Rockland

Antibodies and their Designated Sites (count: 36)

Gene/Protein	Phosphorylation Sites					Antibodies
Symbol Name	Site Name	Alt Res Names	Local Sequence	MPR Site ID	Refs	Antibody Name (Cat#)	Co.	Polyclonal (species) OR Mono. (isotype; clone)
AKT1 Akt1	Alt. gene names: protein Kinase B; PKB; Akt1; AKT; C-AKT; RAC-alpha serine/threonine kinase; RAC; rac alpha; rac-pk alpha;
	T308		ATMKpTFCGT	277	3, 5, 9
	T308		ATMKpTFCGT	277	3, 5, 9	Anti-AKT phospho specific pT308 RABBIT (600-401-269)		P (Rabbit)
	S473		FPQFpSYSAS	12	5, 8, 9, 11, 14, 15, 18, 19
	S473		FPQFpSYSAS	12	5, 8, 9, 11, 14, 15, 18, 19	Anti-AKT phospho specific pS473 RABBIT (600-401-268)		P (Rabbit)
ANAPC1 APC1	Alt. gene names: anaphase-promoting complex 1;
	S355		SRAHpSPALG	353
	S355		SRAHpSPALG	353		Anti-Human APC1 pS355 (600-401-856)		P (Rabbit)
	S377		SHNQpSPKRH	354
	S377		SHNQpSPKRH	354		Anti-Apc1 pS377 (Rabbit) (600-401-877)		P (Rabbit)
CDC16 APC6	Alt. gene names: Anaphase promoting complex 6; cdc16 homolog;
	T580		ETGLpTPLET	355
	T580		ETGLpTPLET	355		Anti-Human Apc6 pT580 (Rabbit) (600-401-876)		P (Rabbit)
DDEF1 ASAP1	Alt. gene names: development and differentiation enhancing factor 1; DDEF1; ASAP1;
	Y767		QRLSpYGAFT	357
	Y767		QRLSpYGAFT	357		Anti-ASAP1 pY782 (Rabbit) (600-401-910)		P (Rabbit)
MAP3K5 ASK-1	Alt. gene names: MEKK5; Apoptosis signal-regulating kinase 1; ASK-1; ASK; MAPKKK5; MAP3K5;
	S83		GRGSpSVGGG	339
	S83		GRGSpSVGGG	339		Anti-Human ASK-1 phospho specific pS83 RABBIT (200-401-410)		P (Rabbit)
ATM ATM	Alt. gene names: ataxia-telangiectasia mutated; ATM; ATM kinase;
	S1981	S1987	FEEGpSQSTT	407
						Anti-ATM Protein Kinase pS1981 [Mouse] (200-301-400)		M (G1K; 10H11.E12)
						Anti-ATM Protein Kinase pS1981 [Rabbit] (600-401-400)		P (Rabbit)
RAF1 c-Raf	Alt. gene names: c-Raf; raf-1; mil; Raf1;
	S259		QRSTpSTPNV	92
	S259		QRSTpSTPNV	92		Anti-Human Raf-1 pS239 (Rabbit) (200-401-451)		P (Rabbit)
CDC27 cdc27	Alt. gene names: cdc27;
	T244		ISPDpTVPLG	356
	T244		ISPDpTVPLG	356		Anti-CDC27 pT244 [Rabbit] (600-401-885)		P (Rabbit)
CHEK2 Chk2	Alt. gene names: Chk2; Rad53;
	T68		ETVSpTQELY	192	1
	T68		ETVSpTQELY	192	1	Anti-Human CHK2 pT68 (Rabbit) (600-401-280)		P (Rabbit)
CREB1 CREB-alpha	Alt. gene names: cyclic AMP response element binding protein 1; CREB; CREB 1;
	S133		SRRPpSYRKI	265	4, 6, 12, 13, 16, 17, 20
	S133		SRRPpSYRKI	265	4, 6, 12, 13, 16, 17, 20	Anti-CREB phospho specific pS133 [Rabbit] (600-401-270)		P (Rabbit)
CREB1 CREB-delta	Alt. gene names: cyclic AMP response element binding protein 1; CREB; CREB 1;
	S133		SRRPpSYRKI	265	4, 6, 12, 13, 16, 17, 20
	S133		SRRPpSYRKI	265	4, 6, 12, 13, 16, 17, 20	Anti-CREB phospho specific pS133 [Rabbit] (600-401-270)		P (Rabbit)
