BD Pharmingen

Antibodies and their Designated Sites (count: 89)
Gene/Protein Phosphorylation Sites Antibodies
Symbol
Name 		Site Name Alt Res Names Local Sequence MPR Site ID Refs Antibody Name (Cat#) Co. Polyclonal (species) OR Mono. (isotype; clone)
PARVA
Actopaxin
Genbank LocusLink 		Alt. gene names: parvin alpha; Actopaxin;
S8 SPQKpSPSVP 363
Actopaxin (pS8) (558374) Actopaxin (pS8) BD Pharmingen M (IgG1; J160-366)
AKT1
Akt1
Genbank LocusLink 		Alt. gene names: protein Kinase B; PKB; Akt1; AKT; C-AKT; RAC-alpha serine/threonine kinase; RAC; rac alpha; rac-pk alpha;
T308 ATMKpTFCGT 277 1, 4, 17
Akt (pT308) (558316) Akt (pT308) BD Pharmingen M (IgG1; J1-223.371)
Y326 EDNDpYGRAV 228
Akt (pY326) (558384) Akt (pY326) BD Pharmingen M (IgG2a; K7-642)
S473 FPQFpSYSAS 12 4, 14, 17, 18, 24, 27, 31, 32
Akt (pS472/pS473), Phospho-Specific (550747) Akt (pS472/pS473), Phospho-Specific BD Pharmingen M (IgG1; 104A282)
Akt (pS473) (558368) Akt (pS473) BD Pharmingen M (IgG1; J177-204.20)
SNCA
alpha synuclein
Genbank LocusLink 		Alt. gene names: alpha synuclein; non-A-beta component of Alzheimer disease amyloid precursor; NACP;
Y125 DNEApYEMPS 362
aSynuclein (PY125) (558246) aSynuclein (PY125) BD Pharmingen M (IgG2b; I57-628)
BCR
BCR
Genbank LocusLink 		Alt. gene names: breakpoint cluster region; BCR;
Y177 EKPFpYVNVE 364
BCR (pY177) (558248) BCR (pY177) BD Pharmingen M (IgG2b; J52-309)
ITGB3
beta-3 integrin
Genbank LocusLink 		Alt. gene names: CD61; platelet glycoprotein IIIa; GPIIb/IIIa; GP3A; antigen CD61; CD61A;
Y785 Y759 TNITpYRGT- 280
Integrin b3 (pY759), Phospho-Specific (612528) Integrin b3 (pY759), Phospho-Specific BD Pharmingen M (IgG1; 7a)
DAG1
beta-dystroglycan
Genbank LocusLink 		Alt. gene names: beta-dystroglycan; beta-DG; dystrophin-associated glycoprotein 1; DAG1; 156DAG; A2; alpha dystroglycan;
Y892 Y239 SPPPpYVPP- 314
beta-Dystroglycan (pY892), Phospho-Specific (612524) beta-Dystroglycan (pY892), Phospho-Specific BD Pharmingen M (IgG1; 27.1)
BLNK
blnk
Genbank LocusLink 		Alt. gene names: B cell linker protein; blnk; slp-65;
Y84 DSEMpYVMPA 365
BLNK (pY84) (558366) BLNK (pY84) BD Pharmingen M (IgG2b; J117-1278)
BTK
Btk
Genbank LocusLink 		Alt. gene names: Bruton's tyrosine kinase; Btk; B-cell progenitor kinase; bpk; agammaglobulinemia tyrosine kinase; atk;
Y551 LDDEpYTSSV 366
Btk (pY551) & Itk (pY511) (558034) Btk (pY551) & Itk (pY511) BD Pharmingen M (IgG1; 24a/BTK (Y551))
KIT
c-Kit
Genbank LocusLink 		Alt. gene names: CD117; stem cell factor receptor; SCFR;
Y568 NGNNpYVYID 370
CD117 (pY568/pY570) (c-Kit) (558390) CD117 (pY568/pY570) (c-Kit) BD Pharmingen M (IgG1; K39-686)
Y570 NNYVpYIDPT 371
CD117 (pY568/pY570) (c-Kit) (558390) CD117 (pY568/pY570) (c-Kit) BD Pharmingen M (IgG1; K39-686)
SRC
c-src
Genbank LocusLink 		Alt. gene names: cellular src; src; c-src; phosphoprotein 60 c-src; pp60c-src; pp60src; human homolog of v-src; Rous sarcoma virus; avian sarcoma virus; ASV;
Y530 Y529, Y527 TEPQpYQPGE 48 33
Fyn (pY528)/c-Src (pY530) (612669) Fyn (pY528)/c-Src (pY530) BD Pharmingen M (IgG2b; 31)
CAV1
caveolin-1
Genbank LocusLink 		Alt. gene names: caveolin-1; VIP21; caveolin;
Y14 EGHLpYTVPI 222
Caveolin (pY14), Phospho-Specific (611338) Caveolin (pY14), Phospho-Specific BD Pharmingen M (IgG1; 56)
CAV2
caveolin-2
Genbank LocusLink 		Alt. gene names: caveolin-2;
Y27 SGLEpYADPE 368
Caveolin 2 (pY27) (558364) Caveolin 2 (pY27) BD Pharmingen M (IgG1; 40/Caveolin 2)
CBL
cbl
Genbank LocusLink 		Alt. gene names: cbl; p120 cbl; c-Cbl;
Y700 EDTEpYMTPS 233
c-Cbl (pY700), Phospho-Specific (612304) c-Cbl (pY700), Phospho-Specific BD Pharmingen M (IgG1; 47)
