Antibody Validation Table

Version 75 by Christina Mazcko
on Dec 22, 2008 12:27.

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Version 76 by Christina Mazcko
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		h3. Antibody Validation Table

		\|\| Target Protein \\ \|\| Company \\ \|\| Recommended Dilution \\ \|\| Source \|\| Weight \|\| Cross Reactivity \\ \|\| Positive Control \\ \|\| Cross Reactivity Validated by Western Blotting (Canine Samples) \\ \|\|
		\| Akt \\ \| [COP:Cell Signaling\|^4058.pdf] \\ \| 1:1000 \| Rabbit \\ \| 60kDa \\ \| H,M,R \\ \| K7M2 \\ \| [COP:Protocol\|^AKT-norm.doc] \\
		[COP:Western Blot-Normal tissue\|^AKT Normal 6.08.06.ppt] \\
		[COP: Protocol\- Tumor\|^AKT-tumor.doc] \\
		Western Blot-Tumor tissue \\ \|
		\| pAKT \| [COP:Cell Signaling \|^9271.pdf] \\ \| 1:1000 \| Rabbit \\ \| 60kDa \| H,M,R,C,Hm \\ \| K7M2 \\
		NIH 3T3 \| [COP:Protocol\|^p-AKT-norm.doc] \\
		[COP:Western Blot-Normal tissue\|^pAKT Normal tissue 6.08.06.ppt] \\
		[COP:Protocol-Tumor\|^p-AKT-tumor.doc] \\
		Western Blot-Tumor tissue \\ \|
		\| elF4E \\ \| [COP:Cell Signaling\|^9742.pdf] \\ \| 1:1000 \| Rabbit \\ \| 25kDa \\ \| H,M,R,Z \\ \| K7M2 \\ \| [COP:Protocol\|^eIF4E norm(3).doc]   \\
		[COP:Western Blot-Normal tissue\|^elF4E Norm 6.22.06.ppt] \\
		[COP:Protocol-Tumor\|^eIF4E tumor.doc] \\
		[COP:Western Blot-Tumor tissue\|^elF4E Tumor 6.23.06.ppt] \\ \|
		\| P-elF4E  \\ \| [COP:Cell Signaling\|^9741.pdf] \\ \| 1:1000 \| Rabbit \\ \| 25 kDa \\ \| H,M,R,Z \\ \| K7M2 \| [COP:Protocol\|^p-eIF4E norm(3).doc] \\
		[COP:Western Blot-Normal tissue\|^P.elF4E norm wphosphotase inhib calyculin.ppt] \\
		[COP:Protocol-Tumor\|^p-eIF4E tumor.doc] \\
		[COP:Western Blot-Tumor tissue\|^p.elF4E Tumor6.23.06.ppt] \\ \|
		\| Ezrin \| [COP:Sigma\|^e8897dat.pdf] \\ \| 1:1000 \| Mouse \\ \| 80 kDa \\ \| H \\ \| K7M2 \\ \| [COP:Protocol\|^ezrin norm.doc] \\
		[COP:Western Blot-Normal tissue\|^Ezrin Normal 6.08.06.ppt] \\
		[COP:Protocol-Tumor\|^ezrin tumor.doc] \\
		Western Blot-Tumor tissue \|
		\| p-Ezrin \\ \| [COP:Cell Signaling\|^3144.pdf] \\ \| 1:1000 \| Rabbit \\ \| 80 kDa \\ \| \| K7M2 \\
		NIH 3T3 \| [COP:Protocol\|^p-ezrin norm.doc]  \\
		[COP:Western Blot-Normal tissue\|^P-Ezrin.tyr363.norm 7.07.06.ppt] \\
		[COP: Protocol-Tumor\|^p-ezrin tumor.doc] \\
		Western Blot-Tumor tissue \\ \|
		\| ERM \\ \| [COP:Cell Signaling\|^3142.pdf] \\ \| 1:1000 \| Rabbit \\ \| 75,80 kDa \\ \| H,M,R,MK \\ \| K7M2 \\
		A431 \| [COP:Protocol\|^erm norm.doc] \\
		[COP:Western Blot-Tumor tissue\|^ERM Normal 6.15.06.ppt] \\
		[COP:Protocol-Tumor\|^erm tumor.doc] \\
		Western Blot-Tumor tissue \\ \|
		\| P-ERM \\ \| [COP:Cell Signaling \|^3141.pdf] \\ \| 1:1000 \| Rabbit \\ \| 75, 80 kDa \\ \| H,M,R,Mk \\ \| K7M2 \\ \| [COP:Protocol\|^p-erm norm.doc] \\
		[COP:Western Blot-Normal tissue\|^p.erm norm 7.06.06.ppt] \\
		[COP:Protocol-Tumor\|^p-erm tumor.doc] \\
