Product Pathways - MAPK Signaling
Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC F | H M R Sc Hm | Endogenous | 46 Phospho-JNK1. 54 Phospho-JNK2/3. | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Sc=S. cerevisiae
Hm=Hamster
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb detects endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185. This antibody does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase.
Source / Purification
Monoclonal antibody (isotype: mouse IgG1) is produced by immunizing mice with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK. The antibody is purified by affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells, untreated or UV-treated (lanes 1 and 2), NIH/3T3 cells, untreated or UV-treated (lanes 3 and 4) and C6 cells, untreated or anisomycin-treated (lanes 5 and 6), using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin-treated (blue), using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells untreated (left) and anisomycin-treated (right) using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (green). Actin filaments have been labeled with DY554 phalloidin (red).
Application References
- Akagi, T. et al. (2000) v-Crk activates the phosphoinositide 3-kinase/AKT pathway in transformation. Proc.Natl. Acad.Sci. USA 97, 7290-7295. This article references the use of Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb in the following applications: Western Blotting
- Song, J. S. et al. (1999) Tyrosine phosphorylation of Vav stimulates IL-6 production in mast cells by a Rac/c-Jun N-terminal kinase-dependent C208pathway. Journal of Immunology 163, 802-810. This article references the use of Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb in the following applications: Western Blotting
- Zhang, S. and Kaplan, M.H. (2000) The p38 mitogen-activated protein kinaseis required for IL-12-induced IFN-gamma expression. Journal of Immunology 165, 1374-1380. This article references the use of Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb in the following applications: Western Blotting
- Somasundaram, S. et al. (2002) Dietary Curcumin Inhibits Chemotherapy-induced Apoptosis in Models of Human Breast Cancer. Cancer Research 62, 3868-3875. This article references the use of Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb in the following applications: IHC-FL (floating/frozen)
- Xing, J. et al. (1998) Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation. Molecular and Cellular Biology 18, 1946-1955. This article references the use of Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb in the following applications: Western Blotting
- Saporito, M. S. et al. (2000) MPTP activates c-Jun NH(2)-terminal kinase (JNK) and its upstream regulatory kinase MKK4 in nigrostriatal neurons in vivo. J. Neurochem. 75, 1200-1208. This article references the use of Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4668 Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
- 9251 Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
- 9252 SAPK/JNK Antibody
- 9253 SAPK/JNK Control Cell Extracts
- 9258 SAPK/JNK (56G8) Rabbit mAb
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9250 PhosphoPlus® SAPK/JNK (Thr183/Tyr185) Antibody Kit
- 9912 Phospho-SAPK/JNK Pathway Sampler Kit
- 9810 SAPK/JNK Kinase Assay Kit (Nonradioactive)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
