Clone ID: EP197Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2219-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on A431cell lysates using anti-Phospho-Erk1/p44 (pT202/204) RabMAb (cat. #2219-1), 1:2000 dilution. Cells were either (A) untreated (B) treated with EGF. |
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Recommended Applications: WB, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 42 kDa
UniProt ID: p27361
Alternative names: Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase
Description: Extra cellular signal-regulated kinases Erk1 (p44 MAPK) Serine/Threonine protein kinase is part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction (1,2,3). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) . Erk1 is also known to autophosphorylate on tyrosine (4). Specificity: A phospho specific peptide corresponding to residues surrounding Threonine 202 and 204 of human Erk1/p44. This antibody detects Erk1/p44 phosphorylated at Threonine 202 and 204.
Recommended Dilutions:
WB: 1:1000
IP: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Charest, D.L, et al. Mol Cell Biol. 13: 4679 |
| 2. |
Marshall, C.J. Cell 80: 179 |
| 3. |
Brott, B. K. et al. Cell Growth Differ. 4, 921-9294, 1993 |
| 4. |
Payne, D.M., et al. EMBO J. 10: 885 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
