Paul N. Hengen, Ph.D. - version 01.11.09.1998 of readme.html- latest update 11 September 1998
Methods and reagents is a unique monthly column consisting of highlights of discussions originally posted to the newsgroup bionet.molbio.methds-reagnts, available on the Internet. Each column focuses on one or two topics of general interest. This joint project among the Elsevier journal Trends in Biochemical Sciences (TIBS), BIOSCI, and NIH represents an exciting new step combining hardcopy and electronic publication. For details on how to partake in the newsgroup, see the introductory article or click here to read postings at the BIOSCI location. To view any of the following manuscripts, click on the BiBTeX identifier and you will see an ASCII version.
Copyright: These manuscripts are not copyrighted by Elsevier Publishing Company. However, you may not reproduce any portion for resale or edit the text for redistribution, sale, or otherwise without written permission from the author.
@article{Hengen1993Novtibs,
author = "P. N. Hengen",
title = "Methods and Reagents - Bummer buffers and lightning ligations",
journal = "Trends in Biochemical Sciences",
volume = "18",
number = "11",
pages = "446-448",
month = "November",
year = "1993"}
@article{Hengen1993Dectibs,
author = "P. N. Hengen",
title = "Methods and Reagents - Hybridization doughnuts and uninvited phages",
journal = "Trends in Biochemical Sciences",
volume = "18",
number = "12",
pages = "484-485",
month = "December",
year = "1993"}
@article{Hengen1994Jantibs,
author = "P. N. Hengen",
title = "Methods and Reagents - Kit Wars",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "1",
pages = "46-47",
month = "January",
year = "1994"}
@article{Hengen1994Febtibs, HTML version
author = "P. N. Hengen",
title = "Methods and Reagents - Determining DNA concentrations
and rescuing {PCR} primers",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "2",
pages = "93-94",
month = "February",
year = "1994"}
@article{Hengen1994Martibs,
author = "P. N. Hengen",
title = "Methods and Reagents - Ghost plasmid of pBluescript",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "3",
pages = "139-140",
month = "March",
year = "1994"}
@article{Hengen1994Aprtibs,
author = "P. N. Hengen",
title = "Methods and Reagents - On the magic of mini-preps...",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "4",
pages = "182-183",
month = "April",
year = "1994"}
@article{Hengen1994Maytibs,
author = "P. N. Hengen",
title = "Methods and Reagents - Use of {${}^{33}{\mbox{P}}$}:
a safer way to sequence?",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "5",
pages = "227-229",
month = "May",
year = "1994"}
@article{Hengen1994Juntibs,
author = "P. N. Hengen",
title = "Methods and Reagents - Disposal of ethidium bromide",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "6",
pages = "257-258",
month = "June",
year = "1994"}
@article{Hengen1994Jultibs,
author = "P. N. Hengen",
title = "Methods and reagents - Slants and spots",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "7",
pages = "300-301",
month = "July",
year = "1994"}
@article{Hengen1994Augtibs, HTML version
author = "P. N. Hengen",
title = "Methods and reagents - Long and accurate {PCR}",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "8",
pages = "341-342",
month = "August",
comment = "LA-PCR FAQ and Info Sheet",
year = "1994"}
@article{Hengen1994Septibs,
author = "P. N. Hengen",
title = "Methods and reagents - Recovering {DNA} from agarose gels",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "9",
pages = "388-389",
month = "September",
year = "1994"}
@article{Hengen1994Octtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Better competent cells and
{DNA} polymerase contaminants",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "10",
pages = "426-427",
month = "October",
year = "1994"}
@article{Hengen1994Dectibs,
author = "P. N. Hengen",
title = "Methods and reagents - Glowing blue gels and nuked nucleases",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "12",
pages = "556-557",
month = "December",
year = "1994"}
@article{Hengen1995Jantibs, HTML version
author = "P. N. Hengen",
title = "Methods and reagents - Wayward {PCR} primers",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "1",
pages = "42-44",
month = "January",
year = "1995"}
@article{Hengen1995Febtibs, HTML version
author = "P. N. Hengen",
title = "Methods and reagents - Cloning {PCR} products using {T}-vectors",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "2",
pages = "85-86",
month = "February",
year = "1995"}
@article{Hengen1995Martibs, HTML version
author = "P. N. Hengen",
title = "Methods and reagents - Reamplification of {PCR} fragments",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "3",
pages = "124-125",
month = "March",
year = "1995"}
@article{Hengen1995Aprtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Caring for your hybridization membranes",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "4",
pages = "160-161",
month = "April",
year = "1995"}
@article{Hengen1995Maytibs,
author = "P. N. Hengen",
title = "Methods and reagents - Agarose gel electrophoresis in your kitchen",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "5",
pages = "202-203",
month = "May",
year = "1995"}
@article{Hengen1995Juntibs,
author = "P. N. Hengen",
title = "Methods and reagents - Electro-transformation of
{{\em Escherichia coli}} with plasmid {DNA}",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "6",
pages = "248-249"
month = "June",
year = "1995"}
@article{Hengen1995Jultibs,
author = "P. N. Hengen",
title = "Methods and reagents - Purification of {His-Tag} fusion
proteins from {{\em Escherichia coli}}",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "7",
pages = "285-286",
month = "July",
year = "1995"}
@article{Hengen1995Augtibs, HTML version
author = "P. N. Hengen",
title = "Methods and reagents - Fidelity of {DNA} polymerases for {PCR}",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "8",
