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WB: 20 micrograms of untreated (Lanes 1 & 3) and B-Raf phosphorylated (Lanes 2 & 4) PC-12 cell lysate was probed with a 1:5000 dilution of Anti-phosph ...
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
SIZE: 393 amino acids; 43439 Da
SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ ... see more »
SIZE: 393 amino acids; 43439 Da
SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal trans
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Description:
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226)
Specificity:
phosphorylated MEK1/MEK2
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
Isotype:
IgG
Immunogen:
Peptide containing DpSMANpSF in which pS corresponds to phosphorylated amino acids 218 and 222 of human MEK1
Modifications:
Phosphorylation
Molecular Weight:
45kDa
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
Gene Symbol:
- MAP2K1
- PRKMK1
- MKK1
- MAPKK1
- MEK1
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from PC-12 cells that have been treated with B-Raf
Antibody Category:
Cell Signaling
Antibody Sub-Category:
MAP Kinases
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Trade Name:
Upstate (Millipore)
Purification Method:
protein A purified
Format:
Purified
Antibody Type:
Monoclonal Antibody
Host:
Rabbit