Y774 EDDGpYDVPK 237
c-Cbl (pY774) (558035) c-Cbl (pY774) BD Pharmingen M (IgG1; 29/c-Cbl (Y774))
IL6ST
CD130
Genbank LocusLink 		Alt. gene names: CD130; IL-6R-gp130SIG; IL-6R beta; IL-6ST; gp130;
S782 SRSEpSTQPL 378
gp130 (pS782) (558096) gp130 (pS782) BD Pharmingen M (IgG2b; 6a/gp130 (pS782))
PTPRC
CD45
Genbank LocusLink 		Alt. gene names: CD45; B220; CD45R; CD45RA; CD45RB; CD45RC; CD45RO; EC 3.1.3.4; Leukocyte Common Antigen; LCA; T200; Ly5;
S999 SEHDpSDESS 369
CD45 (pS999) (Leukocyte Common Antigen, Ly-5) (558376) CD45 (pS999) (Leukocyte Common Antigen, Ly-5) BD Pharmingen M (IgG1; J143-1270)
CDC2
cdk1
Genbank LocusLink 		Alt. gene names: cyclin-dependent kinase 1; CDK1; cell division control protein 2 homolog; cdc2; p34 protein kinase;
Y15 GEGTpYGVVY 14 12, 15, 16, 19, 26
Cdc2 (pY15), Phospho-Specific (612306) Cdc2 (pY15), Phospho-Specific BD Pharmingen M (IgG1; 44)
CREB1
CREB-alpha
Genbank LocusLink 		Alt. gene names: cyclic AMP response element binding protein 1; CREB; CREB 1;
S133 SRRPpSYRKI 265 2, 13, 20, 21, 28, 30, 35
CREB (pS133) (558359) CREB (pS133) BD Pharmingen M (IgG1; J151-21)
CREB1
CREB-delta
Genbank LocusLink 		Alt. gene names: cyclic AMP response element binding protein 1; CREB; CREB 1;
S133 SRRPpSYRKI 265 2, 13, 20, 21, 28, 30, 35
CREB (pS133) (558359) CREB (pS133) BD Pharmingen M (IgG1; J151-21)
CRKL
CrkL
Genbank LocusLink 		Alt. gene names: CrkL; v-crk avian sarcoma virus CT10 oncogene homolog-like;
Y207 PAHApYAQPQ 225
CrkL (pY207) (558386) CrkL (pY207) BD Pharmingen M (IgG2a; K30-391.11.30)
CTNND1
delta 1 catenin
Genbank LocusLink 		Alt. gene names: delta 1 catenin; p120 catenin;
Y96 SHLLpYSTIP 316
p120 Catenin (pY96), Phospho-Specific (612534) p120 Catenin (pY96), Phospho-Specific BD Pharmingen M (IgG1; 25a)
Y228 GSDNpYGSLS 313
p120 Catenin (pY228), Phospho-Specific (612536) p120 Catenin (pY228), Phospho-Specific BD Pharmingen M (IgG1; 21a)
Y280 HPEPpYGLED 315
p120 Catenin (pY280), Phospho-Specific (612538) p120 Catenin (pY280), Phospho-Specific BD Pharmingen M (IgG1; 18)
MAPK3
erk1
Genbank LocusLink 		Alt. gene names: extracellular signal-regulated kinase 1; ERK1; mitogen activated protein kinase 3; MAPK3;
T202 T183, T185 TGFLpTEYVA 1
ERK1/2 (pT202/pY204), Phospho-Specific (612358) ERK1/2 (pT202/pY204), Phospho-Specific BD Pharmingen M (IgG1; 20A)
Y204 Y185, Y187 FLTEpYVATR 2
ERK1/2 (pT202/pY204), Phospho-Specific (612358) ERK1/2 (pT202/pY204), Phospho-Specific BD Pharmingen M (IgG1; 20A)
MAPK1
erk2
Genbank LocusLink 		Alt. gene names: extracellular signal-regulated kinase 2; ERK2; mitogen activated protein kinase 1; MAPK1;
T185 T183 TGFLpTEYVA 4 23
ERK1/2 (pT202/pY204), Phospho-Specific (612358) ERK1/2 (pT202/pY204), Phospho-Specific BD Pharmingen M (IgG1; 20A)
Y187 Y185 FLTEpYVATR 5
ERK1/2 (pT202/pY204), Phospho-Specific (612358) ERK1/2 (pT202/pY204), Phospho-Specific BD Pharmingen M (IgG1; 20A)
VIL2
ezrin
Genbank LocusLink 		Alt. gene names: Ezrin; VILLIN-2; cytovillin; pp81;
Y354 Y353 RLQDpYEEKT 361
Ezrin (pY353) (558033) Ezrin (pY353) BD Pharmingen M (IgG1; I66-386)
T567 DKYKpTLRQI 323
Ezrin (pT567) (558357) Ezrin (pT567) BD Pharmingen M (IgG1; J37-954.281.307)
FADD
FADD
Genbank LocusLink 		Alt. gene names: fas associating death domain containing protein; FADD;
S194 SGAMpSPMSW 290
FADD (pS194) (558370) FADD (pS194) BD Pharmingen M (IgG1; J119-857.36)
PTK2
FAK
Genbank LocusLink 		Alt. gene names: focal adhesion kinase; FAK; FADK; pp125fak; focal adhesion kinase 1; FAK1;
Y397 ETDDpYAEII 27 29
FAK (pY397), Phospho-Specific (611722) FAK (pY397), Phospho-Specific BD Pharmingen M (IgG1; 14)
FYN
fyn
Genbank LocusLink 		Alt. gene names: fyn; P59-fyn; slk; syn;
Y531 Y528, Y476 TEPQpYQPGE 377
Fyn (pY528)/c-Src (pY530) (612669) Fyn (pY528)/c-Src (pY530) BD Pharmingen M (IgG2b; 31)
GSK3A
GSK-3alpha
Genbank LocusLink 		Alt. gene names: glycogen synthase kinase-3 alpha; GSK-3alpha;
Y279 PNVSpYICSR 35
GSK-3b (pY216), Phospho-Specific (612312) GSK-3b (pY216), Phospho-Specific BD Pharmingen M (IgG1; 13a)
GSK3B
GSK-3beta
Genbank LocusLink 		Alt. gene names: glycogen synthase kinase-3 beta; GSK-3beta; Zw3;
Y216 Y279 PNVSpYICSR 312
GSK-3b (pY216), Phospho-Specific (612312) GSK-3b (pY216), Phospho-Specific BD Pharmingen M (IgG1; 13a)
NFKBIA
IkB alpha
Genbank LocusLink 		Alt. gene names: I kappa B alpha; IkBa; IkappaBalpha;
S32 DRHDpSGLDS 94
IkBa (pS32/pS36), Phospho-Specific (551818) IkBa (pS32/pS36), Phospho-Specific BD Pharmingen M (IgG1; 39A1431)
S36 SGLDpSMKDE 95
IkBa (pS32/pS36), Phospho-Specific (551818) IkBa (pS32/pS36), Phospho-Specific BD Pharmingen M (IgG1; 39A1431)
IRS1
IRS-1
Genbank LocusLink 		Alt. gene names: insulin-receptor signaling 1; insulin receptor substrate 1; IRS-1;
Y896 SPGEpYVNIE 108
IRS-1 (pY896) (558378) IRS-1 (pY896) BD Pharmingen M (IgG2a; K9-211)
ITK
itk
Genbank LocusLink 		Alt. gene names: IL-2 inducible T-cell kinase; itk; T-cell specific kinase; tsk; emt; lyk;
Y512 LDDQpYTSST 367
Btk (pY551) & Itk (pY511) (558034) Btk (pY551) & Itk (pY511) BD Pharmingen M (IgG1; 24a/BTK (Y551))
MAPK8
JNK1
Genbank LocusLink 		Alt. gene names: jun N-terminal kinase1; JNK1; c-Jun N-terminal kinase 1; mitogen activated protein kinase 8; MAPK8; protein kinase, mitogen-activated 8; PRKM8; stress activated protein kinase JNK1; SAPK1;
T183 SFMMpTPYVV 6
JNK (pT183/pY185), Phospho-Specific (612540) JNK (pT183/pY185), Phospho-Specific BD Pharmingen M (IgG1; 41)
Y185 MMTPpYVVTR 7
JNK (pT183/pY185), Phospho-Specific (612540) JNK (pT183/pY185), Phospho-Specific BD Pharmingen M (IgG1; 41)
JUN
jun
Genbank LocusLink 		Alt. gene names: jun; c-jun; v-jun; AP-1;
S63 DLLTpSPDVG 137
c-Jun (pS63) (558036) c-Jun (pS63) BD Pharmingen M (IgG1; 2)
LAT
LAT
Genbank LocusLink 		Alt. gene names: linker for activation of T cells; LAT; p36/38; p38; pp36/38;
Y200 Y170, Y171 SIDDpYVNVP 224 22
LAT (pY171) (558392) LAT (pY171) BD Pharmingen M (IgG1; I58-1169)
Y255 Y226, Y225 GAPDpYENLQ 379
LAT (pY226) (558363) LAT (pY226) BD Pharmingen M (IgG1; J96-1238.58.93)
LCK
lck
Genbank LocusLink 		Alt. gene names: Lck; p56lck; lymphocyte specific protein tyrosine kinase;
Y505 Y504 TEGQpYQPQP 231
Lck (pY505), Phospho-Specific (612390) Lck (pY505), Phospho-Specific BD Pharmingen M (IgG1; 4)
MARCKS
MARCKS
Genbank LocusLink 		Alt. gene names: myristoylated alanine-rich protein kinase C substrate; MARCKS; PKCSL;
S159 S152 KKRFpSFKKS 299
MARCKS (pS152/pS156) (558380) MARCKS (pS152/pS156) BD Pharmingen M (IgG1; I84-1233)
S163 S156 SFKKpSFKLS 300
MARCKS (pS152/pS156) (558380) MARCKS (pS152/pS156) BD Pharmingen M (IgG1; I84-1233)
MAP2K1
MEK1
Genbank LocusLink 		Alt. gene names: MEK1; MKK1;
S298 GRPLpSSYGM 380
MEK1 (pS298) (558375) MEK1 (pS298) BD Pharmingen M (IgG1; J114-64)
MSN
moesin
Genbank LocusLink 		Alt. gene names: MEMBRANE-ORGANIZING EXTENSION SPIKE PROTEIN; moesin; pasin 2;
T558 DKYKpTLRQI 184
Ezrin (pT567) (558357) Ezrin (pT567) BD Pharmingen M (IgG1; J37-954.281.307)
TBK1
NAK
Genbank LocusLink 		Alt. gene names: NF-kB-activating kinase; NAK; TANK-binding kinase 1; TBK1; T2K;
S172 EQFVpSLYGT 389
TBK1 (pS172) (558397) TBK1 (pS172) BD Pharmingen M (IgG2b; J133-1171)
NOS3
NOS3
