NCI Protocols/Reference Material

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NCI Protocols/Reference Material
Page Updated: Thursday, 03-Mar-2005 10:17:38 EST

Microarray Sample Labeling and Hybridization

RNA Labeling / Microarry Hybridization Protocol: Target Prep/Hyb Protocol (March 2005) Word or PDF

ATC Microarray Workshop Training Manual: Word or PDF
Updated March 2005

Frequently Asked Questions Word or PDF

RNA Amplification Protocols Links
Many amplification kits/protocols have come to market. While the Array Technology Center facility cannot make any recommendations, we have successfully used the Ambion kit.

Making Sense of Your Microarray Target: Powerpoint

Microarray Scanning and 1° Analysis

Axon GenePix 4000A Scanner User's Guide
Note: Restricted to IP addresses within the NIH domain.

GenePix Pro 5.0 Analysis Software User's Guide and Tutorial, PDF format, requires the Adobe Acrobat Reader
Note: Restricted to IP addresses within the NIH domain.

GenePix 5.0 Results Tab Column Descriptions

Archaic Microarray Protocols
Direct Incorporation Sample Labeling and Hybridization

Probe Preparation using PolyA RNA
Probe Preparation using Total RNA

RNA Amplification Protocols for cDNA Arrays

RNA Amplification Protocol from April 2000 Nature Biotechnology: Word Updated Jan 2005
contact Ena Wang with questions and comments.

"Modified Eberwine" Amplification of RNA: Word or PDF

RNA Labeling and Hybridization: Word or PDF

Laboratory Methods

Preparation of Poly-L-Lysine coated slides

Post Processing of Printed Arrays

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