		Western Blot-Tumor tissue \\ \|
		\| 4E-BP1 \| [COP:Cell Signaling\|^9452.pdf] \\ \| 1:1000 \| Rabbit \\ \| 15-20 kDa \\ \| H,M,R,Z \\ \| K7M2 \\ \| Protocol \\
		Western Blot-Normal Tissue \\
		Protocol-Tumor \\
		Western Blot-Tumor tissue \| \\ \|
		\| P-4E-BP1 \\ \| [COP:Cell Signaling \|^2855.pdf] \\ \| 1:1000 \| Rabbit \\ \| 15-20 kDa \\ \| H,M,R,Z \\ \| K7M2 \\ \| [COP:Protocol\|^p-4ebp1-norm.doc] \\
		[COP:Western Blot-Normal tissue\|^P.4EBPI w.phosphotase inhib. calyculin.ppt] \\
		[COP:Protocol-Tumor\|^p-4EBP1 TUMOR.doc] \\
		[COP:Western Blot-Tumor tissue\|^p.4ebp1 tumor 6.23.06.ppt] \|
		\| p44/42 Map Kinase \\ \| [COP:Cell Signaling \|^9102.pdf] \\ \| 1:1000 \| Rabbit \\ \| 42,44 kDa \\ \| H,M,R,Hm,Z \\ \| K7M2 \\ \| [COP:Protocol\|^p44.42 map kinase western protocol-norm.doc] \\
		[COP:Western Blot-Normal tissue\|^P44.42 MAPK Normal Tissue 6.14.06.ppt] [COP:Protocol-Tumor\|^p44.42 map kinase western protocol-tumor.doc] \\
		[COP:Western Blot-Tumor tissue\|^P44.42 MAPK Tumor Tissue 6.14.06.ppt] \\ \|
		\| P-P44/42 Map Kinase \\ \| Cell Signaling \\ \| 1:2000 \| Mouse \\ \| 42,44 kDa \\ \| H,M,R,Hm,Z \| K7M2 \\ \| [COP:Protocol\|^p-p44.42 map kinase norm.doc] \\
		[COP:Western Blot-Normal tissue\|^P.p44.42 MAP Kinase NORM 7.06.06 with phosphotase inhib calyculin.ppt] \\
		[COP:Protocol-Tumor\|^p-p44.42 map kinase tumor.doc] \\
		[COP:Western Blot-Tumor tissue\|^p.P44.42 MAPK Tumor 6.14.06.ppt] \\ \|
		\| p70 S6 Kinase \\ \| [COP:Cell Signaling\|^9234.pdf] \\ \| 1:1000 \| Mouse \\ \| 70,85 kDa \\ \| H,M,R,Mk \\ \| K7M2 \\
		NIH 3T3 \| [COP:Protocol\|^p70s6-norm.doc] \\
		[COP:Western Blot-Normal tissue\|^P70s6 Norm 6.21.06.ppt] \\
		[COP:Protocol-Tumor\|^p70s6 tumor.doc]\\
		[COP:Protocol-Tumor\|^p70s6 tumor.doc] \\
		[COP:Western Blot-Tumor tissue\|^p70s6 tumor 6.23.06.ppt] \\ \|
		\| P-p70 S6 Kinase \\ \| [COP:Cell Signaling \|^9204.pdf] \\ \| 1:1000 \| Mouse \\ \| 70,85 kDa \\ \| H,M,R,Mk \| K7M2 \\
		NIH 3T3 \| [COP:Protocol\|^p-p70s6-norm.doc]   \\
		[COP:Western Blot-Normal tissue\|^p.p70s6 norm w. calychin a.ppt] \\
		[COP:Protocol-Tumor\|^p-p70s6 tumor.doc]\\
		[COP:Protocol-Tumor\|^p-p70s6 tumor.doc] \\
		[COP:Western Blot-Tumor tissue\|^p.p70s6 tumor 6.23.06.ppt] \|
		\| mTOR \| [COP:Cell Signaling\|^2972.pdf] \\ \| 1:1000 \| Rabbit \| 210 kDa \\ \| \| K7M2 \| \|
		\| p-mTOR \\ \| [COP:Cell Signaling\|^2971.pdf] \| 1:1000 \\ \| Rabbit \| 210 kDa \\ \| \| K7M2 \|
		\| P-EGFR \| [COP:Santa Cruz Biotechnology\|^sc-16804.pdf] \\ \| 1:200 \| Goat \| 170 kDa \\ \| \| A431 + EGF (EPITHELIAL GROWTH FACTOR) \\ \| \|
		\| P-PDGFR \| [COP:Santa Cruz Biotechnology\|^SC-12910.pdf] \\ \| 1:200 \| Goat \| 190 kDa \\ \| \| A431 \| \|
		\| P-BAD \| [COP:Santa Cruz Biotechnology\|^sc-7998.pdf] \\ \| 1:200 \| Goat \| 23 kDa \\ \| \| A431 \| \|
		For any questions please contact Christina Mazcko at mazckoc@mail.nih.gov