Genbank LocusLink 		Alt. gene names: nitric oxide synthetase 3; NOS3; endothelial NOS; eNOS;
T495 TRKKpTFKEV 317
eNOS (pT495), Phospho-Specific (612706) eNOS (pT495), Phospho-Specific BD Pharmingen M (IgG1; 31)
S1177 IRTQpSFSLQ 67 7
eNOS (pS1177), Phospho-Specific (612392) eNOS (pS1177), Phospho-Specific BD Pharmingen M (IgG1; 19)
RASA1
p120 rasGAP
Genbank LocusLink 		Alt. gene names: p120 rasGAP; p120rasGAP; p120 GAP; p120GAP;
Y460 GKEIpYNTIR 383
Ras-GAP (pY460) (612737) Ras-GAP (pY460) BD Pharmingen M (IgG1; 19A)
BCAR1
p130CAS
Genbank LocusLink 		Alt. gene names: Crk-associated substrate p130Cas; p130CAS; CAS;
Y249 PQDIpYDVPP 393
p130Cas (pY249) (558401) p130Cas (pY249) BD Pharmingen M (IgG2b; J169-757.12.2)
MAPK14
p38-alpha
Genbank LocusLink 		Alt. gene names: p38 MAPK; p38-alpha; CSAID binding protein1; CSBP1; mitogen-activated protein kinase 14; MAPK14; MAX-interacting protein 2; MXI2; SAPK2A;
T180 T182 DDEMpTGYVA 10 11, 34
p38 MAPK (pT180/pY182), Phospho-Specific (612280) p38 MAPK (pT180/pY182), Phospho-Specific BD Pharmingen M (IgG1; 30)
p38 MAPK (pT180/pY182), Phospho-Specific (612288) p38 MAPK (pT180/pY182), Phospho-Specific BD Pharmingen M (IgG1; 36)
Y182 Y184 EMTGpYVATR 11 11, 34
p38 MAPK (pT180/pY182), Phospho-Specific (612280) p38 MAPK (pT180/pY182), Phospho-Specific BD Pharmingen M (IgG1; 30)
p38 MAPK (pT180/pY182), Phospho-Specific (612288) p38 MAPK (pT180/pY182), Phospho-Specific BD Pharmingen M (IgG1; 36)
TP53
p53
Genbank LocusLink 		Alt. gene names: p53; cellular tumor antigen p53; phosphoprotein p53;
S37 SPLPpSQAMD 104
p53 (pS37) (558369) p53 (pS37) BD Pharmingen M (IgG1; J159-641.15.4)
S46 DLMLpSPDDI 131 5
p53 (pS46) (558245) p53 (pS46) BD Pharmingen M (IgG1; I117-1091)
RPS6KA1
p90-RSK1
Genbank LocusLink 		Alt. gene names: ribosomal protein S6 kinase, 90kD, polypeptide 1; p90-RSK1; MAPKAP-K1;
S380 FRGFpSFVAT 318
p90 RSK1 (pS380) (612692) p90 RSK1 (pS380) BD Pharmingen M (IgG1; 20a)
PXN
paxillin
Genbank LocusLink 		Alt. gene names: paxillin; PXN;
Y118 EEHVpYSFPN 43
Paxillin (pY118), Phospho-Specific (611724) Paxillin (pY118), Phospho-Specific BD Pharmingen M (IgG1; 30)
PDGFRB
PDGFR-beta
Genbank LocusLink 		Alt. gene names: CD140b; beta platelet-derived growth factor receptor; PDGFb;
Y771 ESSNpYMAPY 374
CD140b (pY771) (PDGF Receptor b) (558361) CD140b (pY771) (PDGF Receptor b) BD Pharmingen M (IgG1; J23-618)
Y857 RDSNpYISKG 375
CD140b (pY857) (PDGF Receptor b) (558360) CD140b (pY857) (PDGF Receptor b) BD Pharmingen M (IgG1; J24-425)
Y1009 SSVLpYTAVQ 372
CD140b (pY1009) (PDGF Receptor b) (558321) CD140b (pY1009) (PDGF Receptor b) BD Pharmingen M (IgG2b; J25-602)
Y1021 GDNDpYIIPL 373
CD140b (pY1021) (PDGF Receptor b) (558358) CD140b (pY1021) (PDGF Receptor b) BD Pharmingen M (IgG1; J105-412)
PDPK1
PDK1
Genbank LocusLink 		Alt. gene names: upstream PKB kinase; USK; Protein kinase B kinase; PKBK; phospholipid-dependent protein kinase 1; PDK1; 3-phosphoinositide dependent protein kinase-1; phosphorylated by 3-phosphoinositide-dependent kinase 1;
S241 ARANpSFVGT 88
PDPK1 (pS241) (PDK1) (558395) PDPK1 (pS241) (PDK1) BD Pharmingen M (IgG1; J66-653.44.22)
PRKAR2A
PKA RII alpha
Genbank LocusLink 		Alt. gene names: PKA regulatory subunit Type II alpha; PKA RII alpha;
S99 S96 NRRVpSVCAE 381
PKARIIa (pS99) (558244) PKARIIa (pS99) BD Pharmingen M (IgG1; I65-856.286)
PRKAR2B
PKA RII beta
Genbank LocusLink 		Alt. gene names: PKA regulatory subunit Type II beta; PKA RII beta;
S114 TRRApSVCAE 319
PKARIIb (pS114), Phospho-Specific (612550) PKARIIb (pS114), Phospho-Specific BD Pharmingen M (IgG1; 47)
PRKCA
pkc-alpha