pages = "324-325",
month = "August",
year = "1995"}
@article{Hengen1995Septibs,
author = "P. N. Hengen",
title = "Methods and reagents - Vectorette, splinkerette,
and boomerang {DNA} amplification",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "9",
pages = "372-373",
month = "September",
comment = "Fig.1,Kevin Ahern (ahernk@ucs.orst.edu),
Kevin's original figure, Boomerang US Patent Number 5,470,724",
year = "1995"}
@article{Hengen1995Novtibs, HTML version
author = "P. N. Hengen",
title = "Methods and reagents - Quantitative {PCR} - an
accurate measure of {mRNA}?",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "11",
pages = "476-477",
month = "November",
year = "1995"}
@article{Hengen1995Dectibs,
author = "P. N. Hengen",
title = "Methods and reagents - Floaties in the gene pool",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "12",
pages = "522-523",
month = "December",
year = "1995"}
@article{Hengen1996Jantibs,
author = "P. N. Hengen",
title = "Methods and reagents - Cycle sequencing through {GC}--rich regions",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "1",
pages = "33-34",
month = "January",
year = "1996"}
@article{Hengen1996Febtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Preparing ultra-competent
{{\em Escherichia coli}}",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "2",
pages = "75-76",
month = "February",
year = "1996"}
@article{Hengen1996Martibs,
author = "P. N. Hengen",
title = "Methods and reagents - Is {RNase--free} really
{RNase} for free?",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "3",
pages = "112-113",
month = "March",
year = "1996"}
@article{Hengen1996Aprtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Is there any sense
in antisense terminology?",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "4",
pages = "153-154",
month = "April",
year = "1996"}
@article{Hengen1996Maytibs,
author = "P. N. Hengen",
title = "Methods and reagents - Eliminating banding
artifacts from {SDS--PAGE}",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "5",
pages = "191-193",
month = "May",
year = "1996"}
@article{Hengen1996Juntibs,
author = "P. N. Hengen",
title = "Methods and reagents - Carriers for precipitating
nucleic acids",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "6",
pages = "224-225",
month = "June",
year = "1996"}
@article{Hengen1996Jultibs,
author = "P. N. Hengen",
title = "Methods and reagents - Pouring sequencing gels the
old fashioned way",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "7",
pages = "273-274",
month = "July",
year = "1996"}
@article{Hengen1996Augtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Is your fluorometer on the blink?",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "8",
pages = "317-318",
month = "August",
year = "1996"}
@article{Hengen1996Octtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Purification of {GST} fusion proteins",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "10",
pages = "400-401",
month = "October",
year = "1996"}
@article{Hengen1996Novtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Eliminating ghost bands from plasmid preps",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "11",
pages = "441-442",
month = "November",
year = "1996"}
@article{Hengen1996Dectibs,
author = "P. N. Hengen",
title = "Methods and reagents - Expression profiling using
messenger {RNA} assays",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "12",
pages = "492-493",
month = "December",
year = "1996"}
@article{Hengen1997Jantcb,
author = "P. N. Hengen",
title = "Internet newsgroups",
journal = "Trends in Cell Biology",
volume = "7",
number = "1",
pages = "34-35",
month = "January",
year = "1997"}
@article{Hengen1997Jantibs,
author = "P. N. Hengen",
title = "Methods and reagents - False positives from the yeast
two--hybrid system",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "1",
pages = "33-34",
month = "January",
year = "1997"}
@article{Hengen1997Febtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Emergency sterilization using microwaves",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "2",
pages = "68-69",
month = "February",
year = "1997"}
@article{Hengen1997Martibs,
author = "P. N. Hengen",
title = "Methods and reagents - Reducing background colonies
with positive selection vectors",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "3",
pages = "105-106",
month = "March",
year = "1997"}
@article{Hengen1997Aprtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Degraded {DNA} and gel tornados",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "4",
pages = "138-139",
month = "April",
year = "1997"}
@article{Hengen1997Maytibs,
author = "P. N. Hengen",
title = "Methods and reagents - Protecting vector {DNA} from {UV} light",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "5",
pages = "182-183",
month = "May",
year = "1997"}
@article{Hengen1997Junetibs, HTML version
author = "P. N. Hengen",
title = "Methods and reagents - Optimizing multiplex and {LA-PCR}
with betaine",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "6",
pages = "225-226",
month = "June",
year = "1997"}
@article{Hengen1997Jultibs,
author = "P. N. Hengen",
title = "Methods and reagents - Shearing {DNA} for genomic
library construction",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "7",
pages = "273-274",
month = "July",
year = "1997"}
@article{Hengen1997Augtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Chemiluminescent detection methods",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "8",
pages = "313-314",
month = "August",
year = "1997"}
@article{Hengen1997Septibs,
author = "P. N. Hengen",
title = "Methods and reagents - bionet.molbio.methds-reagents - a status report",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "9",
pages = "359-360",
month = "September",
comment = "The final article...Adios Amoebas!",
year = "1997"}
FAQ (Frequently Asked Questions) for the bionet.molbio.methds-reagnts news group