Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-c-Abl (Thr735) Antibody #2864
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 135 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
- 2864:
- Western Blotting
Specificity / Sensitivity
Phospho-c-Abl (Thr735) Antibody detects endogenous levels of c-Abl or Bcr-Abl only when phosphorylated at threonine 735. The antibody does not cross-react with other related proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr735 of human c-Abl. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or TPA-treated (200 nM), using Phospho-c-Abl (Thr735) Antibody (upper) or c-Abl Antibody #2862 (lower).
Background
The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).
Thr735 is located within a conserved 14-3-3 protein binding motif region, and can be phosphorylated upon stress stimulation or TPA treatment. Phosphorylation at Thr735 may play an important role in regulating c-Abl localization as well as its function.
- Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
- Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
- Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
- Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
- Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
- Pluk, H. et al. (2002) Cell 108, 247-259.
Application References
- Raina, D. et al. (2006) EMBO J 25, 3774-83. This article references the use of Phospho-c-Abl (Thr735) Antibody in the following applications: Western Blotting
- Yoshida, K. et al. (2005) Nat Cell Biol 7, 278-85. This article references the use of Phospho-c-Abl (Thr735) Antibody in the following applications: Western Blotting
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Companion Products
- 2865 Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb
- 2868 Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb
- 3009 Phospho-c-Abl (Tyr204) (C42B5) Rabbit mAb
- 2861 Phospho-c-Abl (Tyr245) Antibody
- 2862 c-Abl Antibody
- 3098 Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