CCNB1 cyclin B1	Alt. gene names: cyclin B1;
	S126		VDTApSPSPM	336
	S126		VDTApSPSPM	336		Anti-Human Cyclin B1 pS126 (Rabbit) (600-401-464)		P (Rabbit)
PRKDC DNA-PKcs	Alt. gene names: DNA-dependent protein kinase catalytic subunit; DNA-PKcs; DNA-PK;
	T2609		MFVEpTQASQ	351
	T2609		MFVEpTQASQ	351		Anti-DNA PKcs pT 2609 (Rabbit) (600-401-494)		P (Rabbit)
E2F1 E2F1	Alt. gene names: E2F transcription factor 1;
	S364		SRMGpSLRAP	352
	S364		SRMGpSLRAP	352		Anti-Human E2F-1 pS364 (Rabbit) (600-401-690)		P (Rabbit)
ECT2 ECT2	Alt. gene names: epithelial cell transforming sequence 2; ECT2; Pebble;
	T791	T790	DMDSpTLSRA	760
	T791	T790	DMDSpTLSRA	760		Anti-ECT2 pT790 (Rabbit) (600-401-935)		P (Rabbit)
GYS1 glycogen synthase 1	Alt. gene names: glycogen synthase 1; GYS1; muscle glycogen synthase;
	S641		PRPApSVPPS	341
	S641		PRPApSVPPS	341		Anti-Human Muscle Glycogen Synthase pS640 [Rabbit] (600-401-415)		P (Rabbit)
GSK3A GSK-3alpha	Alt. gene names: glycogen synthase kinase-3 alpha; GSK-3alpha;
	S21		ARTSpSFAEP	82	2
	S21		ARTSpSFAEP	82	2	Anti-Human GSK3 alpha pS21 (Rabbit) (600-401-429)		P (Rabbit)
GSK3B GSK-3beta	Alt. gene names: glycogen synthase kinase-3 beta; GSK-3beta; Zw3;
	S9		PRTTpSFAES	34
	S9		PRTTpSFAES	34		Anti-Human GSK3 beta pS9 (Rabbit) (600-401-424)		P (Rabbit)
HIST1H4D histone H4	Alt. gene names:
	S2		---MpSGRGK	349
	S2		---MpSGRGK	349		Anti-Human Histone H4 pS1 (Rabbit) (600-401-353)		P (Rabbit)
HD huntingtin	Alt. gene names: huntingtin; Huntington;
	S421		SRSGpSIVEL	337
	S421		SRSGpSIVEL	337		Anti-Human Huntington pS421(Rabbit) (600-401-433)		P (Rabbit)
IKBKE IKKepsilon	Alt. gene names: IKK-related kinase epsilon; IKKepsilon;
	T501		SRLRpTLAEV	344
	T501		SRLRpTLAEV	344		Anti-IKKe pT501 [Rabbit] (600-401-267)		P (Rabbit)
IRS1 IRS-1	Alt. gene names: insulin-receptor signaling 1; insulin receptor substrate 1; IRS-1;
	S307	S302	SRTEpSITAT	125
	S307	S302	SRTEpSITAT	125		Anti-Human IRS1 pS307 (Rabbit) (600-401-445)		P (Rabbit)
	S527		KRTHpSAGTS	340
	S527		KRTHpSAGTS	340		Anti-Human IRS1 pS527 (Rabbit) (600-401-434)		P (Rabbit)
NF2 merlin	Alt. gene names: merlin; schwannomin; neurofibromatosis type 2 tumor suppressor gene; NF2;
	S518		MKRLpSMEIE	342
	S518		MKRLpSMEIE	342		Anti-Human NF2 (Merlin) pS518 [Rabbit] (600-401-414)		P (Rabbit)
MRCL3 MLCB	Alt. gene names: myosin, light polypeptide, regulatory, non-sarcomeric; MLCB;
	S19		QRATpSNVFA	499
	S19		QRATpSNVFA	499		Anti- Myosin Light Chain pS20 [Rabbit] (600-401-416)		P (Rabbit)
MLF1IP MLF1 interacting protein	Alt. gene names: MLF1 interacting protein; MLF1IP; CENPU; CENP-U; CENP-U(50); CENP-50; KLIP1; FLJ23468;
	T78		PLHSpTAIYA	762
	T78		PLHSpTAIYA	762		Anti-MLF1 Interacting Protein pT78 (Rabbit) (600-401-930)		P (Rabbit)
MRLC2 MRLC2	Alt. gene names: myosin regulatory light chain 2; MRLC2;
	S20		QRATpSNVFA	549
	S20		QRATpSNVFA	549		Anti- Myosin Light Chain pS20 [Rabbit] (600-401-416)		P (Rabbit)
FRAP1 mTOR	Alt. gene names: FK506 binding protein 12-rapamycin associated protein; FRAP; mammalian target of rapamycin; mTOR; TOR; RAFT1;
	S2448		TRTDpSYSAG	89
	S2448		TRTDpSYSAG	89		Anti-mTOR pS2448 (Rabbit) (600-401-422)		P (Rabbit)
MYL9 MYRL2	Alt. gene names: MYOSIN REGULATORY LIGHT CHAIN 2, Smooth Muscle; MYRL2; MYOSIN REGULATORY LIGHT CHAIN 2, NONSARCOMERIC; light chain 20; LC20;
	S20		QRATpSNVFA	338
	S20		QRATpSNVFA	338		Anti- Myosin Light Chain pS20 [Rabbit] (600-401-416)		P (Rabbit)
PAK1 PAK1	Alt. gene names: alpha-PAK; alpha pak; pak-alpha; p21 activated kinase 1; PAK1; p65pak;
	T423		SKRSpTMVGT	1064	7, 22
	T423		SKRSpTMVGT	1064	7, 22	Anti-Human PAK 1/2/3 pT423 [Rabbit] (600-401-413)		P (Rabbit)
PAK2 PAK2	Alt. gene names: gamma p21-activated kinase; gamma pak; hPAK65; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK2; auto-phosphorylation dependent kinase; auto-kinase;
	T403	T402	SKRSpTMVGT	172	10, 21
	T403	T402	SKRSpTMVGT	172	10, 21	Anti-Human PAK 1/2/3 pT423 [Rabbit] (600-401-413)		P (Rabbit)
PAK3 PAK3	Alt. gene names: beta p21-activated kinase; beta pak; p21-activated kinase 3; PAK3;
	T421		SKRSpTMVGT	335
	T421		SKRSpTMVGT	335		Anti-Human PAK 1/2/3 pT423 [Rabbit] (600-401-413)		P (Rabbit)
PDCD4 PDCD4	Alt. gene names: programmed cell death 4 (neoplastic transformation inhibitor); nuclear antigen H731;
	S457		KRFVpSEGDG	761
	S457		KRFVpSEGDG	761		Anti-Pdcd4 pS457 (Rabbit) (600-401-964)		P (Rabbit)
RELA RelA	Alt. gene names: reticuloendotheliosis viral oncogene homolog A; RelA; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65); p65Rel; p65;
	S276		LRRPpSDREL	345
	S276		LRRPpSDREL	345		Anti-NFKB p65 (Rel A) phospho specific pS276 RABBIT (100-401-264)		P (Rabbit)
	S529		NGLLpSGDED	346
	S529		NGLLpSGDED	346		Anti-NFKB p65 (Rel A) phospho specific pS529 RABBIT (100-401-266)		P (Rabbit)
SH3BP2 SH3BP2	Alt. gene names: 3BP2; SH3BP2;
	S427		FRSFpSFEKP	343
	S427		FRSFpSFEKP	343		Anti-Human SH3BP2 pS427 (Rabbit) (600-401-449)		P (Rabbit)
SMAD3 SMAD3	Alt. gene names: SMAD3;
	S423		IRCSpSVS--	391
	S423		IRCSpSVS--	391		Anti-Smad3 pS423 pS425 (Rabbit) (600-401-919)		P (Rabbit)
	S425		CSSVpS----	392
	S425		CSSVpS----	392		Anti-Smad3 pS423 pS425 (Rabbit) (600-401-919)		P (Rabbit)
SMC1L1 SMC1	Alt. gene names: SMC1L1; SMC1;
	S957		EEGSpSQGED	870
	S957		EEGSpSQGED	870		Anti-SMC1 pS957 [Mouse] (200-301-397)		M (IgG1?1-39; 5D11G5)

References

Ahn JY, Schwarz JK, Piwnica-Worms H, Canman CE. 2000. Threonine 68 phosphorylation by ataxia telangiectasia mutated is required for efficient activation of Chk2 in response to ionizing radiation. Cancer Res 60:5934-5936 (Abstract) [site(s): T68]
Astoul E, Laurence AD, Totty N, Beer S, Alexander DR, Cantrell DA. 2003. Approaches to define antigen receptor-induced serine kinase signal transduction pathways. J Biol Chem 278:9267-9275 (Abstract) [site(s): S21]
Balendran A, Casamayor A, Deak M, Paterson A, Gaffney P, Currie R, Downes CP, Alessi DR. 1999. PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2. Curr Biol 9:393-404 (Abstract) [site(s): T308]
Blois JT, Mataraza JM, Mecklenbrauker I, Tarakhovsky A, Chiles TC. 2004. B cell receptor-induced cAMP-response element binding protein activation in B lymphocytes requires novel protein kinase C-delta. J Biol Chem : (Abstract) [site(s): S133]
Brognard J, Clark AS, Ni Y, Dennis PA. 2001. Akt/protein kinase B is constitutively active in non-small cell lung cancer cells and promotes cellular survival and resistance to chemotherapy and radiation. Cancer Res 61:3986-3997 (Abstract) [site(s): T308,S473]
Gubina E, Luo X, Kwon E, Sakamoto K, Shi YF, Mufson RA. 2001. betac Cytokine Receptor-Induced Stimulation of cAMP Response Element Binding Protein Phosphorylation Requires Protein Kinase C In Myeloid Cells: A Novel Cytokine Signal Transduction Cascade. J Immunol 167:4303-4310 (Abstract) [site(s): S133]
King CC, Gardiner EM, Zenke FT, Bohl BP, Newton AC, Hemmings BA, Bokoch GM. 2000. p21-activated kinase-1 (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1). J Biol Chem : (Abstract) [site(s): T423]
Kitaura J, Asai K, Maeda-Yamamoto M, Kawakami Y, Kikkawa U, Kawakami T. 2000. Akt-dependent cytokine production in mast cells. J Exp Med 192:729-740 (Abstract) [site(s): S473]
Kroner C, Eybrechts K, Akkerman JW. 2000. Dual regulation of platelet protein kinase B. J Biol Chem 275:27790-27798 (Abstract) [site(s): T308,S473]
Lian JP, Toker A, Badwey JA. 2001. Phosphorylation of the activation loop of gamma p21-activated kinase (gamma-Pak) and related kinases (MSTs) in normal and stressed neutrophils. J Immunol 166:6349-6357 (Abstract) [site(s): T403]
Lynch DK, Ellis CA, Edwards PA, Hiles ID. 1999. Integrin-linked kinase regulates phosphorylation of serine 473 of protein kinase B by an indirect mechanism. Oncogene 18:8024-8032 (Abstract) [site(s): S473]
Muthusamy N, Leiden JM. 1998. A protein kinase C-, Ras-, and RSK2-dependent signal transduction pathway activates the cAMP-responsive element-binding protein transcription factor following T cell receptor engagement. J Biol Chem 273:22841-22847 (Abstract) [site(s): S133]
Muthusamy N, Leiden JM. 1998. A protein kinase C-, Ras-, and RSK2-dependent signal transduction pathway activates the cAMP-responsive element-binding protein transcription factor following T cell receptor engagement. J Biol Chem 273:22841-22847 (PMID: 9712919) (Abstract) [site(s): S133]
Rane MJ, Coxon PY, Powell DW, Webster R, Klein JB, Ping P, Pierce W, McLeish KR. 2000. p38 KINASE-DEPENDENT MAPKAPK-2 ACTIVATION FUNCTIONS AS PDK2 FOR AKT IN HUMAN NEUTROPHILS. J Biol Chem : (Abstract) [site(s): S473]
Shan X, Czar MJ, Bunnell SC, Liu P, Liu Y, Schwartzberg PL, Wange RL. 2000. Deficiency of PTEN in Jurkat T cells causes constitutive localization of Itk to the plasma membrane and hyperresponsiveness to CD3 stimulation. Mol Cell Biol 20:6945-6957 (Abstract) [site(s): S473]
Shaywitz AJ, Greenberg ME. 1999. CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem 68:821-861 (Abstract) [site(s): S133]
Solomou EE, Juang YT, Tsokos GC. 2001. Protein kinase c-theta participates in the activation of cyclic amp-responsive element-binding protein and its subsequent binding to the -180 site of the il-2 promoter in normal human t lymphocytes. J Immunol 166:5665-5674 (Abstract) [site(s): S133]
Stokoe D, Stephens LR, Copeland T, Gaffney PR, Reese CB, Painter GF, Holmes AB, McCormick F, Hawkins PT. 1997. Dual role of phosphatidylinositol-3,4,5-trisphosphate in the activation of protein kinase B. Science 277:567-570 (Abstract) [site(s): S473]
Toker A, Newton AC. 2000. Akt/protein kinase B is regulated by autophosphorylation at the hypothetical PDK-2 site. J Biol Chem 275:8271-8274 (Abstract) [site(s): S473]
Xie H, Rothstein TL. 1995. Protein kinase C mediates activation of nuclear cAMP response element-binding protein (CREB) in B lymphocytes stimulated through surface Ig. J Immunol 154:1717-23 (Abstract) [site(s): S133]
Yu JS, Chen WJ, Ni MH, Chan WH, Yang SD. 1998. Identification of the regulatory autophosphorylation site of autophosphorylation-dependent protein kinase (auto-kinase). Evidence that auto-kinase belongs to a member of the p21-activated kinase family. Biochem J 334:121-131 (Abstract) [site(s): T403]
Zenke FT, King CC, Bohl BP, Bokoch GM. 1999. Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity. J Biol Chem 274:32565-32573 (Abstract) [site(s): T423]

M P R

Mammalian Phosphorylation Resource

Cataloging 1592 antibodies, 1038 phophorylation sites, and 442 proteins.

Presented by the Center for Cancer Research

Rockland

References