Product Pathways - Ca / cAMP / Lipid Signaling
Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody #2611
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 120 Phospho-PRK1. 140 Phospho-PRK2. | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 2611:
- Western Blotting
Specificity / Sensitivity
Phospho-PRK1 (Thr774)/ PRK2 (Thr816) Antibody detects endogenous levels of PRK1 and PRK2 only when phosphorylated at Thr774 or Thr816, respectively. This antibody also detects PKC zeta and lambda when phosphorylated at Thr 410 and 403, respectively.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr774 of human PRK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat cells, untreated or calf intestinal alkaline phosphotase (CIP)-treated, and 293 cells, untreated or calyculin A-treated, using Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody (upper) or PRK1/PRK2 antibody (lower).
Background
The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).
- Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
- Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
- Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
- Watanabe, G. et al. (1996) Science 271, 645-648.
- Amano, M. et al. (1996) Science 271, 648-650.
- Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
- Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
- Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
- Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
- Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.
Application References
- Yau, L. et al. (2003) Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression. Eur. J. Biochem. 270, 101-110. This article references the use of Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody in the following applications: Western Blotting
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Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2612 PRK2 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
