Product Pathways - Jak/Stat Pathway

Phospho-Stat5 (Tyr694) Antibody #9351

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• application references
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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W F	H M (R) (B)	Endogenous	90	Rabbit

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by Western blot.

Protocols

9351:

Flow, Western Blotting

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) Antibody detects endogenous levels of Stat5alpha and Stat5beta only when phosphorylated at Tyr694. The antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins, but does cross-react with EGFR.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr694 of mouse Stat5. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 has been found to be constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5α and β are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN-α in B cells, but only Stat5α is phosphorylated in response to IFN-α in HeLa cells (6).

1. Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369.
2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191.
3. Okutani, Y. et al. (2001) Oncogene 20, 6643-6650.
4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861.
5. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

Application References

• Fata, J. E. et al. (2000) The osteoclast differentiation factor osteoprotegerin-ligand is essential for mammary gland development. Cell 103, 41-50. This article references the use of Phospho-Stat5 (Tyr694) Antibody in the following applications: IHC-P (paraffin)
• Kapur, R. and Zhang, L. (2000) A novel mechanism of cooperation between c-Kit and erythropoietin receptor: Stem cell factor induces the expression of Stat5 and erythropoietin receptor resulting in efficient proliferation and survival by erythropoietin. J. Biol. Chem. 276, 1099-1106. This article references the use of Phospho-Stat5 (Tyr694) Antibody in the following applications: Western Blotting
• Siren, A. L. et al. (2001) Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress. Proc. Natl. Acad. Sci. USA 98, 4044-4049. This article references the use of Phospho-Stat5 (Tyr694) Antibody in the following applications: Western Blotting
• Reuther, G. W. et al. (2000) Identification and characterization of an activating TrkA deletion mutation in acute myeloid leukemia. Mol. Cell. Biol. 20, 8655-8666. This article references the use of Phospho-Stat5 (Tyr694) Antibody in the following applications: Western Blotting
• Bergonzini, V. et al. (2004) HIV-Tat promotes cellular proliferation and inhibits NGF-induced differentiation through mechanisms involving Id1 regulation. Oncogene 23, 7701-7711. This article references the use of Phospho-Stat5 (Tyr694) Antibody in the following applications: Western Blotting
• Brizzi, M. F. et al. (2002) Diabetic LDL inhibits cell-cycle progression via STAT5B and p21waf. J. Clin. Invest. 109 (1), 111. This article references the use of Phospho-Stat5 (Tyr694) Antibody in the following applications: IC-IF IHC-P (paraffin) Western Blotting
• Hutt, J. A. et al. (2000) Signal transducer and activator of transcription 3 activates CCAAT enhancer-binding protein delta gene transcription in G0 growth-arrested mouse mammary epithelial cells and in involuting mouse mammary gland. J. Biol. Chem. 275, 29123-29131. This article references the use of Phospho-Stat5 (Tyr694) Antibody in the following applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.