Genbank LocusLink 		Alt. gene names: pkc-alpha;
T497 VTTRpTFCGT 360
PKCa (pT497) (558379) PKCa (pT497) BD Pharmingen M (IgG1; K14-984)
T638 QPVLpTPPDQ 190 3
PKCa (pT638) (612699) PKCa (pT638) BD Pharmingen M (IgG1; 35)
PRKCB1
PKC-beta1
Genbank LocusLink 		Alt. gene names: pkc-beta; PRKCB1;
T642 PVELpTPTDK 296 8, 36
PKCa (pT638) (612699) PKCa (pT638) BD Pharmingen M (IgG1; 35)
PLCG1
PLC-gamma 1
Genbank LocusLink 		Alt. gene names: phospholipase C gamma-1; PLC-gamma 1; PLC1; PLCG1; phospholipase C-148; PLC148;
Y783 NPGFpYVEAN 238
Phospholipase Cg (pY783), Phospho-Specific (612464) Phospholipase Cg (pY783), Phospho-Specific BD Pharmingen M (IgG1; 27)
PLC-g1 (Y783) (557883) PLC-g1 (Y783) BD Pharmingen M (IgG1; 20a/PLCGMA1)
PLK1
PLK-1
Genbank LocusLink 		Alt. gene names: Polo-Like Kinase-1; PLK-1; PLK; serine-threonine protein kinase 13; stpk13;
T210 ERKKpTLCGT 382
PLK1 (pT210) (558400) PLK1 (pT210) BD Pharmingen M (IgG1; K50-483)
PKN1
PRK1
Genbank LocusLink 		Alt. gene names: Protein kinase C-related kinase-1; PRK1; protein kinase N; PKN; PKNalpha;
T774 DRTSpTFCGT 195 6, 9, 10
PRK1 (pT774) / PRK2 (pT816) (558399) PRK1 (pT774) / PRK2 (pT816) BD Pharmingen M (IgG1; I85-1151)
PKN2
PRK2
Genbank LocusLink 		Alt. gene names: Protein kinase C-related kinase-2; PRK2;
T816 DRTSpTFCGT 196
PRK1 (pT774) / PRK2 (pT816) (558399) PRK1 (pT774) / PRK2 (pT816) BD Pharmingen M (IgG1; I85-1151)
PGR
progesterone receptor
Genbank LocusLink 		Alt. gene names: progesterone receptor;
S190 PRGLpSPARQ 75
Progesterone Receptor (pS190) (558387) Progesterone Receptor (pS190) BD Pharmingen M (IgG1; 1154/F12)
RDX
Radixin
Genbank LocusLink 		Alt. gene names: Radixin;
T564 T567 DKYKpTLRQI 185
Ezrin (pT567) (558357) Ezrin (pT567) BD Pharmingen M (IgG1; J37-954.281.307)
RB1
rb
Genbank LocusLink 		Alt. gene names: retinoblastoma protein; Rb; pRB;
S780 PPTLpSPIPH 143
Rb (pS780) (558385) Rb (pS780) BD Pharmingen M (IgG1; J146-35)
S807 NIYIpSPLKS 113
Rb (pS807/pS811) (558389) Rb (pS807/pS811) BD Pharmingen M (IgG1; J112-906)
S811 SPLKpSPYKI 114
Rb (pS807/pS811) (558389) Rb (pS807/pS811) BD Pharmingen M (IgG1; J112-906)
RELA
RelA
Genbank LocusLink 		Alt. gene names: reticuloendotheliosis viral oncogene homolog A; RelA; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65); p65Rel; p65;
S522 S536 EDFSpSIADM 134 25
NF-kB p65 (pS536) (558377) NF-kB p65 (pS536) BD Pharmingen M (IgG1; J144-460)
S529 NGLLpSGDED 346
NF-kB (pS529) (558393) NF-kB (pS529) BD Pharmingen M (IgG2b; K10-895.12.50)
LCP2
slp-76
Genbank LocusLink 		Alt. gene names: SH2 domain-containing leukocyte protein of 76kD; slp-76; lymphocyte cytosolic protein 2; LCP2; 76-kDa phosphoprotein; pp76; 76-kDa Vav-associated protein;
Y113 EEDDpYESPN 384
SLP-76 (pY113) (558388) SLP-76 (pY113) BD Pharmingen M (IgG1; J80-373)
Y128 DDGDpYESPN 385
SLP-76 (pY128) (558367) SLP-76 (pY128) BD Pharmingen M (IgG1; J141-668.36.58)
Y145 DDADpYEPPP 387
SLP-76 (pY145) (558362) SLP-76 (pY145) BD Pharmingen M (IgG1; J81-1214.48)
STAT1
STAT1
Genbank LocusLink 		Alt. gene names: signal transducer and activator of transcription 1; STAT1; transcription factor ISGF-3 components p91/p84; ISGF-3;
Y701 KGTGpYIKTE 49
Stat1 (pY701), Phospho-Specific (612132) Stat1 (pY701), Phospho-Specific BD Pharmingen M (IgG1; 14)
Stat1 (pY701), Phospho-Specific (612233) Stat1 (pY701), Phospho-Specific BD Pharmingen M (IgG2a; 4a)
STAT2
STAT2
Genbank LocusLink 		Alt. gene names: Signal Transducers and Activators of Transcription 2; STAT2;
Y690 ERRKpYLKHR 386
Stat2 (pY690) (558095) Stat2 (pY690) BD Pharmingen M (IgG1; 7a/Stat2 (pY690))
STAT3
