You are viewing an old version (v. 74) of this page.
The latest version is v. 77, last edited on Dec 22, 2008
(view differences
|
)
<< View previous version | view page history | view next version >>
<< View previous version | view page history | view next version >>
Antibody Validation Table
Target Protein |
Company |
Recommended Dilution |
Source | Weight | Cross Reactivity |
Positive Control |
Cross Reactivity Validated by Western Blotting (Canine Samples) |
|
---|---|---|---|---|---|---|---|---|
Akt |
COP:Cell Signaling |
1:1000 | Rabbit |
60kDa |
H,M,R |
K7M2 |
COP:Protocol COP:Western Blot-Normal tissue COP: Protocol- Tumor Western Blot-Tumor tissue |
|
pAKT | COP:Cell Signaling |
1:1000 | Rabbit |
60kDa | H,M,R,C,Hm |
K7M2 NIH 3T3 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor Western Blot-Tumor tissue |
|
elF4E |
COP:Cell Signaling |
1:1000 | Rabbit |
25kDa |
H,M,R,Z |
K7M2 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue |
|
P-elF4E |
COP:Cell Signaling |
1:1000 | Rabbit |
25 kDa |
H,M,R,Z |
K7M2 | COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue |
|
Ezrin | COP:Sigma |
1:1000 | Mouse |
80 kDa |
H |
K7M2 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor Western Blot-Tumor tissue |
|
p-Ezrin |
COP:Cell Signaling |
1:1000 | Rabbit |
80 kDa |
K7M2 NIH 3T3 |
COP:Protocol COP:Western Blot-Normal tissue COP: Protocol-Tumor Western Blot-Tumor tissue |
||
ERM |
COP:Cell Signaling |
1:1000 | Rabbit |
75,80 kDa |
H,M,R,MK |
K7M2 A431 |
COP:Protocol COP:Western Blot-Tumor tissue COP:Protocol-Tumor Western Blot-Tumor tissue |
|
P-ERM |
COP:Cell Signaling |
1:1000 | Rabbit |
75, 80 kDa |
H,M,R,Mk |
K7M2 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor Western Blot-Tumor tissue |
|
4E-BP1 | COP:Cell Signaling |
1:1000 | Rabbit |
15-20 kDa |
H,M,R,Z |
K7M2 |
Protocol Western Blot-Normal Tissue Protocol-Tumor Western Blot-Tumor tissue |
|
P-4E-BP1 |
COP:Cell Signaling |
1:1000 | Rabbit |
15-20 kDa |
H,M,R,Z |
K7M2 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue |
|
p44/42 Map Kinase |
COP:Cell Signaling |
1:1000 | Rabbit |
42,44 kDa |
H,M,R,Hm,Z |
K7M2 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue |
|
P-P44/42 Map Kinase |
Cell Signaling |
1:2000 | Mouse |
42,44 kDa |
H,M,R,Hm,Z | K7M2 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue |
|
p70 S6 Kinase |
COP:Cell Signaling |
1:1000 | Mouse |
70,85 kDa |
H,M,R,Mk |
K7M2 NIH 3T3 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue |
|
P-p70 S6 Kinase |
COP:Cell Signaling |
1:1000 | Mouse |
70,85 kDa |
H,M,R,Mk | K7M2 NIH 3T3 |
COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue |
|
mTOR | COP:Cell Signaling |
1:1000 | Rabbit | 210 kDa |
K7M2 | |||
p-mTOR |
COP:Cell Signaling | 1:1000 |
Rabbit | 210 kDa |
K7M2 | |||
P-EGFR | COP:Santa Cruz Biotechnology |
1:200 | Goat | 170 kDa |
A431 + EGF (EPITHELIAL GROWTH FACTOR) |
|||
P-PDGFR | COP:Santa Cruz Biotechnology |
1:200 | Goat | 190 kDa |
A431 | |||
P-BAD | COP:Santa Cruz Biotechnology |
1:200 | Goat | 23 kDa |
A431 |
For any questions please contact Christina Mazcko at mazckoc@mail.nih.gov