Large B-Cell Lymphoma Profile Data
Raw data for Lymphochip microarrays (Nature 403:503-511, 2000). For each microarray, two scan files
were generated, one for each fluorescence emission wavelength corresponding to the fluorophor
used in the reverse transcription labeling reaction (i.e., Cy3-dUTP = 532 nm, Cy5-dUTP = 635 nm).
Image analysis for each pair of arrays was performed on IBM PC compatible computers using
ScanAlyze, and included a semi-manual gridding process whereby the spots are identified and stored
in a grid file (ScanAlyze SAG file). The extracted data files are available as text tab-delimited DAT
files (ScanAlyze output), and you can find descriptions of the columns here, as well as in the
ScanAlyze User Manual (in Adobe Acrobat PDF Format).
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