Detection of Mouse MafB by IHC. Sample: FFPE section of mouse pancreatic islet. Antibody: Affinity purified rabbit anti-MafB (Cat. No. IHC-00351) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).
Detection of Mouse MafA by IHC. Sample: FFPE section of mouse pancreatic islet. Antibody: Affinity purified rabbit anti-MafA (Cat. No. IHC-00352) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).
Detection of Human Filamin B by IHC-IF. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Filamin B (Cat. No. IHC-00355) used at a dilution of 1:500. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 (A120-501E) used at a dilution of 1:100.
Immunohistochemistry Accessory Kit (Cat. No. IHC-101) provides the reagents and method for sensitive, reliable and economical immunohistochemical staining. This Kit is designed for immunostaining formalin-fixed, paraffin-embedded tissue sections with primary antibodies made in rabbit. It contains all the reagents required for the immunostaining procedure and will stain 250 slides.
Detection of Human and Mouse NRBF2 by Western Blot & Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: NRBF2 antibody A301-852A used for WB at 0.04 mcg/ml (A) & 1 mcg/ml (B) & used for IP at 3 mcg/mg lysate. NRBF2 was also immunoprecipitated by NRBF2 antibody A301-851A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds. (A&B).
Detection of Human and Mouse Cdc42GAP by Western Blot (h&m) and Immunoprecipitation (h).Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Cdc42GAP antibody A301-853A used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Cdc42GAP was also immunoprecipitated by Cdc42GAP antibody A301-854A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).
Detection of Human TRIM25 by Western Blot & Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: TRIM25 antibody A301-857A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. TRIM25 was also immunoprecipitated by TRIM25 antibodies A301-856A and A301-858A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) & 3 seconds (B).
ReliaBLOT® IP/Western Blot Reagents and Procedures (patent pending) provide an improved method for the detection of immunoprecipitated proteins assayed via Western Blot. It seamlessly integrates into existing IP/WB Protocols with three simple changes. It is optimized for antibodies made in rabbit.