STAT3
Genbank LocusLink 		Alt. gene names: signal transducer and activator of transcription; STAT3; acute-phase response factor; APRF;
Y705 SAAPpYLKTK 52
SAAPpYLKTK (612357) SAAPpYLKTK BD Pharmingen M (IgG2a; 4)
S727 DLPMpSPRTL 51
Stat3 (pS727), Phospho-Specific (612542) Stat3 (pS727), Phospho-Specific BD Pharmingen M (IgG1; 49)
STAT4
STAT4
Genbank LocusLink 		Alt. gene names: Signal Transducers and Activators of Transcription 4; STAT4;
Y693 GDKGpYVPSV 320
Stat4 (pY693) (612738) Stat4 (pY693) BD Pharmingen M (IgG2b; 38)
STAT5A
STAT5A
Genbank LocusLink 		Alt. gene names: Signal Transducers and Activators of Transcription 5a; STAT5A; mammary gland factor; MGF;
Y694 AVDGpYVKPQ 311
Stat5 (pY694), Phospho-Specific (611818) Stat5 (pY694), Phospho-Specific BD Pharmingen P (Rabbit)
Stat5 (pY694), Phospho-Specific (611964) Stat5 (pY694), Phospho-Specific BD Pharmingen M (IgG1; 47)
STAT5B
STAT5B
Genbank LocusLink 		Alt. gene names: Signal Transducers and Activators of Transcription 5b; STAT5B;
Y699 AVDGpYVKPQ 583
Stat5 (pY694), Phospho-Specific (611818) Stat5 (pY694), Phospho-Specific BD Pharmingen P (Rabbit)
Stat5 (pY694), Phospho-Specific (611964) Stat5 (pY694), Phospho-Specific BD Pharmingen M (IgG1; 47)
STAT6
STAT6
Genbank LocusLink 		Alt. gene names: Signal Transducers and Activators of Transcription 6; STAT6;
Y641 DGRGpYVPAT 249
Stat6 (pY641) (558241) Stat6 (pY641) BD Pharmingen M (IgG1; J71-773.58.11)
Stat6 (pY641), Phospho-Specific (611566) Stat6 (pY641), Phospho-Specific BD Pharmingen M (IgG2a; 18)
Stat6 (pY641), Phospho-Specific (611820) Stat6 (pY641), Phospho-Specific BD Pharmingen P (Rabbit)
SYK
syk
Genbank LocusLink 		Alt. gene names: spleen tyrosine kinase; syk; p72syk;
Y348 DTEVpYESPY 388
Syk (pY348) (558167) Syk (pY348) BD Pharmingen M (IgG1; I120-722)
Y352 YESPpYADPE 321
ZAP-70 (pY319)/Syk (pY352), Phospho-Specific (612574) ZAP-70 (pY319)/Syk (pY352), Phospho-Specific BD Pharmingen M (IgG1; 17a)
CD3Z
TCR-zeta
Genbank LocusLink 		Alt. gene names: TCR-zeta;
Y141 Y142 HDGLpYQGLS 376
CD247 (pY142) (CD3z) (558402) CD247 (pY142) (CD3z) BD Pharmingen M (IgG2a; K25-407.69)
TYK2
tyk2
Genbank LocusLink 		Alt. gene names: non-receptor tyrosine kinase 2; tyk2;
Y1054 EGHEpYYRVR 199
Tyk2 (pY1054/pY1055) (558394) Tyk2 (pY1054/pY1055) BD Pharmingen M (IgG1; I114-617)
Y1055 GHEYpYRVRE 200
Tyk2 (pY1054/pY1055) (558394) Tyk2 (pY1054/pY1055) BD Pharmingen M (IgG1; I114-617)
ZAP70
ZAP-70
Genbank LocusLink 		Alt. gene names: zeta-associated protein 70; zap-70; syk related tyrosine kinase; srk;
Y292 NSDGpYTPEP 390
ZAP-70 (pY292) (558365) ZAP-70 (pY292) BD Pharmingen M (IgG1; J34-602)
Y319 YESPpYSDPE 255
ZAP-70 (pY319)/Syk (pY352), Phospho-Specific (612574) ZAP-70 (pY319)/Syk (pY352), Phospho-Specific BD Pharmingen M (IgG1; 17a)
Y493 DDSYpYTARS 258
ZAP-70 (pY493) (558247) ZAP-70 (pY493) BD Pharmingen M (IgG1; 1a/ZAP70 (pY493))

    References

  1. Balendran A, Casamayor A, Deak M, Paterson A, Gaffney P, Currie R, Downes CP, Alessi DR. 1999. PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2. Curr Biol 9:393-404 (Abstract) [site(s): T308]
  2. Blois JT, Mataraza JM, Mecklenbrauker I, Tarakhovsky A, Chiles TC. 2004. B cell receptor-induced cAMP-response element binding protein activation in B lymphocytes requires novel protein kinase C-delta. J Biol Chem : (Abstract) [site(s): S133]
  3. Bornancin F, Parker PJ. 1996. Phosphorylation of threonine 638 critically controls the dephosphorylation and inactivation of protein kinase Calpha. Curr Biol 6:1114-1123 (Abstract) [site(s): T638]
