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Model Characteristics - Model:
ARR2PBi-caFGFR1
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| Model Descriptor |
ARR2PBi-caFGFR1
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| Official Nomenclature |
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| Genotype |
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| Species |
Mouse (Mus musculus)
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| Strain |
FVB/N
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| Is This a Tool Strain? |
No
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Developmental Stage
(applies only to Zebrafish) |
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| Experimental Design |
Young adult FVB mice (6 weeks old) were purchased from Charles River, and young adult Swiss/Webster mice were from Harlan Sprague Dawley. The cDNA encoding for FLAG epitope-tagged caFGFR1 was constructed and inserted in the SSI vector. A transgene-specific sense primer pFLAG1 and a common FGFR1 antisense primer p240 were used to verify correct insertion and expression of the transgene by PCR. The transgene was excised and purified for pronuclear microinjection. Genomic DNAs were purified from the tails of founder mice at day 7 after birth and screened by PCR. Total RNA was isolated from the mice, and analyzed by reverse transcription-PCR with pFLAG1 and p240 primers.
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| Phenotype |
Disruption of the regulatory communication from the stroma to the epithelium mediated by the FGF7/10-FGFR2 signaling axis in the prostate and expression of ectopic FGFR1 in prostatic epithelial cells often correlate with prostate cancer progression both in human and in experimental animals. Ectopic expression of constitutively active FGFR1 mutant (caFGFR1) at low levels in prostate epithelial cells induces low- to intermediate-grade prostatic intraepithelial neoplasia (PIN) within 6–8 months and high-grade PIN in 20–25 months. Depression of the FGFR2 signaling in the prostate also disturbs homeostasis in the prostate and induces prostate hyperplasia. To study whether PIN lesions induced by the caFGFR1 were expression-level dependent, and whether expression of the caFGFR1 and depression of the FGFR2 signaling in the prostate synergistically disturbed prostate homeostasis, we generated two new strains of ARR2PBi-caFGFR1 transgenic mice, which highly expressed caFGFR1 in prostatic epithelial cells. The mice were crossed with KDNR mice to generate ARR2PBi-caFGFR1/KDNR bigenic mice. The ARR2PBi-caFGFR1 mice developed high-grade PIN within 8 months, which was significantly faster than the mice expressing caFGFR1 at low levels. In addition, depression of the FGFR2 signaling clearly promoted perturbation of cellular homeostasis induced by the caFGFR1. The results demonstrated that the PIN development in caFGFR1 transgenic mice was caFGFR1 dosage-dependent, and indicated that the ectopic FGFR1 and the resident FGFR2 in epithelial cells had opposite impacts on intercompartmental homeostasis in the prostate. The bigenic mice provide a model with cooperative aberrations in the fibroblast growth factor signaling axis for evaluation of tumor-initiating events in prostate tumorigenesis.
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| Website for add. info |
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| Breeding Notes |
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| Sex Distribution of the Phenotype |
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| Submitted by |
Wang, Fen
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| Principal Investigator / Lab |
Wang, Fen
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| Comment |
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| Model Availability: This model is available from |
| Strain |
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