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Anti-phospho-STAT5A/B (Tyr694/699), clone 8-5-2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R, Sh | WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P51692 # Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
SIZE: 787 amino acids; 89866 Da
SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1, NMI and SOCS7.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocated into the nucle ... see more »
SIZE: 787 amino acids; 89866 Da
SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1, NMI and SOCS7.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocated into the nucle ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in d
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Description:
Anti-phospho-STAT5A/B (Tyr694/699), clone 8-5-2
Background Information:
The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation.
Clone:
8-5-2
Specificity:
Recognizes STAT5 phosphorylated on residue 694 of STAT5A and 699 of STAT5B.
Key Applications:
Western Blotting
Species Reactivity:
- Bovine
- Human
- Mouse
- Rat
- Sheep
Isotype:
IgG
Immunogen:
KLH-conjugated, synthetic phospho-peptide (KAVDG[pY]VKPQIK) corresponding to amino acids 689-700 of STAT5A and 694-705 of STAT5B
Modifications:
Phosphorylation
Molecular Weight:
STAT5A (94 kDa) and STAT5B (92 kDa)
Control:
IFN-α induced HeLa cells or GM-CSF treated TF-1 cells
Presentation:
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.5, 0.15M NaCl, 0.05% sodium azide
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- STAT5A
- MGF
- STAT5
Quality Assurance:
routinely evaluated by immunoblot on 3T3/A31 cells stimulated with PDGF or HeLa cells stimulated with interferon-α
Antibody Category:
Nuclear Function
Antibody Sub-Category:
Transcription Factors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Format Code:
Pur
Trade Name:
Upstate (Millipore)
Purification Method:
Protein G Purified
Format:
Purified
Antibody Type:
Monoclonal Antibody
Host:
Mouse