Chandramouli GV, Charles R, Wiench M, Hager GL, Catena R, Calvo A, Lavallee TM, Desprez PY, Green JE.

Endocrinology

QUALITY CONTROL STEPS DESCRIPTION

Quality Control Description Type(s):

biological_replicate

Description:

Affymetrix recommondations were followed to accept the quality of arrays reported by GCOS or MAS5.

ADDITIONAL QUALIFIERS

Qualifier name:

Value:

Source

Experiment: 2-methoxyestradiol (2ME2) induces mammary gland differentiation through amphiregulin-EGFR mediated signaling