  4. Brognard J, Clark AS, Ni Y, Dennis PA. 2001. Akt/protein kinase B is constitutively active in non-small cell lung cancer cells and promotes cellular survival and resistance to chemotherapy and radiation. Cancer Res 61:3986-3997 (Abstract) [site(s): T308,S473]
  5. D'Orazi G, Cecchinelli B, Bruno T, Manni I, Higashimoto Y, Saito S, Gostissa M, Coen S, Marchetti A, Del Sal G, Piaggio G, Fanciulli M, Appella E, Soddu S. 2002. Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis. Nat Cell Biol 4:11-19 (Abstract) [site(s): S46]
  6. Dong LQ, Landa LR, Wick MJ, Zhu L, Mukai H, Ono Y, Liu F. 2000. Phosphorylation of protein kinase N by phosphoinositide-dependent protein kinase-1 mediates insulin signals to the actin cytoskeleton. Proc Natl Acad Sci U S A 97:5089-5094 (Abstract) [site(s): T774]
  7. Fleming I, Busse R. 2003. Molecular mechanisms involved in the regulation of the endothelial nitric oxide synthase. Am J Physiol Regul Integr Comp Physiol 284:R1-R12 (Abstract) [site(s): S1177]
  8. Flint AJ, Paladini RD, Koshland DE Jr. 1990. Autophosphorylation of protein kinase C at three separated regions of its primary sequence. Science 249:408-411 (Abstract) [site(s): T642]
  9. Flynn P, Mellor H, Casamassima A, Parker PJ. 2000. Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase. J Biol Chem 275:11064-11070 (Abstract) [site(s): T774]
  10. Gampel A, Parker PJ, Mellor H. 1999. Regulation of epidermal growth factor receptor traffic by the small GTPase rhoB. Curr Biol 9:955-958 (Abstract) [site(s): T774]
  11. Garrington TP, Johnson GL. 1999. Organization and regulation of mitogen-activated protein kinase signaling pathways. Curr Opin Cell Biol 11:211-218 (Abstract) [site(s): T180,Y182]
  12. Gould KL, Nurse P. 1989. Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. Nature 342:39-45 (Abstract) [site(s): Y15]
  13. Gubina E, Luo X, Kwon E, Sakamoto K, Shi YF, Mufson RA. 2001. betac Cytokine Receptor-Induced Stimulation of cAMP Response Element Binding Protein Phosphorylation Requires Protein Kinase C In Myeloid Cells: A Novel Cytokine Signal Transduction Cascade. J Immunol 167:4303-4310 (Abstract) [site(s): S133]
  14. Kitaura J, Asai K, Maeda-Yamamoto M, Kawakami Y, Kikkawa U, Kawakami T. 2000. Akt-dependent cytokine production in mast cells. J Exp Med 192:729-740 (Abstract) [site(s): S473]
  15. Kong SK, Yim MB, Stadtman ER, Chock PB. 1996. Peroxynitrite disables the tyrosine phosphorylation regulatory mechanism: Lymphocyte-specific tyrosine kinase fails to phosphorylate nitrated cdc2(6-20)NH2 peptide. Proc Natl Acad Sci U S A 93:3377-3382 (Abstract) [site(s): Y15]
  16. Krek W, Nigg EA. 1991. Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites. EMBO J 10:305-316 (Abstract) [site(s): Y15]
  17. Kroner C, Eybrechts K, Akkerman JW. 2000. Dual regulation of platelet protein kinase B. J Biol Chem 275:27790-27798 (Abstract) [site(s): T308,S473]
  18. Lynch DK, Ellis CA, Edwards PA, Hiles ID. 1999. Integrin-linked kinase regulates phosphorylation of serine 473 of protein kinase B by an indirect mechanism. Oncogene 18:8024-8032 (Abstract) [site(s): S473]
  19. Murai T, Nakagawa Y, Maeda H, Terada K. 2001. Altered regulation of cell cycle machinery involved in interleukin-1-induced G(1) and G(2) phase growth arrest of A375S2 human melanoma cells. J Biol Chem 276:6797-6806 (Abstract) [site(s): Y15]
  20. Muthusamy N, Leiden JM. 1998. A protein kinase C-, Ras-, and RSK2-dependent signal transduction pathway activates the cAMP-responsive element-binding protein transcription factor following T cell receptor engagement. J Biol Chem 273:22841-22847 (Abstract) [site(s): S133]
