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NCI Protocols/Reference Material
Page Updated: Thursday, 03-Mar-2005 10:17:38 EST
Microarray Sample Labeling and Hybridization
RNA Labeling / Microarry Hybridization Protocol: Target Prep/Hyb Protocol (March 2005)
Word
or
PDF
ATC Microarray Workshop Training Manual:
Word
or
PDF
Updated March 2005
Frequently Asked Questions
Word
or
PDF
RNA Amplification Protocols Links
Many amplification kits/protocols have come to market. While the Array Technology Center facility cannot make any recommendations, we have successfully used the Ambion kit.
Ambion
Arcturus
Genisphere
Nugen
Making Sense of Your Microarray Target:
Powerpoint
Microarray Scanning and 1° Analysis
Axon GenePix 4000A Scanner
User's Guide
Note: Restricted to IP addresses within the NIH domain.
GenePix Pro 5.0 Analysis Software User's Guide and Tutorial
, PDF format, requires the Adobe Acrobat
Reader
Note: Restricted to IP addresses within the NIH domain.
GenePix 5.0 Results Tab Column Descriptions
Archaic Microarray Protocols
Direct Incorporation Sample Labeling and Hybridization
Probe
Preparation using PolyA RNA
Probe
Preparation using Total RNA
RNA Amplification Protocols for cDNA Arrays
RNA Amplification Protocol from April 2000
Nature Biotechnology
:
Word
Updated Jan 2005
contact
Ena Wang
with questions and comments.
"Modified Eberwine" Amplification of RNA:
Word
or
PDF
RNA Labeling and Hybridization:
Word
or
PDF
Laboratory Methods
Preparation
of Poly-L-Lysine coated slides
Post Processing
of Printed Arrays
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