Clone ID: E237
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1470-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 45 kDa
UniProt ID: Q02750
Alternative names: MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1
Description: MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Ser218 and Ser222 of human MEK1 was used as immunogen. The antibody only detects MEK1 phosphorylated on Serine 218 and 222.
Recommended Dilutions:
WB: 1:500 - 1000
IHC: 1:50-100
ICC: 1:50-100
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis A431 cell lysate using anti-MEK1 (pS218/222) RabMAb (cat. #1470-1); dilution 1:1000 (A) serum starved (negative control) (B) serum starved+ EGF |
B. Immunohistochemical analysis of paraffin-embedded skin carcinoma using anti-MEK1 RabMAb (cat. #1470-1). |
Product References:
| 1. | Mol. Cancer Ther., Jan 2007; 6: 138 - 146., Targeted inhibition of the extracellular signal-regulated kinase kinase pathway with AZD6244 (ARRY-142886) in the treatment of hepatocellular carcinoma ,Hung Huynh et. al. |
Description References:
| 1. |
Crews, C. M., et al. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258: 478 |
| 2. |
Zheng, C.F., and K.L. Guan. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J Biol Chem. 268: 11435 |
| 3. |
Zheng, C.F., and K.L. Guan. Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J. 13: 1123 |
| 4. |
Alessi, D.R., et al. Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. EMBO J. 13: 1610 |
| 5. |
Rosen, L.B., et al. Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras. Neuron 12: 1207 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
