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WB: 50ng of recombinant, unactive MAPKAP K2 (lane 1) and recombinant, active MAPKAP K2 (lane 2) probed with anti-phospho-MAPKAP Kinase 2 (Thr222) (0. ...
Anti-phospho-MAPKAP Kinase 2 (Thr222)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ht, M, Rb | KA, WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles
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Entrez Gene Number:
Entrez Gene Summary
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have b
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Description:
Anti-phospho-MAPKAP Kinase 2 (Thr222)
Background Information:
MAPKAP Kinase 2 has been implicted as a cell cycle checkpoint kinase, joining the ranks of Chk1 and Chk2 as critical regulators of the DNA damage response in mammalian cells in recent studies (Manke, et al, 2005, and Abraham, 2005). This research indicates that MAPKAP Kinase 2, a serine/threonine kinase activated by p38, phosphorylates CDC25 B/C, generating a binding site for 14-3-3 proteins. The checkpoint kinases Chk1 and Chk2 are targets for drug discovery efforts designed to overcome cell cycle arrest due to DNA damage induced by chemotherapeutic agents. Overriding the checkpoint kinases in cancer cells exposed to DNA damaging agents leads to catastrophic failure of cell division and apoptosis.
The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts.
The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts.
Specificity:
MAPKAP Kinase 2 phosphorylated at Thr222
Key Applications:
- Kinase Assay
- Western Blotting
Species Reactivity:
- Human
- Hamster
- Mouse
- Rabbit
Isotype:
IgG
Immunogen:
peptide (C-SHNSLT[pT]P) corresponding to amino acids 216-223 of phosphorylated human MAPKAP Kinase 2
Modifications:
Phosphorylation
Molecular Weight:
66kDa
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
Gene Symbol:
- MAPKAPK2
- MK2
- MAPKAPK-2
Quality Assurance:
routinely evaluated by immunoblot on recombinant MAPKAP Kinase 2, active (14-337), but did not detect recombinant MAPKAP Kinase 2, unactive (14-349)
Antibody Category:
Cell Signaling
Antibody Sub-Category:
MAP Kinases
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Trade Name:
Upstate (Millipore)
Purification Method:
Protein A chromatography
Format:
Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit