Duke University: DetailsSuperfund Basic Research ProgramDevelopmental Effects of Superfund Hydrocarbon Mixtures in Fundulus heteroclitusProject Leaders: Richard T. Di Giulio, David E. Hinton, Marjorie Oleksiak (University of Miami) SummaryPrevious work in this project demonstrated that extracts of sediments from an estuary adjacent to a PAH dominated Superfund site in VA are highly teratogenic to embryos of the killifish, Fundulus heteroclitus, with cardiovascular defects the most pronounced effect observed. Studies with model PAHs demonstrated marked synergistic interactions between PAH-type AHR agonists and CYP1A inhibitors. Current risk assessment approaches for PAHs, however, assume an additive model of toxicity for PAHs. Hypoxia is an important variable affecting development as well as comprising a natural stressor in estuaries. Thus, the overarching goal of this project is to elucidate interactive effects on development and mechanisms underling such effects by PAH mixtures and hypoxia in Fundulus. The objectives are:
This study integrates three complimentary experimental methodologies:
This project elucidates toxic interactions of a class of chemicals (PAHs) that is exhibiting steady increase in the environment, invariably occurs in mixtures, and for which current assessments may underestimate risks for developmental effects. The model for this project, Fundulus, has great ecological relevance, is an excellent sentinel species for coastal ecosystems, and provides a useful model for developmental studies relevant to human health. |
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