  21. Muthusamy N, Leiden JM. 1998. A protein kinase C-, Ras-, and RSK2-dependent signal transduction pathway activates the cAMP-responsive element-binding protein transcription factor following T cell receptor engagement. J Biol Chem 273:22841-22847 (PMID: 9712919) (Abstract) [site(s): S133]
  22. Perez-Villar JJ, Whitney GS, Sitnick MT, Venkatesan S, O'Day K, Schieven GL, Lin TA, Kanner SB. 2002. Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav. Biochemistry 41:10732-10740 (Abstract) [site(s): Y200]
  23. Prowse CN, Deal MS, Lew J. 2001. The complete pathway for catalytic activation of the mitogen-activated protein kinase, ERK2. J Biol Chem 276:40817-40823 (Abstract) [site(s): T185]
  24. Rane MJ, Coxon PY, Powell DW, Webster R, Klein JB, Ping P, Pierce W, McLeish KR. 2000. p38 KINASE-DEPENDENT MAPKAPK-2 ACTIVATION FUNCTIONS AS PDK2 FOR AKT IN HUMAN NEUTROPHILS. J Biol Chem : (Abstract) [site(s): S473]
  25. Sakurai H, Chiba H, Miyoshi H, Sugita T, Toriumi W. 1999. IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain. J Biol Chem 274:30353-30356 (Abstract) [site(s): S522]
  26. Sebastian B, Kakizuka A, Hunter T. 1993. Cdc25M2 activation of cyclin-dependent kinases by dephosphorylation of threonine-14 and tyrosine-15. Proc Natl Acad Sci U S A 90:3521-3524 (Abstract) [site(s): Y15]
  27. Shan X, Czar MJ, Bunnell SC, Liu P, Liu Y, Schwartzberg PL, Wange RL. 2000. Deficiency of PTEN in Jurkat T cells causes constitutive localization of Itk to the plasma membrane and hyperresponsiveness to CD3 stimulation. Mol Cell Biol 20:6945-6957 (Abstract) [site(s): S473]
  28. Shaywitz AJ, Greenberg ME. 1999. CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem 68:821-861 (Abstract) [site(s): S133]
  29. Shikata Y, Birukov KG, Birukova AA, Verin A, Garcia JG. 2003. Involvement of site-specific FAK phosphorylation in sphingosine-1 phosphate- and thrombin-induced focal adhesion remodeling: role of Src and GIT. FASEB J 17:2240-9 (Abstract) [site(s): Y397]
  30. Solomou EE, Juang YT, Tsokos GC. 2001. Protein kinase c-theta participates in the activation of cyclic amp-responsive element-binding protein and its subsequent binding to the -180 site of the il-2 promoter in normal human t lymphocytes. J Immunol 166:5665-5674 (Abstract) [site(s): S133]
  31. Stokoe D, Stephens LR, Copeland T, Gaffney PR, Reese CB, Painter GF, Holmes AB, McCormick F, Hawkins PT. 1997. Dual role of phosphatidylinositol-3,4,5-trisphosphate in the activation of protein kinase B. Science 277:567-570 (Abstract) [site(s): S473]
  32. Toker A, Newton AC. 2000. Akt/protein kinase B is regulated by autophosphorylation at the hypothetical PDK-2 site. J Biol Chem 275:8271-8274 (Abstract) [site(s): S473]
  33. Wang D, Huang XY, Cole PA. 2001. Molecular determinants for Csk-catalyzed tyrosine phosphorylation of the Src tail. Biochemistry 40:2004-2010 (Abstract) [site(s): Y530]
  34. Widmann C, Gibson S, Jarpe MB, Johnson GL. 1999. Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human. Physiol Rev 79:143-180 (Abstract) [site(s): T180,Y182]
  35. Xie H, Rothstein TL. 1995. Protein kinase C mediates activation of nuclear cAMP response element-binding protein (CREB) in B lymphocytes stimulated through surface Ig. J Immunol 154:1717-23 (Abstract) [site(s): S133]
  36. Zhang J, Wang L, Schwartz J, Bond RW, Bishop WR. 1994. Phosphorylation of Thr642 is an early event in the processing of newly synthesized protein kinase C beta 1 and is essential for its activation. J Biol Chem 269:19578-19584 (Abstract) [site(s): T642]